Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Geobacter lovleyi (strain ATCC BAA-1151 / DSM 17278 / SZ)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei72 – 721SubstrateUniRule annotation
Binding sitei212 – 2121Substrate; via amide nitrogenUniRule annotation
Metal bindingi219 – 2191ManganeseUniRule annotation
Binding sitei219 – 2191SubstrateUniRule annotation
Metal bindingi239 – 2391Manganese; via tele nitrogenUniRule annotation
Binding sitei261 – 2611SubstrateUniRule annotation
Active sitei263 – 2631UniRule annotation
Metal bindingi290 – 2901ManganeseUniRule annotation
Binding sitei382 – 3821GTPUniRule annotation
Binding sitei413 – 4131GTPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi262 – 2676GTPUniRule annotation
Nucleotide bindingi506 – 5094GTPUniRule annotation

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. kinase activity Source: UniProtKB-KW
  3. manganese ion binding Source: UniProtKB-HAMAP
  4. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

DecarboxylaseUniRule annotationSAAS annotation, KinaseImported, Lyase, Transferase

Keywords - Biological processi

GluconeogenesisUniRule annotation

Keywords - Ligandi

GTP-bindingUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding, PyruvateImported

Enzyme and pathway databases

BioCyciGLOV398767:GH32-3514-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotationSAAS annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:Glov_3462Imported
OrganismiGeobacter lovleyi (strain ATCC BAA-1151 / DSM 17278 / SZ)Imported
Taxonomic identifieri398767 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesGeobacteraceaeGeobacter
ProteomesiUP000002420 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi398767.Glov_3462.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni380 – 3823Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWMRFGED.
OrthoDBiEOG6MPWQS.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B3E262-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYEVKNEQL KAWVAEVEAM CEPANIHWCD GSQEEYDRLC GQMVESGTFR
60 70 80 90 100
KLNPEKRPNS YLAFSDPIDV ARVEDRTFIC SLNKQDAGPT NNWVAPKEMK
110 120 130 140 150
ETLKGLFKGC MKGRTMYVIP FSMGPLGSNI AKIGVEITDS PYVVVNMRIM
160 170 180 190 200
TRMGKQVLDV LGNGEFVPCL HSVGMPLAEG QKDVAWPCNK EKYIVHFPEE
210 220 230 240 250
RAIWSFGSGY GGNALLGKKC LALRIASVQA RDEGWLAEHM LILGVESPTG
260 270 280 290 300
EKSYVGAAFP SACGKTNFAM LIPPKPFQDK GWKVTTIGDD IAWIKPGADG
310 320 330 340 350
QVYAINPEYG YFGVAPGTNY KTNPNAMISC EKNSIFTNVA LTEDGDVWWE
360 370 380 390 400
GMDGEVPAKL TDWQGNAWTP DCGRPAAHPN ARFTAPASQC PCIDPEWENP
410 420 430 440 450
KGVPMSAFIF GGRRKDTVPL VYQAFNWNYG VYLASTLGSE TTAAAVGATG
460 470 480 490 500
NVRRDPFAML PFCGYHMADY FAHWLQFGRS IARPPRIFSV NWFRKDANGK
510 520 530 540 550
FIWPGYGENM RVLKWIAERV QGKTGSVECP LGWMPRYEDM DWEGIEMSRE
560 570 580 590 600
KFNELMSVDR DVWQNEVISH EELFVKMYDK LPKEFLHIRE LILSSLWRSP
610
EHWEQIGEVH PEGWNE
Length:616
Mass (Da):69,354
Last modified:July 22, 2008 - v1
Checksum:i63C63D9F8456221F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001089 Genomic DNA. Translation: ACD97165.1.
RefSeqiYP_001953685.1. NC_010814.1.

Genome annotation databases

EnsemblBacteriaiACD97165; ACD97165; Glov_3462.
KEGGiglo:Glov_3462.
PATRICi21998591. VBIGeoLov31523_3384.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001089 Genomic DNA. Translation: ACD97165.1.
RefSeqiYP_001953685.1. NC_010814.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi398767.Glov_3462.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACD97165; ACD97165; Glov_3462.
KEGGiglo:Glov_3462.
PATRICi21998591. VBIGeoLov31523_3384.

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWMRFGED.
OrthoDBiEOG6MPWQS.

Enzyme and pathway databases

UniPathwayiUPA00138.
BioCyciGLOV398767:GH32-3514-MONOMER.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-1151 / DSM 17278 / SZImported.

Entry informationi

Entry nameiB3E262_GEOLS
AccessioniPrimary (citable) accession number: B3E262
Entry historyi
Integrated into UniProtKB/TrEMBL: July 22, 2008
Last sequence update: July 22, 2008
Last modified: April 29, 2015
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.