B3DTK7 (NTPA_BIFLD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase
EC=3.6.1.19

Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase

Gene names

Ordered Locus Names:

BLD_1030

Organism

Bifidobacterium longum (strain DJO10A) [Complete proteome] [HAMAP]

Taxonomic identifier

205913 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	252 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP MF_01405
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.
Subunit structure	Homodimer By similarity. HAMAP MF_01405
Sequence similarities	Belongs to the HAM1 NTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 252	252	Non-canonical purine NTP pyrophosphatase HAMAP MF_01405		PRO_1000145480
Regions
	Region	7 – 12	6	Substrate binding By similarity
	Region	74 – 75	2	Substrate binding By similarity
Sites
	Metal binding	74	1	Magnesium or manganese By similarity
	Binding site	196	1	Substrate By similarity
	Binding site	230	1	Substrate By similarity
	Binding site	236	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3DTK7 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 9AC7D82C052CE0E3	FASTA	252	26,448
	10 20 30 40 50 60 MQIVVATHNE GKLVEIRRIL EEDLGADAEN IELVSAGSLH LPDPVETGVT FQENALLKAR 70 80 90 100 110 120 AVAIRTGLPA VADDSGLIVD VMGNAPGILS ARWAGAHGHD KANNALLLAQ IEDIPDDKRT 130 140 150 160 170 180 ARFRCAAALV VPDTETGADV TGGVAADGIT VHTTAADGSP APVHARYAIK SETVELGDMP 190 200 210 220 230 240 GRIIREARGE HGFGYDPLFV PDDQPAGRVS TEPDHEGEPL TSAEMTPAEK NAISHRGKAL 250 KALVPAIEAL LH

References

[1]

"Comparative genomic analysis of the gut bacterium Bifidobacterium longum reveals loci susceptible to deletion during pure culture growth." Lee J.H., Karamychev V.N., Kozyavkin S.A., Mills D., Pavlov A.R., Pavlova N.V., Polouchine N.N., Richardson P.M., Shakhova V.V., Slesarev A.I., Weimer B., O'Sullivan D.J. BMC Genomics 9:247-247(2008) [PubMed: 18505588] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DJO10A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000605 Genomic DNA. Translation: ACD98476.1.
RefSeq	YP_001954974.1. NC_010816.1.
3D structure databases
ProteinModelPortal	B3DTK7.
ModBase	Search...
Protein-protein interaction databases
STRING	B3DTK7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6363318.
GenomeReviews	Gene locus BLD_1030 in contig CP000605_GR.
KEGG	blj:BLD_1030.
PATRIC	21115026. VBIBifLon134794_1048.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG697237.
OMA	GEAHNDA.
ProtClustDB	PRK00120.
Family and domain databases
HAMAP	MF_01405. Non_canon_purine_NTPase. [Tree]
InterPro	IPR002637. Ham1p-like. IPR020922. Nucleoside-triphosphatase. [Graphical view]
KO	K02428.
PANTHER	PTHR11067. Ham1p_like. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NTPA_BIFLD

Accession

Primary (citable) accession number: B3DTK7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 22, 2008
Last modified:	January 25, 2012
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program