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Protein

NH(3)-dependent NAD(+) synthetase

Gene

nadE

Organism
Geobacter lovleyi (strain ATCC BAA-1151 / DSM 17278 / SZ)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses ammonia as a nitrogen source.UniRule annotation

Catalytic activityi

ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+.UniRule annotation

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. NH(3)-dependent NAD(+) synthetase (nadE)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route), the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi40MagnesiumUniRule annotation1
Binding sitei116Deamido-NADUniRule annotation1
Binding sitei136ATPUniRule annotation1
Metal bindingi141MagnesiumUniRule annotation1
Binding sitei165ATPUniRule annotation1
Binding sitei187ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi34 – 41ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Metal-binding, NAD, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00253; UER00333.

Names & Taxonomyi

Protein namesi
Recommended name:
NH(3)-dependent NAD(+) synthetaseUniRule annotation (EC:6.3.1.5UniRule annotation)
Gene namesi
Name:nadEUniRule annotation
Ordered Locus Names:Glov_2228
OrganismiGeobacter lovleyi (strain ATCC BAA-1151 / DSM 17278 / SZ)
Taxonomic identifieri398767 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesGeobacteraceaeGeobacter
Proteomesi
  • UP000002420 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000990231 – 273NH(3)-dependent NAD(+) synthetaseAdd BLAST273

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi398767.Glov_2228.

Structurei

3D structure databases

ProteinModelPortaliB3E4K8.
SMRiB3E4K8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4K. Bacteria.
COG0171. LUCA.
HOGENOMiHOG000238069.
KOiK01916.
OMAiCAINPIG.
OrthoDBiPOG091H00G3.

Family and domain databases

CDDicd00553. NAD_synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00193. NadE_ammonia_dep. 1 hit.
InterProiView protein in InterPro
IPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR022926. NH(3)-dep_NAD(+)_synth.
IPR014729. Rossmann-like_a/b/a_fold.
PANTHERiPTHR23090. PTHR23090. 1 hit.
PfamiView protein in Pfam
PF02540. NAD_synthase. 1 hit.
TIGRFAMsiTIGR00552. nadE. 1 hit.

Sequencei

Sequence statusi: Complete.

B3E4K8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRLNINTKL VRRMLVGFLQ DEIWKVGAKK AVLGLSGGID SALVCHLAAE
60 70 80 90 100
ALGPENVHAI CMPYRTSNPE SEAHARLVAE ASGVQFSVVG ITPMVDAYFD
110 120 130 140 150
QFPDANNMRR GNKMARERMT VLFDHSALYG GLVLGTSNKT ELLLGYGTLY
160 170 180 190 200
GDMASALNPI GDLYKTQVWQ LSEEVGVPKP VIEKKPSADL WAGQTDEEEL
210 220 230 240 250
GFTYREVDEL LYRMVDQRAD TAELVAAGFK QEFVSSIYSK VQNSHFKRRL
260 270
PVIAKVSGRT IDRDFRYSRD WGK
Length:273
Mass (Da):30,412
Last modified:July 22, 2008 - v1
Checksum:iBD0C81CD620363C9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001089 Genomic DNA. Translation: ACD95944.1.
RefSeqiWP_012470280.1. NC_010814.1.

Genome annotation databases

EnsemblBacteriaiACD95944; ACD95944; Glov_2228.
KEGGiglo:Glov_2228.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiNADE_GEOLS
AccessioniPrimary (citable) accession number: B3E4K8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 22, 2008
Last modified: June 7, 2017
This is version 53 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families