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B3DVG5 (RBL_METI4) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribulose bisphosphate carboxylase large chain

Short name=RuBisCO large subunit
EC=4.1.1.39
Gene names
Name:cbbL
Synonyms:rbcL
Ordered Locus Names:Minf_1264
OrganismMethylacidiphilum infernorum (isolate V4) (Methylokorus infernorum (strain V4)) [Complete proteome] [HAMAP]
Taxonomic identifier481448 [NCBI]
Taxonomic lineageBacteriaVerrucomicrobiaunclassified VerrucomicrobiaMethylacidiphilalesMethylacidiphilaceaeMethylacidiphilum

Protein attributes

Sequence length486 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site By similarity. HAMAP-Rule MF_01338

Catalytic activity

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O. HAMAP-Rule MF_01338

3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2. HAMAP-Rule MF_01338

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_01338

Subunit structure

Heterohexadecamer of 8 large chains and 8 small chains By similarity.

Miscellaneous

The basic functional RuBisCO is composed of a large chain homodimer in a "head-to-tail" conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric "cap" on each end of the "barrel" By similarity.

Sequence similarities

Belongs to the RuBisCO large chain family. Type I subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 486486Ribulose bisphosphate carboxylase large chain HAMAP-Rule MF_01338
PRO_0000355750

Sites

Active site1781Proton acceptor By similarity
Active site2961Proton acceptor By similarity
Metal binding2041Magnesium; via carbamate group By similarity
Metal binding2061Magnesium By similarity
Metal binding2071Magnesium By similarity
Binding site1261Substrate; in homodimeric partner By similarity
Binding site1761Substrate By similarity
Binding site1801Substrate By similarity
Binding site2971Substrate By similarity
Binding site3291Substrate By similarity
Binding site3811Substrate By similarity
Site3361Transition state stabilizer By similarity

Amino acid modifications

Modified residue2041N6-carboxylysine By similarity

Sequences

Sequence LengthMass (Da)Tools
B3DVG5 [UniParc].

Last modified July 22, 2008. Version 1.
Checksum: A0A9CE650CA08146

FASTA48653,694
        10         20         30         40         50         60 
MVIAGDGKAG AKKSRWSAGV TPYAEMGYYN ADYVPKDTDI LAAFRFVPQE GVEPIEAGAA 

        70         80         90        100        110        120 
VAGESSTATW TVVWTDRLTA YEHYQGKCFR VEPVPGTNQY IAFIAYDLDL FEEGSIANMS 

       130        140        150        160        170        180 
SSIIGNVFGF KALKSLRLED LRIPPHYVKT FQGPAHGIMM EREYLNKYGR PLLGATVKPK 

       190        200        210        220        230        240 
LGLSAKNYGR VVYEALRGGL DFTKDDENIN SQPFMRWRDR WLFCMEAVNK AMAETGEIKG 

       250        260        270        280        290        300 
HYLNVTAATM EEMYERAEFA KELGSVIIMV DLTAGFTAIQ SMAKWCRKNG VLLHLHRAGH 

       310        320        330        340        350        360 
STYTRQKIHG VNFRVIAKWM RLAGVDHIHA GTVVGKLEGD LHSVQGYYKT LRTQYTEADP 

       370        380        390        400        410        420 
LLGLYFEQDW ASMPGVMPVA SGGIHAGQMH LLLSYLGEDT ILQFGGGTIG HPDGIAAGAT 

       430        440        450        460        470        480 
ANRVAVEVMV QARNEGKDIL REGPEILEKA CRWSPALAKA IETWKDISFE FESTDVPDAV 


AMPTIA 

« Hide

References

[1]"Complete genome sequence of the extremely acidophilic methanotroph isolate V4, Methylacidiphilum infernorum, a representative of the bacterial phylum Verrucomicrobia."
Hou S., Makarova K.S., Saw J.H., Senin P., Ly B.V., Zhou Z., Ren Y., Wang J., Galperin M.Y., Omelchenko M.V., Wolf Y.I., Yutin N., Koonin E.V., Stott M.B., Mountain B.W., Crowe M.A., Smirnova A.V., Dunfield P.F. expand/collapse author list , Feng L., Wang L., Alam M.
Biol. Direct 3:26-26(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Isolate V4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000975 Genomic DNA. Translation: ACD83318.1.
RefSeqYP_001939916.1. NC_010794.1.

3D structure databases

ProteinModelPortalB3DVG5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING481448.Minf_1264.

Proteomic databases

PRIDEB3DVG5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACD83318; ACD83318; Minf_1264.
GeneID6352199.
KEGGmin:Minf_1264.
PATRIC22491667. VBIMetInf111569_1320.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1850.
HOGENOMHOG000230831.
KOK01601.
OMACTPLKQA.
OrthoDBEOG6ZKXMS.

Enzyme and pathway databases

BioCycMINF481448:GJEI-1289-MONOMER.

Family and domain databases

Gene3D3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPMF_01338. RuBisCO_L_type1.
InterProIPR020878. RuBisCo_large_chain_AS.
IPR020888. RuBisCO_lsu.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view]
PfamPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
PROSITEPS00157. RUBISCO_LARGE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRBL_METI4
AccessionPrimary (citable) accession number: B3DVG5
Entry history
Integrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: July 22, 2008
Last modified: May 14, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families