B3E4K8 (NADE_GEOLS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	Glov_2228

Organism

Geobacter lovleyi (strain ATCC BAA-1151 / DSM 17278 / SZ) [Complete proteome] [HAMAP]

Taxonomic identifier

398767 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfuromonadales › Geobacteraceae › Geobacter

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 273	273	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000099023
Regions
	Nucleotide binding	34 – 41	8	ATP By similarity
Sites
	Active site	36	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3E4K8 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: BD0C81CD620363C9	FASTA	273	30,412
	10 20 30 40 50 60 MSRLNINTKL VRRMLVGFLQ DEIWKVGAKK AVLGLSGGID SALVCHLAAE ALGPENVHAI 70 80 90 100 110 120 CMPYRTSNPE SEAHARLVAE ASGVQFSVVG ITPMVDAYFD QFPDANNMRR GNKMARERMT 130 140 150 160 170 180 VLFDHSALYG GLVLGTSNKT ELLLGYGTLY GDMASALNPI GDLYKTQVWQ LSEEVGVPKP 190 200 210 220 230 240 VIEKKPSADL WAGQTDEEEL GFTYREVDEL LYRMVDQRAD TAELVAAGFK QEFVSSIYSK 250 260 270 VQNSHFKRRL PVIAKVSGRT IDRDFRYSRD WGK

References

[1]

"Complete sequence of chromosome of Geobacter lovleyi SZ." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Meincke L., Brettin T., Detter J.C., Han C., Tapia R., Kuske C.R., Schmutz J., Larimer F. , Land M., Hauser L., Kyrpides N., Mikhailova N., Sung Y., Fletcher K.E., Ritalahti K.M., Loeffler F.E., Richardson P. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-1151 / DSM 17278 / SZ.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001089 Genomic DNA. Translation: ACD95944.1.
RefSeq	YP_001952464.1. NC_010814.1.
3D structure databases
ProteinModelPortal	B3E4K8.
ModBase	Search...
Protein-protein interaction databases
STRING	B3E4K8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6368890.
GenomeReviews	Gene locus Glov_2228 in contig CP001089_GR.
KEGG	glo:Glov_2228.
PATRIC	21996137. VBIGeoLov31523_2164.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG351567.
OMA	YDISARD.
ProtClustDB	PRK13980.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_GEOLS

Accession

Primary (citable) accession number: B3E4K8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 22, 2008
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program