B3DTR2 (TRMD_BIFLD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(1)-)-methyltransferase
EC=2.1.1.228

Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	BLD_1085

Organism

Bifidobacterium longum (strain DJO10A) [Complete proteome] [HAMAP]

Taxonomic identifier

205913 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP MF_00605
Catalytic activity	S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA. HAMAP MF_00605
Subunit structure	Homodimer By similarity. HAMAP MF_00605
Subcellular location	Cytoplasm Potential HAMAP MF_00605.
Sequence similarities	Belongs to the RNA methyltransferase TrmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 288	288	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_1000130136
Regions
	Region	162 – 167	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	137	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3DTR2 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: A0728D39E9CF133C	FASTA	288	31,352
	10 20 30 40 50 60 MKIDIVSVFP EYFEVMNLSL MGKAQAKGLL EIKAHNLRDW THDVHHSVDD TPVGGGAGMV 70 80 90 100 110 120 MKPEVWSECL DELLGFSQPS AATDAVDTSD PGDSAAPDSS APSGAPVLIF PNPSAPLFTQ 130 140 150 160 170 180 RDATELSHAD HLLFGCGRYE GYDARIPDYY RAQGVDVREY SIGDYVLNGG EVAVSVMLEA 190 200 210 220 230 240 ITRLMPGFMG NPDSIVEESY TGEGALLEHR QYTKPAVWRG IAVPDVLLSG DHGKVDRFRR 250 260 270 280 DEALARTAEI RPDLIAALDC KALDKADRKT LMALGWEVSA AHPRRLAD

References

[1]

"Comparative genomic analysis of the gut bacterium Bifidobacterium longum reveals loci susceptible to deletion during pure culture growth." Lee J.H., Karamychev V.N., Kozyavkin S.A., Mills D., Pavlov A.R., Pavlova N.V., Polouchine N.N., Richardson P.M., Shakhova V.V., Slesarev A.I., Weimer B., O'Sullivan D.J. BMC Genomics 9:247-247(2008) [PubMed: 18505588] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DJO10A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000605 Genomic DNA. Translation: ACD98531.1.
RefSeq	YP_001955029.1. NC_010816.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B3DTR2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6364607.
GenomeReviews	Gene locus BLD_1085 in contig CP000605_GR.
KEGG	blj:BLD_1085.
PATRIC	21115134. VBIBifLon134794_1102.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285805.
OMA	VCGRFEG.
ProtClustDB	PRK00026.
Family and domain databases
HAMAP	MF_00605. TrmD. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. IPR023148. tRNA_m1G_MeTrfase_C. [Graphical view]
Gene3D	G3DSA:1.10.1270.20. tRNA_m1G_MeTrfase_C. 1 hit.
KO	K00554.
PANTHER	PTHR10056. PTHR10056. 1 hit.
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_BIFLD

Accession

Primary (citable) accession number: B3DTR2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 22, 2008
Last modified:	January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program