B3E156 (EFTU_METI4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor Tu
Short name=EF-Tu

Gene names

Name:	tuf
Ordered Locus Names:	Minf_0797

Organism

Methylacidiphilum infernorum (isolate V4) (Methylokorus infernorum (strain V4)) [Complete proteome] [HAMAP]

Taxonomic identifier

481448 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Chlamydiae/Verrucomicrobia group › Verrucomicrobia › unclassified Verrucomicrobia › Methylacidiphilales › Methylacidiphilaceae › Methylacidiphilum › Methylacidiphilum infernorum

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis By similarity. HAMAP-Rule MF_00118
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00118
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00118.
Sequence similarities	Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Elongation factor
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: HAMAP GTPase activity Inferred from electronic annotation. Source: InterPro translation elongation factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 394	394	Elongation factor Tu HAMAP-Rule MF_00118		PRO_1000095076
Regions
	Nucleotide binding	19 – 26	8	GTP By similarity
	Nucleotide binding	81 – 85	5	GTP By similarity
	Nucleotide binding	136 – 139	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3E156 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: FF829F0644E5EC1C	FASTA	394	43,855
	10 20 30 40 50 60 MAKEAFLRKK PHINVGTIGH VDHGKTTLTS AITYVLAKKG LAQKMAYDQI DKAPEEKERG 70 80 90 100 110 120 ITINTAHVEY ESDKRHYAHV DCPGHADYIK NMITGAAQMD GAILVVSAAD GPMPQTREHI 130 140 150 160 170 180 LLARQVGVPY IVVFLNKVDM VDDKELLELV ELEVRELLNQ YGFPGDKIPI IKGSALKALE 190 200 210 220 230 240 GDPEYEKNIL ELVEAMDNYI PIPERPKDQP FLMPIEDVFN IEGRGTVVTG RVERGTLKRM 250 260 270 280 290 300 EEVEIVGIRP TTKTVVTDIE MFRKTLDTAE AGDNVGLLLR GIKKDDVERG QVVAKPGTIT 310 320 330 340 350 360 PHHKFNAQVY VLKKEEGGRH TAFFNGYRPQ FFFRTTDVTG TVTLKEGVEM VMPGDNVEFM 370 380 390 VELISPIAME KSMRFAIREG GKTVGAGVVT EIIE

References

[1]

"Complete genome sequence of the extremely acidophilic methanotroph isolate V4, Methylacidiphilum infernorum, a representative of the bacterial phylum Verrucomicrobia." Hou S., Makarova K.S., Saw J.H., Senin P., Ly B.V., Zhou Z., Ren Y., Wang J., Galperin M.Y., Omelchenko M.V., Wolf Y.I., Yutin N., Koonin E.V., Stott M.B., Mountain B.W., Crowe M.A., Smirnova A.V., Dunfield P.F. , Feng L., Wang L., Alam M. Biol. Direct 3:26-26(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Isolate V4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000975 Genomic DNA. Translation: ACD82852.1.
RefSeq	YP_001939450.1. NC_010794.1.
3D structure databases
ProteinModelPortal	B3E156.
ModBase	Search...
Protein-protein interaction databases
STRING	481448.Minf_0797.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACD82852; ACD82852; Minf_0797.
GeneID	6351720.
KEGG	min:Minf_0797.
PATRIC	22490673. VBIMetInf111569_0834.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0050.
HOGENOM	HOG000229290.
KO	K02358.
OMA	CEFVGYN.
ProtClustDB	PRK00049.
Enzyme and pathway databases
BioCyc	MINF481448:GJEI-810-MONOMER.
Family and domain databases
HAMAP	MF_00118_B. EF_Tu_B.
InterPro	IPR000795. EF_GTP-bd_dom. IPR027417. P-loop_NTPase. IPR005225. Small_GTP-bd_dom. IPR009001. Transl_elong_EF1A/Init_IF2_C. IPR004161. Transl_elong_EFTu/EF1A_2. IPR004541. Transl_elong_EFTu/EF1A_bac/org. IPR004160. Transl_elong_EFTu/EF1A_C. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
Pfam	PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. PF03143. GTP_EFTU_D3. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SUPFAM	SSF50465. Elong_init_C. 1 hit. SSF52540. SSF52540. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00485. EF-Tu. 1 hit. TIGR00231. small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EFTU_METI4

Accession

Primary (citable) accession number: B3E156

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 22, 2008
Last modified:	May 29, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program