B3DSM9 (B3DSM9_BIFLD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing] HAMAP MF_00344
EC=6.3.5.2 HAMAP MF_00344

Alternative name(s):
GMP synthetase HAMAP MF_00344
Glutamine amidotransferase HAMAP MF_00344

Gene names

Name:	guaA1 EMBL ACD98148.1
Synonyms:	guaA HAMAP MF_00344
Ordered Locus Names:	BLD_0702

Organism

Bifidobacterium longum (strain DJO10A) [Complete proteome] [HAMAP]

Taxonomic identifier

205913 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	520 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_00344
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00344
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00344
Subunit structure	Homodimer By similarity. HAMAP MF_00344
Sequence similarities	Contains 1 GMP-binding domain. HAMAP MF_00344 Contains 1 glutamine amidotransferase type-1 domain. HAMAP MF_00344

Ontologies

Keywords
Biological process	GMP biosynthesis HAMAP MF_00344 Purine biosynthesis
Domain	Glutamine amidotransferase HAMAP MF_00344
Ligand	ATP-binding HAMAP MF_00344 Nucleotide-binding
Molecular function	Ligase HAMAP MF_00344
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Domain	5 – 195	191	Glutamine amidotransferase type-1 By similarity HAMAP MF_00344
	Domain	227 – 395	169	GMP-binding By similarity HAMAP MF_00344
	Nucleotide binding	223 – 229	7	ATP By similarity HAMAP MF_00344
Sites
	Active site	82	1	Nucleophile By similarity HAMAP MF_00344
	Active site	169	1	By similarity HAMAP MF_00344
	Active site	171	1	By similarity HAMAP MF_00344

Sequences

	Sequence		Length	Mass (Da)
	B3DSM9 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: F9A6E3BA4860C312	FASTA	520	56,152
	10 20 30 40 50 60 MANGPVLVVD FGAQYAQLIA RRVREAGVYS ELVPHSMPVD EILAKDPKAI ILSGGPASVF 70 80 90 100 110 120 EPGAPTIDTK VFESGVPVLG ICYGFQVMAY ELGGKVDKAA LGEYGKTSAT IDDAAGILAD 130 140 150 160 170 180 SPAEQTTWMS HGVAVEQAPA GFEVLAHTEG APVAAMADES RKLYGVQWHP EVKHSPLGQK 190 200 210 220 230 240 LIENFLHRCA ALPNDWDASS IIEDQVKKIR EQVGDAEVIC GLSGGVDSAV AAALVHKAIG 250 260 270 280 290 300 DQLTCVFVDH GLLRKGEVEQ VKHDFVAATG IRLITVDAAD DFLDALAGVS EPERKRKIIG 310 320 330 340 350 360 EKFIRTFEKA QRQVLEEAGA RGKEVKFLVQ GTLYPDVVES GGGDGAANIK SHHNVGGLPE 370 380 390 400 410 420 DIKFQLIEPL RTLFKDEVRA IGTELGLPDE IVWRQPFPGP GLGIRIIGEI TKERLDLLRE 430 440 450 460 470 480 ADAIAREELS KAGLDRDIWQ CPVVLLADVH SVGVQGDERT YGSPIVLRPV SSEDAMTADW 490 500 510 520 SRVPYDVLAT ISTRITNECR QINRVVLDCT SKPPATIEWE

References

[1]

"Comparative genomic analysis of the gut bacterium Bifidobacterium longum reveals loci susceptible to deletion during pure culture growth." Lee J.H., Karamychev V.N., Kozyavkin S.A., Mills D., Pavlov A.R., Pavlova N.V., Polouchine N.N., Richardson P.M., Shakhova V.V., Slesarev A.I., Weimer B., O'Sullivan D.J. BMC Genomics 9:247-247(2008) [PubMed: 18505588] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000605 Genomic DNA. Translation: ACD98148.1.
RefSeq	YP_001954646.1. NC_010816.1.
3D structure databases
ProteinModelPortal	B3DSM9.
ModBase	Search...
Protein-protein interaction databases
STRING	B3DSM9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6363241.
GenomeReviews	Gene locus BLD_0702 in contig CP000605_GR.
KEGG	blj:BLD_0702.
PATRIC	21114342. VBIBifLon134794_0716.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG391775.
OMA	DGRTYEY.
ProtClustDB	PRK00074.
Family and domain databases
HAMAP	MF_00344. GMP_synthase. [Tree]
InterPro	IPR017926. GATASE_1. IPR001674. GMP_synth_C. IPR004739. GMP_synth_N. IPR022955. GMP_synthase. IPR022310. NAD/GMP_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01951.
Pfam	PF00117. GATase. 1 hit. PF00958. GMP_synt_C. 1 hit. PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00884. GuaA_Cterm. 1 hit. TIGR00888. GuaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B3DSM9_BIFLD

Accession

Primary (citable) accession number: B3DSM9

Entry history

Integrated into UniProtKB/TrEMBL:	July 22, 2008
Last sequence update:	July 22, 2008
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)