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B3DV36

- BIOB_METI4

UniProt

B3DV36 - BIOB_METI4

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Protein
Biotin synthase
Gene
bioB, Minf_1134
Organism
Methylacidiphilum infernorum (isolate V4) (Methylokorus infernorum (strain V4))
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi47 – 471Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi51 – 511Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi54 – 541Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi91 – 911Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi124 – 1241Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi184 – 1841Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi256 – 2561Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMINF481448:GJEI-1158-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:Minf_1134
OrganismiMethylacidiphilum infernorum (isolate V4) (Methylokorus infernorum (strain V4))
Taxonomic identifieri481448 [NCBI]
Taxonomic lineageiBacteriaVerrucomicrobiaunclassified VerrucomicrobiaMethylacidiphilalesMethylacidiphilaceaeMethylacidiphilum
ProteomesiUP000009149: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 311311Biotin synthaseUniRule annotation
PRO_0000381462Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi481448.Minf_1134.

Structurei

3D structure databases

ProteinModelPortaliB3DV36.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

B3DV36-1 [UniParc]FASTAAdd to Basket

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MKDYCAIEQI YHRPLDELLG EALQAKKLSG RNGVQFCQLL NIKSGGCSED    50
CKYCAQSAHY RTPIEKGALL DEEEILQAGL QAKEKGASRF CLGAAWRGLF 100
EGETKTKKIC KIISKISSLG MELCLSAGFL TEKTALMLKE SGLKVYNHNL 150
NTGPSYYPRI ASTHRFEDRL QTIRIVQKVG LKLCSGGIIG MGERLKDRLE 200
MLFCLYSLPE APESIPINVY MPIEGTPFYG TPPLDYMDLI RMIATTRILF 250
PLSRIRLAAG RKLLDEKTLT LCYLAGVDSI FIGEKLLTQS NVQLEKDYAL 300
LKKLNLRKEE R 311
Length:311
Mass (Da):34,942
Last modified:July 28, 2009 - v2
Checksum:i18E9986EEDB67694
GO

Sequence cautioni

The sequence ACD83189.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000975 Genomic DNA. Translation: ACD83189.1. Different initiation.
RefSeqiYP_001939787.1. NC_010794.1.

Genome annotation databases

EnsemblBacteriaiACD83189; ACD83189; Minf_1134.
GeneIDi6352068.
KEGGimin:Minf_1134.
PATRICi22491409. VBIMetInf111569_1192.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000975 Genomic DNA. Translation: ACD83189.1 . Different initiation.
RefSeqi YP_001939787.1. NC_010794.1.

3D structure databases

ProteinModelPortali B3DV36.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 481448.Minf_1134.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACD83189 ; ACD83189 ; Minf_1134 .
GeneIDi 6352068.
KEGGi min:Minf_1134.
PATRICi 22491409. VBIMetInf111569_1192.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci MINF481448:GJEI-1158-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of the extremely acidophilic methanotroph isolate V4, Methylacidiphilum infernorum, a representative of the bacterial phylum Verrucomicrobia."
    Hou S., Makarova K.S., Saw J.H., Senin P., Ly B.V., Zhou Z., Ren Y., Wang J., Galperin M.Y., Omelchenko M.V., Wolf Y.I., Yutin N., Koonin E.V., Stott M.B., Mountain B.W., Crowe M.A., Smirnova A.V., Dunfield P.F.
    , Feng L., Wang L., Alam M.
    Biol. Direct 3:26-26(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Isolate V4.

Entry informationi

Entry nameiBIOB_METI4
AccessioniPrimary (citable) accession number: B3DV36
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 28, 2009
Last modified: February 19, 2014
This is version 41 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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