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B3DU87

- SYE_BIFLD

UniProt

B3DU87 - SYE_BIFLD

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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Bifidobacterium longum (strain DJO10A)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei268 – 2681ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBLON205913:GJB4-1305-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:BLD_1261
OrganismiBifidobacterium longum (strain DJO10A)
Taxonomic identifieri205913 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeBifidobacterialesBifidobacteriaceaeBifidobacterium
ProteomesiUP000002419: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 506506Glutamate--tRNA ligasePRO_0000367617Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi205913.BLD_1261.

Structurei

3D structure databases

ProteinModelPortaliB3DU87.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi21 – 3111"HIGH" regionAdd
BLAST
Motifi265 – 2695"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK01885.
OMAiKHYDGDF.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

B3DU87-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTDAENTKPE LPKNVRVRFC PSPTGTPHVG MIRTALFNWA EARATGGTLI
60 70 80 90 100
FRIEDTDAVR DSEESYNQIL ESLRWLGIDW DEGIDVGGPH GPYRQSERTA
110 120 130 140 150
IYKDVAAKLL EAGYAYESFS TPEEIKERNL AAGRPAEFGY DGYDRNLTDE
160 170 180 190 200
QKAAFRAEGR KPALRIKMPD EDIAFDDLIR GTIEFKAGSV PDYVIVRPNG
210 220 230 240 250
DPLYTLTNPV DDAMMEVNVV LRGEDLLSST PRQIVLYRYL MELGIAKEMP
260 270 280 290 300
LFGHMPYVMG QGNKKLSKRD PESNLFNHRD NGFIREGLLN YLALLGWSIA
310 320 330 340 350
PDRDVFSMDE MTEKFDVRDV KANPARFDID KAISINAEHI RMLEPEDFLR
360 370 380 390 400
RSVPYLHRDG VVSADNWDAL TDREREVLTA AAPLVQPRVR LLGEVAGMVG
410 420 430 440 450
SLLSTEGYLE PADDAKKQLK DSAGEVLDKA IAALEAVDEA DWKTDNLHET
460 470 480 490 500
LNKALVEEGG YKPRLAFGPV RVAMSGRRVS PPLFESMEIV GKPVSLARLK

GLREHL
Length:506
Mass (Da):56,681
Last modified:July 22, 2008 - v1
Checksum:i3FB6166D424431AE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000605 Genomic DNA. Translation: ACD98706.1.
RefSeqiYP_001955204.1. NC_010816.1.

Genome annotation databases

EnsemblBacteriaiACD98706; ACD98706; BLD_1261.
GeneIDi6362885.
KEGGiblj:BLD_1261.
PATRICi21115510. VBIBifLon134794_1278.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000605 Genomic DNA. Translation: ACD98706.1 .
RefSeqi YP_001955204.1. NC_010816.1.

3D structure databases

ProteinModelPortali B3DU87.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 205913.BLD_1261.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACD98706 ; ACD98706 ; BLD_1261 .
GeneIDi 6362885.
KEGGi blj:BLD_1261.
PATRICi 21115510. VBIBifLon134794_1278.

Phylogenomic databases

eggNOGi COG0008.
HOGENOMi HOG000252720.
KOi K01885.
OMAi KHYDGDF.
OrthoDBi EOG6DRPF7.

Enzyme and pathway databases

BioCyci BLON205913:GJB4-1305-MONOMER.

Family and domain databases

Gene3Di 1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPi MF_00022_B. Glu_tRNA_synth_B.
InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
PANTHERi PTHR10119. PTHR10119. 1 hit.
Pfami PF00749. tRNA-synt_1c. 1 hit.
[Graphical view ]
PRINTSi PR00987. TRNASYNTHGLU.
SUPFAMi SSF48163. SSF48163. 1 hit.
TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Comparative genomic analysis of the gut bacterium Bifidobacterium longum reveals loci susceptible to deletion during pure culture growth."
    Lee J.H., Karamychev V.N., Kozyavkin S.A., Mills D., Pavlov A.R., Pavlova N.V., Polouchine N.N., Richardson P.M., Shakhova V.V., Slesarev A.I., Weimer B., O'Sullivan D.J.
    BMC Genomics 9:247-247(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DJO10A.

Entry informationi

Entry nameiSYE_BIFLD
AccessioniPrimary (citable) accession number: B3DU87
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 22, 2008
Last modified: October 29, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3