B3DV19 (NTPA_METI4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase
EC=3.6.1.19

Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase

Gene names

Ordered Locus Names:

Minf_1117

Organism

Methylacidiphilum infernorum (isolate V4) (Methylokorus infernorum (strain V4)) [Complete proteome] [HAMAP]

Taxonomic identifier

481448 [NCBI]

Taxonomic lineage

Bacteria › Verrucomicrobia › unclassified Verrucomicrobia › Methylacidiphilales › Methylacidiphilaceae › Methylacidiphilum

Protein attributes

Sequence length	202 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP MF_01405
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.
Subunit structure	Homodimer By similarity. HAMAP MF_01405
Sequence similarities	Belongs to the HAM1 NTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 202	202	Non-canonical purine NTP pyrophosphatase HAMAP MF_01405		PRO_1000145494
Regions
	Region	10 – 15	6	Substrate binding By similarity
	Region	70 – 71	2	Substrate binding By similarity
Sites
	Metal binding	70	1	Magnesium or manganese By similarity
	Binding site	156	1	Substrate By similarity
	Binding site	176	1	Substrate By similarity
	Binding site	182	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3DV19 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: B627AD1D7209F431	FASTA	202	22,778
	10 20 30 40 50 60 MIMQKILLAT SNRHKYLEFS RLLYPLTLEA LPEDLKRLLP NETAKSYRDN AKLKGMALSE 70 80 90 100 110 120 IYEGFVLADD SGLEVFSLHG EPGIFSSRYA GEGSSAQENI DKLLKNLQSK NITDRRARFV 130 140 150 160 170 180 CALVLVKKKK ILFETTAFCY GIIADRQKGG GGFGYDPIFI PEGYSLTMAE LDEKQKDLVS 190 200 HRGKACQELK AFFLENRMEG HS

References

[1]

"Complete genome sequence of the extremely acidophilic methanotroph isolate V4, Methylacidiphilum infernorum, a representative of the bacterial phylum Verrucomicrobia." Hou S., Makarova K.S., Saw J.H., Senin P., Ly B.V., Zhou Z., Ren Y., Wang J., Galperin M.Y., Omelchenko M.V., Wolf Y.I., Yutin N., Koonin E.V., Stott M.B., Mountain B.W., Crowe M.A., Smirnova A.V., Dunfield P.F. , Feng L., Wang L., Alam M. Biol. Direct 3:26-26(2008) [PubMed: 18593465] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Isolate V4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000975 Genomic DNA. Translation: ACD83172.1.
RefSeq	YP_001939770.1. NC_010794.1.
3D structure databases
ProteinModelPortal	B3DV19.
ModBase	Search...
Protein-protein interaction databases
STRING	B3DV19.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6352050.
GenomeReviews	Gene locus Minf_1117 in contig CP000975_GR.
KEGG	min:Minf_1117.
PATRIC	22491379. VBIMetInf111569_1178.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG697237.
OMA	GVYTADW.
Family and domain databases
HAMAP	MF_01405. Non_canon_purine_NTPase. [Tree]
InterPro	IPR002637. Ham1p-like. IPR020922. Nucleoside-triphosphatase. [Graphical view]
KO	K02428.
PANTHER	PTHR11067. Ham1p_like. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
TIGRFAMs	TIGR00042. TIGR00042. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NTPA_METI4

Accession

Primary (citable) accession number: B3DV19

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 22, 2008
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program