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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Methylacidiphilum infernorum (isolate V4) (Methylokorus infernorum (strain V4))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei216ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMINF481448:G1GBH-159-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:Minf_0178
OrganismiMethylacidiphilum infernorum (isolate V4) (Methylokorus infernorum (strain V4))
Taxonomic identifieri481448 [NCBI]
Taxonomic lineageiBacteriaVerrucomicrobiaMethylacidiphilaeMethylacidiphilalesMethylacidiphilaceaeMethylacidiphilum
Proteomesi
  • UP000009149 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003677071 – 444Glutamate--tRNA ligaseAdd BLAST444

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi481448.Minf_0178

Structurei

3D structure databases

ProteinModelPortaliB3DXK4
SMRiB3DXK4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi12 – 22"HIGH" regionUniRule annotationAdd BLAST11
Motifi213 – 217"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252722
KOiK01885
OMAiHYINTLP
OrthoDBiPOG091H021W

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
3.40.50.620, 2 hits
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 2 hits
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

B3DXK4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKMIPRVRF APSPTGFLHV GGARTALFNW LYARHFKGTF ILRIEDTDQS
60 70 80 90 100
RNTPQALSVI FDNLSWLGLD WDEGPLPDGS SKGQFGPYFQ SQRKEIYNEY
110 120 130 140 150
CNRLIAKELA YIKDEAVYFR MPRKRIIVSD LICGDIYFDC SLEKDFVIRR
160 170 180 190 200
KDGSFVFHLV NVVDDLEMQI SHVIRGEDHL SNTPKHIALF EALGMSPPLY
210 220 230 240 250
AHIPLILNPS GTKMSKRDKG SSVQEYIDEG FLPQAFRNYL CLLGWSLKEN
260 270 280 290 300
REIFGIEEAI AKFDLPQIHR SNARFNHQKL LWINGEYMRS LPLDELYPHA
310 320 330 340 350
FFWLKKAGLI DQNTDCSFLK QAVGIVREKV KTGKELVEWM KPLLSDTLQY
360 370 380 390 400
DEAVVQQYLD DKGKEILREA LPYLEEVSSF QAKELEAIIK NLSLKMGRKT
410 420 430 440
ADYIHRLRVA LTGRTVGPSL YPMLEVLGKQ KVLNRLYKVV GPKE
Length:444
Mass (Da):51,186
Last modified:July 22, 2008 - v1
Checksum:iB607773531D0C967
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000975 Genomic DNA Translation: ACD82238.1
RefSeqiWP_012462520.1, NC_010794.1

Genome annotation databases

EnsemblBacteriaiACD82238; ACD82238; Minf_0178
KEGGimin:Minf_0178

Similar proteinsi

Entry informationi

Entry nameiSYE_METI4
AccessioniPrimary (citable) accession number: B3DXK4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 22, 2008
Last modified: March 28, 2018
This is version 60 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health