Reviewed, UniProtKB/Swiss-Prot B3DS65 (METE_BIFLD)

Names and origin

Protein names

Recommended name:
    5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
    EC=2.1.1.14
Alternative name(s):
    Methionine synthase, vitamin-B12 independent isozyme
    Cobalamin-independent methionine synthase

Gene names

Name:	metE
Ordered Locus Names:	BLD_0538

Organism

Bifidobacterium longum (strain DJO10A) [Complete proteome] [HAMAP]

Taxonomic identifier

205913 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	767 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity.
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 767	767	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000097815
Sites
	Metal binding	652	1	Zinc By similarity
	Metal binding	654	1	Zinc By similarity
	Metal binding	737	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3DS65-1 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 839AC629629F9D26	FASTA	767	85,359
	10 20 30 40 50 60 MSTLTSVSGF PRIGQNRELK KIIEGYWKGA NDLAAVKATA AELRAKHWKL QQAAGIDLIP 70 80 90 100 110 120 SNDFSYYDQM LDTAILLNVI PQRYARLSFD NQEDTLFAMA RGYQGDKGDV TALPMKKWFT 130 140 150 160 170 180 TNYHYLVPEV ESAAEIKLNS TKPFDEFNEA KALGIDTKPV FIGPYTFLKL ARTPEATELE 190 200 210 220 230 240 LDKGLVNAVA AVYVEVLAKF NELGAAWVQL DEPYLVLDKE PGDVELFKTL YTKILSAKGN 250 260 270 280 290 300 VKVLLNTYFG HIADVYETVN LLGFDGIGLD LNEGREENLE AVAKYGVASN TTIFAGVING 310 320 330 340 350 360 RNIWRNNYAT SLGLVDALKQ VTANVAVSTA SSLLHVPFST EGETGIPAED LKHFAFAVQK 370 380 390 400 410 420 LDELKEVAAL ADATEDEKKA SAALAANQAL FDGTRVAADP AVAERIGKLS DADYVRQPAR 430 440 450 460 470 480 EERQALQREA LGLPLLPTTT IGSFPQTKEI RAERAKLRKG EVTKEAYDEF IKAQIDAVIK 490 500 510 520 530 540 KQEEIGLDVL VHGEFERNDM VEYFGQNLNG FLFTKNAWVQ SYGTRCVKPP IVWGDVSRAN 550 560 570 580 590 600 PITVEWSAYA QSKTDHVMKG MLTGPVTILN WSWPREDITH EEQTKQLALA IRDEVLDLEA 610 620 630 640 650 660 AGIKVIQIDE AALREKLPLR KSDWHVKYLD WAVPAFRLVH SAVKPTTQIH THMCYSEFND 670 680 690 700 710 720 IIRDIDAMDA DVISFEASRG DLVVLDAIHD AHFETEAGPG VYDIHSPRIP SEKEIEDRIY 730 740 750 760 EILDKMDVKK VWINPDCGLK TRGNAETWPS LENLVAAAKA VRAKLDK

References

[1]

"Comparative genomic analysis of the gut bacterium Bifidobacterium longum reveals loci susceptible to deletion during pure culture growth." Lee J.H., Karamychev V.N., Kozyavkin S.A., Mills D., Pavlov A.R., Pavlova N.V., Polouchine N.N., Richardson P.M., Shakhova V.V., Slesarev A.I., Weimer B., O'Sullivan D.J. BMC Genomics 9:247-247(2008) [PubMed: 18505588] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000605 Genomic DNA. Translation: ACD97984.1.
RefSeq	YP_001954482.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6363352.
GenomeReviews	Gene locus BLD_0538 in contig CP000605_GR.
KEGG	blj:BLD_0538.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	TYLWLSK.
Family and domain databases
HAMAP	MF_00172. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR002629. Methionine_synth. IPR006276. MeTrfase_B12_ind. [Graphical view]
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_BIFLD

Accession

Primary (citable) accession number: B3DS65

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 22, 2008
Last modified:	November 3, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)