Skip Header

Contribute Send feedback
Read comments (?) or add your own

B3DTE2 (RPOB_BIFLD) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 27. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-directed RNA polymerase subunit beta
Short name=RNAP subunit beta
EC=2.7.7.6
Alternative name(s):
RNA polymerase subunit beta
Transcriptase subunit beta
Gene names
Name:rpoB
Ordered Locus Names:BLD_0965
OrganismBifidobacterium longum (strain DJO10A) [Complete proteome] [HAMAP]
Taxonomic identifier205913 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length1187 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates By similarity. HAMAP MF_01321

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_01321

Subunit structure

The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.

Sequence similarities

Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
   Biological processTranscription
   Cellular componentDNA-directed RNA polymerase
   Molecular functionNucleotidyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular functionDNA binding

Inferred from electronic annotation. Source: InterPro

DNA-directed RNA polymerase activity

Inferred from electronic annotation. Source: UniProtKB-KW

ribonucleoside binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11871187DNA-directed RNA polymerase subunit beta HAMAP MF_01321
PRO_1000141661

Sequences

Sequence LengthMass (Da)Tools
B3DTE2 [UniParc].

Last modified July 22, 2008. Version 1.
Checksum: 42DCB12C1D7F31C8

FASTA1,187131,644
        10         20         30         40         50         60 
MATESTTNTT TIIARADQHD IDLHKASDRV NFGSIKEPID VPYLLGVQTD SFDWLIGNER 

        70         80         90        100        110        120 
WKARVEEDEK NGTNTVAHTS GLDEVFNEIS PIENFAQTMS LTFSDPYFEE PRHTVQECKE 

       130        140        150        160        170        180 
KDYTYSAPLY VNAEFENGDT GEIKSQTVFM GDFPLQTPHG TFIIGGTERV IVSQLVRSPG 

       190        200        210        220        230        240 
VYFDRQQDRT SDKEVFGAKI IPSRGAWLEF EIDKKDQPQV RVDRKRKQSA IVFLMAIGMT 

       250        260        270        280        290        300 
KSEIAQAFKD YPLVLDALEK ETLGTQDEAL VDLYRKIRPA DTPTPEAGKN LLDSFYFNTK 

       310        320        330        340        350        360 
RYDLARVGRY KINRKLGVEA DFNDRSLHQE DIIATIKYLV ALHDGAATFP GKRNGEDVDL 

       370        380        390        400        410        420 
RVDVDDIDHF GNRRIRQVGE LIQNQLRTGL SRMERVVRER MTTQDAEAIT PQSLINIRPV 

       430        440        450        460        470        480 
NATIKEFFGT SQLSQFMDQN NPLSGVTNKR RLSALGPGGL SRDRASMEVR DVHPSHFGRM 

       490        500        510        520        530        540 
CPIESPEGPN IGLIGSLATF GRVNPFGFIE TPYRKVVNGH VTDEVEYMTA DRDLDHVIAQ 

       550        560        570        580        590        600 
ANQELDENGN FVQKSALARV GEEEAVDVPV SSVDYMDVSP RQMVSLGASL IPFLEHDEGH 

       610        620        630        640        650        660 
RALMGTNMQR QAVPLIESER PLVGTGSEWR AANDSGDVIK SEKDGVVTYV SADLIRVMND 

       670        680        690        700        710        720 
DGTTSSYKLA KFQRSNQTTC YNQRPIVHDG ERVEAGSVMA DGPAIQNGDL ALGKNLLIAF 

       730        740        750        760        770        780 
MPWNGYNYED AVIISQRLVQ DDTLSSIHIE EYEIDARETK LGAEEITRDL PNVGEDAVAN 

       790        800        810        820        830        840 
LDERGIIRIG AEVEAGDILV GKVTPKGETE LTPEERLLRA IFGEKSREVR DTSLRVPHGE 

       850        860        870        880        890        900 
TGTVIGVKEI TREDAEEDGD ELPNGVNQMI RVYIAQHRKI TVGDKLSGRH GNKGCISRIL 

       910        920        930        940        950        960 
PEEDMPFLAD GTPVDIMLNP LGVPSRMNLG QVLELHLGWI AHSGWDISLD PNLEAEWKKL 

       970        980        990       1000       1010       1020 
IPSGAEKAEP NTPVATPVFD GVKPEVLKGL LSTTLPNRDG DRLVGPDGKA TLFDGRTGEP 

      1030       1040       1050       1060       1070       1080 
YTKPISVGYM YMLKLHHLVD DKIHARSTGP YSMITQQPLG GKAQFGGQRF GEMEVWALEA 

      1090       1100       1110       1120       1130       1140 
YGAAYTLHEM MTTKSDDVDG RVRVYGAIVK GDNLPPAGIP ESFKVLLKEM QSLSLNVEVL 

      1150       1160       1170       1180 
NAEGVAIDMK DEDDDPASSA DDLGFNIGAR PDAAAKEDQK AEEPEYQ

« Hide

References

[1]"Comparative genomic analysis of the gut bacterium Bifidobacterium longum reveals loci susceptible to deletion during pure culture growth."
Lee J.H., Karamychev V.N., Kozyavkin S.A., Mills D., Pavlov A.R., Pavlova N.V., Polouchine N.N., Richardson P.M., Shakhova V.V., Slesarev A.I., Weimer B., O'Sullivan D.J.
BMC Genomics 9:247-247(2008) [PubMed: 18505588] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DJO10A.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000605 Genomic DNA. Translation: ACD98411.1.
RefSeqYP_001954909.1. NC_010816.1.

3D structure databases

ProteinModelPortalB3DTE2.
ModBaseSearch...

Protein-protein interaction databases

STRINGB3DTE2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID6363194.
GenomeReviewsGene locus BLD_0965 in contig CP000605_GR.
KEGGblj:BLD_0965.
PATRIC21114880. VBIBifLon134794_0978.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG285547.
OMAQRDLTYA.
ProtClustDBPRK00405.

Family and domain databases

HAMAPMF_01321. RNApol_bact_RpoB.
[Tree]
InterProIPR010243. DNA-dir_RNA_pol_bsu_bac-type.
IPR019462. DNA-dir_RNA_pol_bsu_external_1.
IPR015712. DNA-dir_RNA_pol_su2.
IPR007120. DNA-dir_RNA_pol_su2_6.
IPR007121. RNA_pol_bsu_CS.
IPR007644. RNA_pol_bsu_protrusion.
IPR007642. RNA_pol_Rpb2_2.
IPR007645. RNA_pol_Rpb2_3.
IPR007641. RNA_pol_Rpb2_7.
IPR014724. RNA_pol_RPB2_OB-fold.
[Graphical view]
Gene3DG3DSA:2.30.150.10. G3DSA:2.30.150.10. 1 hit.
G3DSA:2.40.270.10. G3DSA:2.40.270.10. 2 hits.
G3DSA:2.40.50.150. Ribosomal_L2. 1 hit.
KOK03043.
PANTHERPTHR20856:SF3. PTHR20856:SF3. 1 hit.
PTHR20856. RNA_pol_I_sub2. 1 hit.
PfamPF04563. RNA_pol_Rpb2_1. 1 hit.
PF04561. RNA_pol_Rpb2_2. 1 hit.
PF04565. RNA_pol_Rpb2_3. 1 hit.
PF10385. RNA_pol_Rpb2_45. 1 hit.
PF00562. RNA_pol_Rpb2_6. 1 hit.
PF04560. RNA_pol_Rpb2_7. 1 hit.
[Graphical view]
TIGRFAMsTIGR02013. RpoB. 1 hit.
PROSITEPS01166. RNA_POL_BETA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRPOB_BIFLD
AccessionPrimary (citable) accession number: B3DTE2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 22, 2008
Last modified: January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents