B3DTI2 (B3DTI2_BIFLD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Beta-galactosidase PIRNR PIRNR001084
Short name=Beta-gal PIRNR PIRNR001084
EC=3.2.1.23 PIRNR PIRNR001084

Gene names

Name:	lacA2 EMBL ACD98451.1
Ordered Locus Names:	BLD_1005

Organism

Bifidobacterium longum (strain DJO10A) [Complete proteome] [HAMAP]

Taxonomic identifier

205913 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	710 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. PIRNR PIRNR001084
Sequence similarities	Belongs to the glycosyl hydrolase 42 family. PIRNR PIRNR001084

Ontologies

Keywords
Molecular function	Glycosidase PIRNR PIRNR001084 Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	beta-galactosidase complex Inferred from electronic annotation. Source: InterPro
Molecular function	beta-galactosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	161	1	Proton donor By similarity PIRSR PIRSR001084-1
	Active site	320	1	Nucleophile By similarity PIRSR PIRSR001084-1
	Binding site	122	1	Substrate By similarity PIRSR PIRSR001084-2
	Binding site	160	1	Substrate By similarity PIRSR PIRSR001084-2
	Binding site	328	1	Substrate By similarity PIRSR PIRSR001084-2

Sequences

	Sequence		Length	Mass (Da)
	B3DTI2 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: D5733D66AFCBEE63	FASTA	710	78,991
	10 20 30 40 50 60 MTTHRAFRWP SLLTESGRGI AFGGDYNPDQ WPEETLDEDI RLMGEAGVNV VSLAIFSWDK 70 80 90 100 110 120 IEPVEGAFTF EWLDHVIDRL GRAGIAVDLA SATAAAPLWL YESHPEVLPV DRYGHTVNAG 130 140 150 160 170 180 SRQSWQPTSP VFKEYALRLC RRLAEHYKDN PYVTAWHMGN EYGWNNRYDY SDNALAAFRT 190 200 210 220 230 240 WCEAKYGTID ALNEAWGTAF WSQHVNSFDE VLLPRHMGGD AMVNPSQQLD YERFGNDMLL 250 260 270 280 290 300 DFYKAERDAI EQICPDKPFT TNFMVSTDQC VMNYAKWADE VDFVSNDHYF HEGESHLDEL 310 320 330 340 350 360 ACSDALMDSL ALGKPWYVME HSTSAVQWKP LNTRKRAGEL MRDSLAHVAM GADAICFFQW 370 380 390 400 410 420 RQSKSGAEAF HSAMLPHAGA DSKVFRGVCE LGKALKTLSD AGLQGTELER AGTAILFSAE 430 440 450 460 470 480 SEWATRSETL PSMKLNHWHD VRDWYRGFLD AGLRADVVPL AYDWTGYKTI VLPTVLSLSD 490 500 510 520 530 540 EDVLRIADFA KAGGTVIVGY AAGLIDEHFH IGLGGYPGAG NGLLRDMLGI RSEEFNILGE 550 560 570 580 590 600 EAEGEPSEIS LSNGLTTRLW QNDVTSVAAD TTVLASYAGK SAADWELERT PAITSRPYGN 610 620 630 640 650 660 GTAIYVGCDL NRHDIAQLLK ALGSRWQELS AQPTESGQTP TYPTTDPRIL HTIRRSADGS 670 680 690 700 710 TRFDFYLNRS NQPVAINGVE GDPIIAHRCE TDAVGYTLNR NAILIAKTSC

References

[1]

"Comparative genomic analysis of the gut bacterium Bifidobacterium longum reveals loci susceptible to deletion during pure culture growth." Lee J.H., Karamychev V.N., Kozyavkin S.A., Mills D., Pavlov A.R., Pavlova N.V., Polouchine N.N., Richardson P.M., Shakhova V.V., Slesarev A.I., Weimer B., O'Sullivan D.J. BMC Genomics 9:247-247(2008) [PubMed: 18505588] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000605 Genomic DNA. Translation: ACD98451.1.
RefSeq	YP_001954949.1. NC_010816.1.
3D structure databases
ProteinModelPortal	B3DTI2.
ModBase	Search...
Protein-protein interaction databases
STRING	B3DTI2.
Protein family/group databases
CAZy	GH42. Glycoside Hydrolase Family 42.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6363535.
GenomeReviews	Gene locus BLD_1005 in contig CP000605_GR.
KEGG	blj:BLD_1005.
PATRIC	21114966. VBIBifLon134794_1021.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG476453.
OMA	ESEWATR.
ProtClustDB	CLSK572549.
Family and domain databases
InterPro	IPR013739. Beta_galactosidase_C. IPR013738. Beta_galactosidase_Trimer. IPR003476. Glyco_hydro_42. IPR013529. Glyco_hydro_42_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
KO	K12308.
Pfam	PF02449. Glyco_hydro_42. 1 hit. PF08533. Glyco_hydro_42C. 1 hit. PF08532. Glyco_hydro_42M. 1 hit. [Graphical view]
PIRSF	PIRSF001084. B-galactosidase. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B3DTI2_BIFLD

Accession

Primary (citable) accession number: B3DTI2

Entry history

Integrated into UniProtKB/TrEMBL:	July 22, 2008
Last sequence update:	July 22, 2008
Last modified:	January 25, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)