B3E467 (KDSA_GEOLS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-dehydro-3-deoxyphosphooctonate aldolase
EC=2.5.1.55

Alternative name(s):
3-deoxy-D-manno-octulosonic acid 8-phosphate synthase
KDO-8-phosphate synthase
Short name=KDO 8-P synthase
Short name=KDOPS
Phospho-2-dehydro-3-deoxyoctonate aldolase

Gene names

Name:	kdsA
Ordered Locus Names:	Glov_2172

Organism

Geobacter lovleyi (strain ATCC BAA-1151 / DSM 17278 / SZ) [Complete proteome] [HAMAP]

Taxonomic identifier

398767 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfuromonadales › Geobacteraceae › Geobacter

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + D-arabinose 5-phosphate + H2O = 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate. HAMAP MF_00056
Pathway	Carbohydrate biosynthesis; 3-deoxy-D-manno-octulosonate biosynthesis; 3-deoxy-D-manno-octulosonate from D-ribulose 5-phosphate: step 2/3. HAMAP MF_00056 Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis. HAMAP MF_00056
Subcellular location	Cytoplasm By similarity HAMAP MF_00056.
Sequence similarities	Belongs to the KdsA family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-deoxy-8-phosphooctulonate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 272	272	2-dehydro-3-deoxyphosphooctonate aldolase HAMAP MF_00056		PRO_1000091817

Sequences

	Sequence		Length	Mass (Da)
	B3E467 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 1EEE7AFED7FB6E95	FASTA	272	29,077
	10 20 30 40 50 60 MTKELSIGSV KMGGGRLLVL IAGPCVIESE EATLRHAERL MTICNGLGMP LIFKSSYDKA 70 80 90 100 110 120 NRTSINAFRG PGMDEGLRIL SKVKESLGIP VLSDIHSIEQ VAPAAQVLDV LQIPAFLCRQ 130 140 150 160 170 180 TDLVVAAAKT GKVVNVKKGQ FLAPWDMRNV VGKVAASGNE NIILTERGAS FGYNNLVVDM 190 200 210 220 230 240 RSFPVMRSYG YPVVFDATHS VQLPGGQGES SGGQREFVET LSRAAVATGI DGIFMEVHED 250 260 270 PSCALCDGPN SIPLAELPAL LKRLQALDAV VR

References

[1]

"Complete sequence of chromosome of Geobacter lovleyi SZ." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Meincke L., Brettin T., Detter J.C., Han C., Tapia R., Kuske C.R., Schmutz J., Larimer F. , Land M., Hauser L., Kyrpides N., Mikhailova N., Sung Y., Fletcher K.E., Ritalahti K.M., Loeffler F.E., Richardson P. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-1151 / DSM 17278 / SZ.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001089 Genomic DNA. Translation: ACD95888.1.
RefSeq	YP_001952408.1. NC_010814.1.
3D structure databases
ProteinModelPortal	B3E467.
ModBase	Search...
Protein-protein interaction databases
STRING	B3E467.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6367470.
GenomeReviews	Gene locus Glov_2172 in contig CP001089_GR.
KEGG	glo:Glov_2172.
PATRIC	21996027. VBIGeoLov31523_2111.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG575990.
OMA	CAKLNIP.
ProtClustDB	PRK05198.
Family and domain databases
HAMAP	MF_00056. KDO8P_synth. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR006218. DAHP1/KDSA. IPR006269. KDO8P_synthase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01627.
PANTHER	PTHR21057:SF2. KDO8P_synthase. 1 hit.
Pfam	PF00793. DAHP_synth_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01362. KDO8P_synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KDSA_GEOLS

Accession

Primary (citable) accession number: B3E467

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 22, 2008
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program