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B3DSX1 (NADK_BIFLD) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NAD kinase

EC=2.7.1.23
Alternative name(s):
ATP-dependent NAD kinase
Gene names
Name:nadK
Ordered Locus Names:BLD_0794
OrganismBifidobacterium longum (strain DJO10A) [Complete proteome] [HAMAP]
Taxonomic identifier205913 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeBifidobacterialesBifidobacteriaceaeBifidobacterium

Protein attributes

Sequence length340 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP By similarity. HAMAP-Rule MF_00361

Catalytic activity

ATP + NAD+ = ADP + NADP+. HAMAP-Rule MF_00361

Cofactor

Divalent metal ions By similarity. HAMAP-Rule MF_00361

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00361.

Sequence similarities

Belongs to the NAD kinase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
NAD
NADP
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processNAD metabolic process

Inferred from electronic annotation. Source: InterPro

NADP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

NAD+ kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 340340NAD kinase HAMAP-Rule MF_00361
PRO_1000120831

Regions

Nucleotide binding66 – 672NAD By similarity
Nucleotide binding141 – 1422NAD By similarity
Nucleotide binding182 – 1876NAD By similarity

Sites

Active site661Proton acceptor By similarity
Binding site711NAD By similarity
Binding site1521NAD By similarity
Binding site1711NAD By similarity
Binding site2061NAD; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
B3DSX1 [UniParc].

Last modified July 22, 2008. Version 1.
Checksum: 80358D7A76FC86C0

FASTA34036,472
        10         20         30         40         50         60 
MAKRNAVVVT HTRLRQTGTV VAEAVSQLRV AGFEVAIIDN TEAPDFGVQP PCVSDDTEIV 

        70         80         90        100        110        120 
VVLGGDGTIL RAAELVHCTQ VPILGVNMGH VGFLAEFESF QIDEAIRRVS THDYSIDERM 

       130        140        150        160        170        180 
IAHVDVWLPG ATKPIEDWAL NDITLERADR GKMVELSIRV DDVEMNSFGA DGVIVSTPTG 

       190        200        210        220        230        240 
STAYAFSAGG PVMWPNVKAL QLIPLAAHAL FARPLIIGSG STFTIDILDD SMSEGWICCD 

       250        260        270        280        290        300 
GRRQRALPQG TRVMVRESRD TLRLARLSGV PFTNRLVSKF DLPVVGWREH ARNEASSQSL 

       310        320        330        340 
HHGHTFPAAA YAAGVAVAGD AGVAGTEPDK PGERDGKAGS 

« Hide

References

[1]"Comparative genomic analysis of the gut bacterium Bifidobacterium longum reveals loci susceptible to deletion during pure culture growth."
Lee J.H., Karamychev V.N., Kozyavkin S.A., Mills D., Pavlov A.R., Pavlova N.V., Polouchine N.N., Richardson P.M., Shakhova V.V., Slesarev A.I., Weimer B., O'Sullivan D.J.
BMC Genomics 9:247-247(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DJO10A.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000605 Genomic DNA. Translation: ACD98240.1.
RefSeqYP_001954738.1. NC_010816.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING205913.BLD_0794.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACD98240; ACD98240; BLD_0794.
GeneID6362908.
KEGGblj:BLD_0794.
PATRIC21114532. VBIBifLon134794_0806.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0061.
HOGENOMHOG000227223.
KOK00858.
OMAGVLWCDG.
OrthoDBEOG6PZXDR.

Enzyme and pathway databases

BioCycBLON205913:GJB4-822-MONOMER.

Family and domain databases

Gene3D2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPMF_00361. NAD_kinase.
InterProIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERPTHR20275. PTHR20275. 1 hit.
PfamPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMSSF111331. SSF111331. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNADK_BIFLD
AccessionPrimary (citable) accession number: B3DSX1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 22, 2008
Last modified: July 9, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families