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B3E262 (B3E262_GEOLS) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoenolpyruvate carboxykinase [GTP] HAMAP-Rule MF_00452

Short name=PEP carboxykinase HAMAP-Rule MF_00452
Short name=PEPCK HAMAP-Rule MF_00452
EC=4.1.1.32 HAMAP-Rule MF_00452
Alternative name(s):
Phosphoenolpyruvate carboxylase HAMAP-Rule MF_00452
Gene names
Name:pckG HAMAP-Rule MF_00452
Ordered Locus Names:Glov_3462 EMBL ACD97165.1
OrganismGeobacter lovleyi (strain ATCC BAA-1151 / DSM 17278 / SZ) [Complete proteome] [HAMAP] EMBL ACD97165.1
Taxonomic identifier398767 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesGeobacteraceaeGeobacter

Protein attributes

Sequence length616 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity. HAMAP-Rule MF_00452

Catalytic activity

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2. HAMAP-Rule MF_00452

Cofactor

Binds 1 manganese ion per subunit By similarity. HAMAP-Rule MF_00452

Pathway

Carbohydrate biosynthesis; gluconeogenesis. HAMAP-Rule MF_00452

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00452

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00452.

Sequence similarities

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family. HAMAP-Rule MF_00452

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding262 – 2676GTP By similarity HAMAP-Rule MF_00452
Nucleotide binding506 – 5094GTP By similarity HAMAP-Rule MF_00452
Region380 – 3823Substrate binding By similarity HAMAP-Rule MF_00452

Sites

Active site2631 By similarity HAMAP-Rule MF_00452
Metal binding2191Manganese By similarity HAMAP-Rule MF_00452
Metal binding2391Manganese; via tele nitrogen By similarity HAMAP-Rule MF_00452
Metal binding2901Manganese By similarity HAMAP-Rule MF_00452
Binding site721Substrate By similarity HAMAP-Rule MF_00452
Binding site2121Substrate; via amide nitrogen By similarity HAMAP-Rule MF_00452
Binding site2191Substrate By similarity HAMAP-Rule MF_00452
Binding site2611Substrate By similarity HAMAP-Rule MF_00452
Binding site3821GTP By similarity HAMAP-Rule MF_00452
Binding site4131GTP By similarity HAMAP-Rule MF_00452

Sequences

Sequence LengthMass (Da)Tools
B3E262 [UniParc].

Last modified July 22, 2008. Version 1.
Checksum: 63C63D9F8456221F

FASTA61669,354
        10         20         30         40         50         60 
MSYEVKNEQL KAWVAEVEAM CEPANIHWCD GSQEEYDRLC GQMVESGTFR KLNPEKRPNS 

        70         80         90        100        110        120 
YLAFSDPIDV ARVEDRTFIC SLNKQDAGPT NNWVAPKEMK ETLKGLFKGC MKGRTMYVIP 

       130        140        150        160        170        180 
FSMGPLGSNI AKIGVEITDS PYVVVNMRIM TRMGKQVLDV LGNGEFVPCL HSVGMPLAEG 

       190        200        210        220        230        240 
QKDVAWPCNK EKYIVHFPEE RAIWSFGSGY GGNALLGKKC LALRIASVQA RDEGWLAEHM 

       250        260        270        280        290        300 
LILGVESPTG EKSYVGAAFP SACGKTNFAM LIPPKPFQDK GWKVTTIGDD IAWIKPGADG 

       310        320        330        340        350        360 
QVYAINPEYG YFGVAPGTNY KTNPNAMISC EKNSIFTNVA LTEDGDVWWE GMDGEVPAKL 

       370        380        390        400        410        420 
TDWQGNAWTP DCGRPAAHPN ARFTAPASQC PCIDPEWENP KGVPMSAFIF GGRRKDTVPL 

       430        440        450        460        470        480 
VYQAFNWNYG VYLASTLGSE TTAAAVGATG NVRRDPFAML PFCGYHMADY FAHWLQFGRS 

       490        500        510        520        530        540 
IARPPRIFSV NWFRKDANGK FIWPGYGENM RVLKWIAERV QGKTGSVECP LGWMPRYEDM 

       550        560        570        580        590        600 
DWEGIEMSRE KFNELMSVDR DVWQNEVISH EELFVKMYDK LPKEFLHIRE LILSSLWRSP 

       610 
EHWEQIGEVH PEGWNE 

« Hide

References

[1]"Complete sequence of chromosome of Geobacter lovleyi SZ."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Meincke L., Brettin T., Detter J.C., Han C., Tapia R., Kuske C.R., Schmutz J., Larimer F. expand/collapse author list , Land M., Hauser L., Kyrpides N., Mikhailova N., Sung Y., Fletcher K.E., Ritalahti K.M., Loeffler F.E., Richardson P.
Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-1151 / DSM 17278 / SZ.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001089 Genomic DNA. Translation: ACD97165.1.
RefSeqYP_001953685.1. NC_010814.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING398767.Glov_3462.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACD97165; ACD97165; Glov_3462.
GeneID6368947.
KEGGglo:Glov_3462.
PATRIC21998591. VBIGeoLov31523_3384.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1274.
HOGENOMHOG000191700.
KOK01596.
OMAWDNPAGV.
OrthoDBEOG6MPWQS.

Enzyme and pathway databases

BioCycGLOV398767:GH32-3514-MONOMER.
UniPathwayUPA00138.

Family and domain databases

Gene3D3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPMF_00452. PEPCK_GTP.
InterProIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERPTHR11561. PTHR11561. 1 hit.
PfamPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMSSF68923. SSF68923. 1 hit.
PROSITEPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB3E262_GEOLS
AccessionPrimary (citable) accession number: B3E262
Entry history
Integrated into UniProtKB/TrEMBL: July 22, 2008
Last sequence update: July 22, 2008
Last modified: June 11, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)