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B3DXK8 (B3DXK8_METI4) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Glycerol-3-phosphate dehydrogenase [NAD(P)+] HAMAP-Rule MF_00394

EC=1.1.1.94 HAMAP-Rule MF_00394
Alternative name(s):
NAD(P)H-dependent glycerol-3-phosphate dehydrogenase HAMAP-Rule MF_00394
Gene names
Name:gpsA HAMAP-Rule MF_00394 EMBL ACD82242.1
Ordered Locus Names:Minf_0182 EMBL ACD82242.1
OrganismMethylacidiphilum infernorum (isolate V4) (Methylokorus infernorum (strain V4)) [Complete proteome] [HAMAP] EMBL ACD82242.1
Taxonomic identifier481448 [NCBI]
Taxonomic lineageBacteriaVerrucomicrobiaunclassified VerrucomicrobiaMethylacidiphilalesMethylacidiphilaceaeMethylacidiphilum

Protein attributes

Sequence length295 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

sn-glycerol 3-phosphate + NAD(P)+ = glycerone phosphate + NAD(P)H. HAMAP-Rule MF_00394 RuleBase RU000439 SAAS SAAS011128

Pathway

Membrane lipid metabolism; glycerophospholipid metabolism. HAMAP-Rule MF_00394 SAAS SAAS011128

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00394 SAAS SAAS011128.

Sequence similarities

Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family. HAMAP-Rule MF_00394

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding7 – 126NAD By similarity HAMAP-Rule MF_00394
Region218 – 2192Substrate binding By similarity HAMAP-Rule MF_00394 PIRSR PIRSR000114-2

Sites

Active site1541Proton acceptor By similarity HAMAP-Rule MF_00394 PIRSR PIRSR000114-1
Binding site711NAD; via amide nitrogen By similarity HAMAP-Rule MF_00394
Binding site711Substrate By similarity HAMAP-Rule MF_00394 PIRSR PIRSR000114-2
Binding site1031NAD; via amide nitrogen By similarity HAMAP-Rule MF_00394
Binding site2181NAD By similarity HAMAP-Rule MF_00394
Binding site2441NAD By similarity HAMAP-Rule MF_00394

Sequences

Sequence LengthMass (Da)Tools
B3DXK8 [UniParc].

Last modified July 22, 2008. Version 1.
Checksum: 6744697D087F0AE4

FASTA29532,168
        10         20         30         40         50         60 
MKIGILGQGR WGITIKKLLE ENNYEVLGFH HTDTSWGAKL DCLCVALPVQ HIRETLFRFP 

        70         80         90        100        110        120 
SPRCPVISLS KGLEIGTGKR ASEIIKDVWK DAIVGALSGP NFSEEILAGL PAASVVAAEE 

       130        140        150        160        170        180 
VNLGKFFQNI FHSKRFRVYR STDLVGVELG GALKNIYAIA GGLCYGLKLG ENAHASLLTR 

       190        200        210        220        230        240 
SLSEMTRLGT AAGGKTETFF GLSGVGDLLL TASSMKSRNF RLGARLASGV SLNEATAMEP 

       250        260        270        280        290 
TVIEGLPTTI SVYKSRLFAQ QKKPIADEIY RILYENKKIH ESVVDLLERR VGEED 

« Hide

References

[1]"Complete genome sequence of the extremely acidophilic methanotroph isolate V4, Methylacidiphilum infernorum, a representative of the bacterial phylum Verrucomicrobia."
Hou S., Makarova K.S., Saw J.H., Senin P., Ly B.V., Zhou Z., Ren Y., Wang J., Galperin M.Y., Omelchenko M.V., Wolf Y.I., Yutin N., Koonin E.V., Stott M.B., Mountain B.W., Crowe M.A., Smirnova A.V., Dunfield P.F. expand/collapse author list , Feng L., Wang L., Alam M.
Biol. Direct 3:26-26(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Isolate V4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000975 Genomic DNA. Translation: ACD82242.1.
RefSeqYP_001938841.1. NC_010794.1.

3D structure databases

ProteinModelPortalB3DXK8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING481448.Minf_0182.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACD82242; ACD82242; Minf_0182.
GeneID6351097.
KEGGmin:Minf_0182.
PATRIC22489359. VBIMetInf111569_0190.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0240.
HOGENOMHOG000246853.
KOK00057.
OMARITIYAR.
OrthoDBEOG6JDWF4.

Enzyme and pathway databases

BioCycMINF481448:GJEI-187-MONOMER.
UniPathwayUPA00940.

Family and domain databases

Gene3D1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPMF_00394. NAD_Glyc3P_dehydrog.
InterProIPR008927. 6-PGluconate_DH_C-like.
IPR013328. DH_multihelical.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11728. PTHR11728. 1 hit.
PfamPF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view]
PIRSFPIRSF000114. Glycerol-3-P_dh. 1 hit.
SUPFAMSSF48179. SSF48179. 1 hit.
PROSITEPS00957. NAD_G3PDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB3DXK8_METI4
AccessionPrimary (citable) accession number: B3DXK8
Entry history
Integrated into UniProtKB/TrEMBL: July 22, 2008
Last sequence update: July 22, 2008
Last modified: February 19, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)