Reviewed, UniProtKB/Swiss-Prot B3DT37 (PUR7_BIFLD)

Names and origin

Protein names

Recommended name:
    Phosphoribosylaminoimidazole-succinocarboxamide synthase
    EC=6.3.2.6
Alternative name(s):
    SAICAR synthetase

Gene names

Name:	purC
Ordered Locus Names:	BLD_0860

Organism

Bifidobacterium longum (strain DJO10A) [Complete proteome] [HAMAP]

Taxonomic identifier

205913 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	250 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate. HAMAP MF_00137
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2. HAMAP MF_00137
Sequence similarities	Belongs to the SAICAR synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylaminoimidazolesuccinocarboxamide synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 250	250	Phosphoribosylaminoimidazole-succinocarboxamide synthase HAMAP MF_00137		PRO_1000095965

Sequences

	Sequence		Length	Mass (Da)
	B3DT37-1 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 5874EA8DA5287E98	FASTA	250	28,220
	10 20 30 40 50 60 MEKLEKLYEG KAKQLYATDD PEVLWVEYKN TATAGDGEKK EDFTGKGRLN NLITTIIFDL 70 80 90 100 110 120 LKKRGIDSHL IKRVDDTGQL VRKVNMFPLE IVLRNVAAGH FCSRLGVEEG LPLKEPVLEY 130 140 150 160 170 180 FLKNDDLHDP FVNDDDLVAL GVCTREDLAE IAPLARKINE ALIEIFAKID VKLVDFKIEM 190 200 210 220 230 240 GRATDGTLLL ADEITPDSCR LWDQKDHSGK VEHLDKDLFR RGLGSIIPAY EEIEERLAEL 250 AKSEGIEVAE

References

[1]

"Comparative genomic analysis of the gut bacterium Bifidobacterium longum reveals loci susceptible to deletion during pure culture growth." Lee J.H., Karamychev V.N., Kozyavkin S.A., Mills D., Pavlov A.R., Pavlova N.V., Polouchine N.N., Richardson P.M., Shakhova V.V., Slesarev A.I., Weimer B., O'Sullivan D.J. BMC Genomics 9:247-247(2008) [PubMed: 18505588] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000605 Genomic DNA. Translation: ACD98306.1.
RefSeq	YP_001954804.1.
3D structure databases
SMR	B3DT37. Positions 2-238.
ModBase	Search...
Genome annotation databases
GeneID	6362788.
GenomeReviews	Gene locus BLD_0860 in contig CP000605_GR.
KEGG	blj:BLD_0860.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG306070.
OMA	TAFNAQK.
Family and domain databases
HAMAP	MF_00137. SAICAR_synth. [Tree]
InterPro	IPR013816. ATP_grasp_subdomain_2. IPR001636. SAICAR_synt. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.
PANTHER	PTHR11609. SAICAR_synt. 1 hit.
Pfam	PF01259. SAICAR_synt. 1 hit. [Graphical view]
TIGRFAMs	TIGR00081. purC. 1 hit.
PROSITE	PS01057. SAICAR_SYNTHETASE_1. False negative. PS01058. SAICAR_SYNTHETASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR7_BIFLD

Accession

Primary (citable) accession number: B3DT37

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 22, 2008
Last modified:	February 9, 2010
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)