ID   B3E0X2_METI4            Unreviewed;       314 AA.
AC   B3E0X2;
DT   22-JUL-2008, integrated into UniProtKB/TrEMBL.
DT   22-JUL-2008, sequence version 1.
DT   27-MAR-2024, entry version 71.
DE   RecName: Full=dTDP-glucose 4,6-dehydratase {ECO:0000256|ARBA:ARBA00011990};
DE            EC=4.2.1.46 {ECO:0000256|ARBA:ARBA00011990};
GN   Name=rfbB {ECO:0000313|EMBL:ACD84449.1};
GN   OrderedLocusNames=Minf_2395 {ECO:0000313|EMBL:ACD84449.1};
OS   Methylacidiphilum infernorum (isolate V4) (Methylokorus infernorum (strain
OS   V4)).
OC   Bacteria; Verrucomicrobiota; Methylacidiphilae; Methylacidiphilales;
OC   Methylacidiphilaceae; Methylacidiphilum (ex Ratnadevi et al. 2023).
OX   NCBI_TaxID=481448 {ECO:0000313|EMBL:ACD84449.1, ECO:0000313|Proteomes:UP000009149};
RN   [1] {ECO:0000313|EMBL:ACD84449.1, ECO:0000313|Proteomes:UP000009149}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Isolate V4 {ECO:0000313|Proteomes:UP000009149};
RX   PubMed=18593465; DOI=10.1186/1745-6150-3-26;
RA   Hou S., Makarova K.S., Saw J.H., Senin P., Ly B.V., Zhou Z., Ren Y.,
RA   Wang J., Galperin M.Y., Omelchenko M.V., Wolf Y.I., Yutin N., Koonin E.V.,
RA   Stott M.B., Mountain B.W., Crowe M.A., Smirnova A.V., Dunfield P.F.,
RA   Feng L., Wang L., Alam M.;
RT   "Complete genome sequence of the extremely acidophilic methanotroph isolate
RT   V4, Methylacidiphilum infernorum, a representative of the bacterial phylum
RT   Verrucomicrobia.";
RL   Biol. Direct 3:26-26(2008).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=dTDP-alpha-D-glucose = dTDP-4-dehydro-6-deoxy-alpha-D-glucose
CC         + H2O; Xref=Rhea:RHEA:17221, ChEBI:CHEBI:15377, ChEBI:CHEBI:57477,
CC         ChEBI:CHEBI:57649; EC=4.2.1.46;
CC         Evidence={ECO:0000256|ARBA:ARBA00001539};
CC   -!- COFACTOR:
CC       Name=NAD(+); Xref=ChEBI:CHEBI:57540;
CC         Evidence={ECO:0000256|ARBA:ARBA00001911};
CC   -!- SIMILARITY: Belongs to the NAD(P)-dependent epimerase/dehydratase
CC       family. dTDP-glucose dehydratase subfamily.
CC       {ECO:0000256|ARBA:ARBA00008178}.
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DR   EMBL; CP000975; ACD84449.1; -; Genomic_DNA.
DR   AlphaFoldDB; B3E0X2; -.
DR   STRING; 481448.Minf_2395; -.
DR   KEGG; min:Minf_2395; -.
DR   eggNOG; COG1088; Bacteria.
DR   HOGENOM; CLU_007383_1_14_0; -.
DR   Proteomes; UP000009149; Chromosome.
DR   GO; GO:0008460; F:dTDP-glucose 4,6-dehydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009225; P:nucleotide-sugar metabolic process; IEA:InterPro.
DR   CDD; cd05246; dTDP_GD_SDR_e; 1.
DR   Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR   Gene3D; 3.90.25.10; UDP-galactose 4-epimerase, domain 1; 1.
DR   InterPro; IPR005888; dTDP_Gluc_deHydtase.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   NCBIfam; TIGR01181; dTDP_gluc_dehyt; 1.
DR   PANTHER; PTHR43000:SF7; DTDP-D-GLUCOSE 4,6-DEHYDRATASE; 1.
DR   PANTHER; PTHR43000; DTDP-D-GLUCOSE 4,6-DEHYDRATASE-RELATED; 1.
DR   Pfam; PF16363; GDP_Man_Dehyd; 1.
DR   SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE   3: Inferred from homology;
KW   NAD {ECO:0000256|ARBA:ARBA00023027}.
FT   DOMAIN          7..282
FT                   /note="NAD(P)-binding"
FT                   /evidence="ECO:0000259|Pfam:PF16363"
SQ   SEQUENCE   314 AA;  35626 MW;  4621A3C39DC23C0E CRC64;
     MAGLMGKKNF EFIQADISDQ KRMEQILFEE KIEAIVHFAA ESHVDRSIDD PGLFVQSNVV
     GTYKLLESAF VYFSSLSPEK KERFRFIHIS TDEVYGSIPM DGAPTKEGGL YAPSSPYSAS
     KAASDHFVAA YYKTYGLPAL ITHSSNNYGP RQHPEKMIPH MICNALEEKQ LPVYGQGLNV
     RDWIYVEDHC EGVWKLLLKG RPGEVYHIGK GGGISNIELV KKICALLDSF FPRSSGKSYA
     ELIRFVADRP GHDLRYALDV SKMKREIGWQ AQVDLDRGLE KTLFWYVENR DWVQSVMNKG
     YTLKRQGLGR TRSI
//