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B3DTF8 (LEU3_BIFLD) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 28. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
3-isopropylmalate dehydrogenase
EC=1.1.1.85
Alternative name(s):
3-IPM-DH
Beta-IPM dehydrogenase
Short name=IMDH
Gene names
Name:leuB
Ordered Locus Names:BLD_0981
OrganismBifidobacterium longum (strain DJO10A) [Complete proteome] [HAMAP]
Taxonomic identifier205913 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length343 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate By similarity. HAMAP MF_01035

Catalytic activity

(2R,3S)-3-isopropylmalate + NAD+ = 4-methyl-2-oxopentanoate + CO2 + NADH. HAMAP MF_01035

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4. HAMAP MF_01035

Subunit structure

Homodimer By similarity. HAMAP MF_01035

Subcellular location

Cytoplasm By similarity HAMAP MF_01035.

Sequence similarities

Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 2 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3433433-isopropylmalate dehydrogenase HAMAP MF_01035
PRO_1000135854

Regions

Nucleotide binding278 – 29013NAD By similarity

Sites

Metal binding2181Magnesium or manganese By similarity
Metal binding2421Magnesium or manganese By similarity
Metal binding2461Magnesium or manganese By similarity
Binding site941Substrate By similarity
Binding site1041Substrate By similarity
Binding site1281Substrate By similarity
Binding site2181Substrate By similarity
Site1351Important for catalysis By similarity
Site1851Important for catalysis By similarity

Sequences

Sequence LengthMass (Da)Tools
B3DTF8 [UniParc].

Last modified July 22, 2008. Version 1.
Checksum: 143417C91B926D30

FASTA34337,246
        10         20         30         40         50         60 
MAKTYKIAVI PGDGIGKEVT PWAQKALEKA AEGVADFEYE NFDLGAERYL RDGAILPEDE 

        70         80         90        100        110        120 
EERIKANDAI LLGAVGDPRI KAGILERGLL LKLRFDLDQY VNLRPSKLYK GVTSPLANPG 

       130        140        150        160        170        180 
DIDFVVVREG TEGLYCGAGG AVRRNTPQEV ATEVSINTAY GVERVVRYAF KLAMKRKKHV 

       190        200        210        220        230        240 
TLVHKKNVLV NAGDMWQRIV DKVGEEYPEV THDYQHIDAA TIFLVSDPSR FDVILTDNLF 

       250        260        270        280        290        300 
GDILTDEAGS VVGGVGYSAS GCINASDEFP SMFEPIHGSA PDIAGQNKAN PTAAILSAAM 

       310        320        330        340 
LLEHLGFDDA AKKIHTAVEA DIEELGSTVR STDQVGKDIL ARM

« Hide

References

[1]"Comparative genomic analysis of the gut bacterium Bifidobacterium longum reveals loci susceptible to deletion during pure culture growth."
Lee J.H., Karamychev V.N., Kozyavkin S.A., Mills D., Pavlov A.R., Pavlova N.V., Polouchine N.N., Richardson P.M., Shakhova V.V., Slesarev A.I., Weimer B., O'Sullivan D.J.
BMC Genomics 9:247-247(2008) [PubMed: 18505588] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DJO10A.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000605 Genomic DNA. Translation: ACD98427.1.
RefSeqYP_001954925.1. NC_010816.1.

3D structure databases

ProteinModelPortalB3DTF8.
SMRB3DTF8. Positions 5-343.
ModBaseSearch...

Protein-protein interaction databases

STRINGB3DTF8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID6362649.
GenomeReviewsGene locus BLD_0981 in contig CP000605_GR.
KEGGblj:BLD_0981.
PATRIC21114914. VBIBifLon134794_0995.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG518924.
OMAAATIFMV.
ProtClustDBPRK03437.

Family and domain databases

HAMAPMF_01035. LeuB_type2.
[Tree]
InterProIPR023698. 3-isopropylmalate_DH_LeuB.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
Gene3DG3DSA:3.40.718.10. IDH_IMDH. 1 hit.
KOK00052.
PANTHERPTHR11835. IDH_IMDH_dimeric. 1 hit.
PfamPF00180. Iso_dh. 1 hit.
[Graphical view]
PROSITEPS00470. IDH_IMDH. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLEU3_BIFLD
AccessionPrimary (citable) accession number: B3DTF8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 22, 2008
Last modified: January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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