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B3DV36 (BIOB_METI4) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein attributes

Sequence length311 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence caution

The sequence ACD83189.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 311311Biotin synthase HAMAP-Rule MF_01694
PRO_0000381462

Sites

Metal binding471Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding511Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding541Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding911Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1241Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1841Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2561Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
B3DV36 [UniParc].

Last modified July 28, 2009. Version 2.
Checksum: 18E9986EEDB67694

FASTA31134,942
        10         20         30         40         50         60 
MKDYCAIEQI YHRPLDELLG EALQAKKLSG RNGVQFCQLL NIKSGGCSED CKYCAQSAHY 

        70         80         90        100        110        120 
RTPIEKGALL DEEEILQAGL QAKEKGASRF CLGAAWRGLF EGETKTKKIC KIISKISSLG 

       130        140        150        160        170        180 
MELCLSAGFL TEKTALMLKE SGLKVYNHNL NTGPSYYPRI ASTHRFEDRL QTIRIVQKVG 

       190        200        210        220        230        240 
LKLCSGGIIG MGERLKDRLE MLFCLYSLPE APESIPINVY MPIEGTPFYG TPPLDYMDLI 

       250        260        270        280        290        300 
RMIATTRILF PLSRIRLAAG RKLLDEKTLT LCYLAGVDSI FIGEKLLTQS NVQLEKDYAL 

       310 
LKKLNLRKEE R 

« Hide

References

[1]"Complete genome sequence of the extremely acidophilic methanotroph isolate V4, Methylacidiphilum infernorum, a representative of the bacterial phylum Verrucomicrobia."
Hou S., Makarova K.S., Saw J.H., Senin P., Ly B.V., Zhou Z., Ren Y., Wang J., Galperin M.Y., Omelchenko M.V., Wolf Y.I., Yutin N., Koonin E.V., Stott M.B., Mountain B.W., Crowe M.A., Smirnova A.V., Dunfield P.F. expand/collapse author list , Feng L., Wang L., Alam M.
Biol. Direct 3:26-26(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Isolate V4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000975 Genomic DNA. Translation: ACD83189.1. Different initiation.
RefSeqYP_001939787.1. NC_010794.1.

3D structure databases

ProteinModelPortalB3DV36.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING481448.Minf_1134.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACD83189; ACD83189; Minf_1134.
GeneID6352068.
KEGGmin:Minf_1134.
PATRIC22491409. VBIMetInf111569_1192.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycMINF481448:GJEI-1158-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_METI4
AccessionPrimary (citable) accession number: B3DV36
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 28, 2009
Last modified: February 19, 2014
This is version 41 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways