B3DXK4 (SYE_METI4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	Minf_0178

Organism

Methylacidiphilum infernorum (isolate V4) (Methylokorus infernorum (strain V4)) [Complete proteome] [HAMAP]

Taxonomic identifier

481448 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Chlamydiae/Verrucomicrobia group › Verrucomicrobia › unclassified Verrucomicrobia › Methylacidiphilales › Methylacidiphilaceae › Methylacidiphilum › Methylacidiphilum infernorum

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00022
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00022.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 444	444	Glutamate--tRNA ligase HAMAP-Rule MF_00022		PRO_0000367707
Regions
	Motif	12 – 22	11	"HIGH" region HAMAP-Rule MF_00022
	Motif	213 – 217	5	"KMSKS" region HAMAP-Rule MF_00022
Sites
	Binding site	216	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3DXK4 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: B607773531D0C967	FASTA	444	51,186
	10 20 30 40 50 60 MNKMIPRVRF APSPTGFLHV GGARTALFNW LYARHFKGTF ILRIEDTDQS RNTPQALSVI 70 80 90 100 110 120 FDNLSWLGLD WDEGPLPDGS SKGQFGPYFQ SQRKEIYNEY CNRLIAKELA YIKDEAVYFR 130 140 150 160 170 180 MPRKRIIVSD LICGDIYFDC SLEKDFVIRR KDGSFVFHLV NVVDDLEMQI SHVIRGEDHL 190 200 210 220 230 240 SNTPKHIALF EALGMSPPLY AHIPLILNPS GTKMSKRDKG SSVQEYIDEG FLPQAFRNYL 250 260 270 280 290 300 CLLGWSLKEN REIFGIEEAI AKFDLPQIHR SNARFNHQKL LWINGEYMRS LPLDELYPHA 310 320 330 340 350 360 FFWLKKAGLI DQNTDCSFLK QAVGIVREKV KTGKELVEWM KPLLSDTLQY DEAVVQQYLD 370 380 390 400 410 420 DKGKEILREA LPYLEEVSSF QAKELEAIIK NLSLKMGRKT ADYIHRLRVA LTGRTVGPSL 430 440 YPMLEVLGKQ KVLNRLYKVV GPKE

References

[1]

"Complete genome sequence of the extremely acidophilic methanotroph isolate V4, Methylacidiphilum infernorum, a representative of the bacterial phylum Verrucomicrobia." Hou S., Makarova K.S., Saw J.H., Senin P., Ly B.V., Zhou Z., Ren Y., Wang J., Galperin M.Y., Omelchenko M.V., Wolf Y.I., Yutin N., Koonin E.V., Stott M.B., Mountain B.W., Crowe M.A., Smirnova A.V., Dunfield P.F. , Feng L., Wang L., Alam M. Biol. Direct 3:26-26(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Isolate V4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000975 Genomic DNA. Translation: ACD82238.1.
RefSeq	YP_001938837.1. NC_010794.1.
3D structure databases
ProteinModelPortal	B3DXK4.
ModBase	Search...
Protein-protein interaction databases
STRING	481448.Minf_0178.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACD82238; ACD82238; Minf_0178.
GeneID	6351093.
KEGG	min:Minf_0178.
PATRIC	22489349. VBIMetInf111569_0185.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HOG000252722.
KO	K01885.
OMA	FKISEQE.
Enzyme and pathway databases
BioCyc	MINF481448:GJEI-183-MONOMER.
Family and domain databases
Gene3D	1.10.10.350. 1 hit. 1.10.1160.10. 1 hit. 3.40.50.620. 2 hits.
HAMAP	MF_00022_B. Glu_tRNA_synth_B.
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-ligase_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR10119. PTHR10119. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 2 hits. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_METI4

Accession

Primary (citable) accession number: B3DXK4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 22, 2008
Last modified:	May 1, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program