B3DSL3 (B3DSL3_BIFLD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
ATP-dependent Clp protease proteolytic subunit 2 HAMAP MF_00444
EC=3.4.21.92 HAMAP MF_00444

Alternative name(s):
Endopeptidase Clp 2 HAMAP MF_00444

Gene names

Name:	clpP2 HAMAP MF_00444 EMBL ACD98132.1
Ordered Locus Names:	BLD_0686

Organism

Bifidobacterium longum (strain DJO10A) [Complete proteome] [HAMAP]

Taxonomic identifier

205913 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	207 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins By similarity. HAMAP MF_00444
Catalytic activity	Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs). HAMAP MF_00444
Subcellular location	Cytoplasm By similarity HAMAP MF_00444.
Sequence similarities	Belongs to the peptidase S14 family. RuleBase RU003567 HAMAP MF_00444

Ontologies

Keywords
Cellular component	Cytoplasm HAMAP MF_00444
Ligand	ATP-binding HAMAP MF_00444 Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease HAMAP MF_00444
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	102	1	By similarity HAMAP MF_00444
	Active site	127	1	By similarity HAMAP MF_00444

Sequences

	Sequence		Length	Mass (Da)
	B3DSL3 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: B2056BCDBD66B3DB	FASTA	207	22,724
	10 20 30 40 50 60 MSNTFATLPV MAGDDVPAGP VDPIFNRLLK DRIIWMGEEV KDDMANRICA QLLMLAAEDP 70 80 90 100 110 120 KKDIWLYINS PGGSITAGMA IYDTMQLIEP DVATVGLGMC ASMGQFLLSS GTKGKRYLTS 130 140 150 160 170 180 HARVLMHQPS GGIGGTATDV RINAELIMDM KKTMSELTAE QTGHTLEEIY RDNEYDHWFT 190 200 AQEALDYGFV DKLVTTPDTI GNNQQGE

References

[1]

"Comparative genomic analysis of the gut bacterium Bifidobacterium longum reveals loci susceptible to deletion during pure culture growth." Lee J.H., Karamychev V.N., Kozyavkin S.A., Mills D., Pavlov A.R., Pavlova N.V., Polouchine N.N., Richardson P.M., Shakhova V.V., Slesarev A.I., Weimer B., O'Sullivan D.J. BMC Genomics 9:247-247(2008) [PubMed: 18505588] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000605 Genomic DNA. Translation: ACD98132.1.
RefSeq	YP_001954630.1. NC_010816.1.
3D structure databases
ProteinModelPortal	B3DSL3.
SMR	B3DSL3. Positions 23-196.
ModBase	Search...
Protein-protein interaction databases
STRING	B3DSL3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6364212.
GenomeReviews	Gene locus BLD_0686 in contig CP000605_GR.
KEGG	blj:BLD_0686.
PATRIC	21114308. VBIBifLon134794_0699.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG558421.
OMA	DGVANSL.
ProtClustDB	CLSK572836.
Family and domain databases
HAMAP	MF_00444. ClpP. [Tree]
InterPro	IPR023562. Pept_S14/S49. IPR001907. Pept_S14_ClpP. [Graphical view]
KO	K01358.
PANTHER	PTHR10381. Pept_S14_ClpP. 1 hit.
Pfam	PF00574. CLP_protease. 1 hit. [Graphical view]
PRINTS	PR00127. CLPPROTEASEP.
ProtoNet	Search...

Entry information

Entry name

B3DSL3_BIFLD

Accession

Primary (citable) accession number: B3DSL3

Entry history

Integrated into UniProtKB/TrEMBL:	July 22, 2008
Last sequence update:	July 22, 2008
Last modified:	December 14, 2011
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)