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Protein

Glycerol-3-phosphate dehydrogenase [NAD(P)+]

Gene

gpsA

Organism
Methylacidiphilum infernorum (isolate V4) (Methylokorus infernorum (strain V4))
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

sn-glycerol 3-phosphate + NAD(P)+ = glycerone phosphate + NAD(P)H.UniRule annotationSAAS annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei71 – 711NAD; via amide nitrogenUniRule annotation
Binding sitei71 – 711SubstrateUniRule annotation
Binding sitei103 – 1031NAD; via amide nitrogenUniRule annotation
Active sitei154 – 1541Proton acceptorUniRule annotation
Binding sitei218 – 2181NADUniRule annotation
Binding sitei244 – 2441NADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi7 – 126NADUniRule annotation

GO - Molecular functioni

  1. glycerol-3-phosphate dehydrogenase [NAD(P)+] activity Source: UniProtKB-HAMAP
  2. glycerol-3-phosphate dehydrogenase [NAD+] activity Source: UniProtKB-EC
  3. glycerol-3-phosphate dehydrogenase [NADP+] activity Source: UniProtKB-EC
  4. NAD binding Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. glycerol-3-phosphate biosynthetic process Source: UniProtKB-HAMAP
  3. glycerol-3-phosphate catabolic process Source: InterPro
  4. glycerophospholipid metabolic process Source: UniProtKB-UniPathway
  5. phospholipid biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotation

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesisUniRule annotationSAAS annotation, Phospholipid metabolism

Keywords - Ligandi

NADUniRule annotation

Enzyme and pathway databases

BioCyciMINF481448:GJEI-187-MONOMER.
UniPathwayiUPA00940.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate dehydrogenase [NAD(P)+]UniRule annotation (EC:1.1.1.94UniRule annotation)
Alternative name(s):
NAD(P)H-dependent glycerol-3-phosphate dehydrogenaseUniRule annotation
Gene namesi
Name:gpsAUniRule annotationImported
Ordered Locus Names:Minf_0182Imported
OrganismiMethylacidiphilum infernorum (isolate V4) (Methylokorus infernorum (strain V4))Imported
Taxonomic identifieri481448 [NCBI]
Taxonomic lineageiBacteriaVerrucomicrobiaunclassified VerrucomicrobiaMethylacidiphilalesMethylacidiphilaceaeMethylacidiphilum
ProteomesiUP000009149 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. glycerol-3-phosphate dehydrogenase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Protein-protein interaction databases

STRINGi481448.Minf_0182.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni218 – 2192Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0240.
HOGENOMiHOG000246853.
KOiK00057.
OMAiRITIYAR.
OrthoDBiEOG6JDWF4.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_00394. NAD_Glyc3P_dehydrog.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. DH_multihelical.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11728. PTHR11728. 1 hit.
PfamiPF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000114. Glycerol-3-P_dh. 1 hit.
SUPFAMiSSF48179. SSF48179. 1 hit.
PROSITEiPS00957. NAD_G3PDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B3DXK8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIGILGQGR WGITIKKLLE ENNYEVLGFH HTDTSWGAKL DCLCVALPVQ
60 70 80 90 100
HIRETLFRFP SPRCPVISLS KGLEIGTGKR ASEIIKDVWK DAIVGALSGP
110 120 130 140 150
NFSEEILAGL PAASVVAAEE VNLGKFFQNI FHSKRFRVYR STDLVGVELG
160 170 180 190 200
GALKNIYAIA GGLCYGLKLG ENAHASLLTR SLSEMTRLGT AAGGKTETFF
210 220 230 240 250
GLSGVGDLLL TASSMKSRNF RLGARLASGV SLNEATAMEP TVIEGLPTTI
260 270 280 290
SVYKSRLFAQ QKKPIADEIY RILYENKKIH ESVVDLLERR VGEED
Length:295
Mass (Da):32,168
Last modified:July 21, 2008 - v1
Checksum:i6744697D087F0AE4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000975 Genomic DNA. Translation: ACD82242.1.
RefSeqiYP_001938841.1. NC_010794.1.

Genome annotation databases

EnsemblBacteriaiACD82242; ACD82242; Minf_0182.
GeneIDi6351097.
KEGGimin:Minf_0182.
PATRICi22489359. VBIMetInf111569_0190.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000975 Genomic DNA. Translation: ACD82242.1.
RefSeqiYP_001938841.1. NC_010794.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi481448.Minf_0182.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACD82242; ACD82242; Minf_0182.
GeneIDi6351097.
KEGGimin:Minf_0182.
PATRICi22489359. VBIMetInf111569_0190.

Phylogenomic databases

eggNOGiCOG0240.
HOGENOMiHOG000246853.
KOiK00057.
OMAiRITIYAR.
OrthoDBiEOG6JDWF4.

Enzyme and pathway databases

UniPathwayiUPA00940.
BioCyciMINF481448:GJEI-187-MONOMER.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_00394. NAD_Glyc3P_dehydrog.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. DH_multihelical.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11728. PTHR11728. 1 hit.
PfamiPF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000114. Glycerol-3-P_dh. 1 hit.
SUPFAMiSSF48179. SSF48179. 1 hit.
PROSITEiPS00957. NAD_G3PDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of the extremely acidophilic methanotroph isolate V4, Methylacidiphilum infernorum, a representative of the bacterial phylum Verrucomicrobia."
    Hou S., Makarova K.S., Saw J.H., Senin P., Ly B.V., Zhou Z., Ren Y., Wang J., Galperin M.Y., Omelchenko M.V., Wolf Y.I., Yutin N., Koonin E.V., Stott M.B., Mountain B.W., Crowe M.A., Smirnova A.V., Dunfield P.F.
    , Feng L., Wang L., Alam M.
    Biol. Direct 3:26-26(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Isolate V4Imported.

Entry informationi

Entry nameiB3DXK8_METI4
AccessioniPrimary (citable) accession number: B3DXK8
Entry historyi
Integrated into UniProtKB/TrEMBL: July 21, 2008
Last sequence update: July 21, 2008
Last modified: March 31, 2015
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.