B3DS61 (PYRB_BIFLD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate carbamoyltransferase
EC=2.1.3.2

Alternative name(s):
Aspartate transcarbamylase
Short name=ATCase

Gene names

Name:	pyrB
Ordered Locus Names:	BLD_0534

Organism

Bifidobacterium longum (strain DJO10A) [Complete proteome] [HAMAP]

Taxonomic identifier

205913 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate. HAMAP MF_00001
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3. HAMAP MF_00001
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro aspartate carbamoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 320	320	Aspartate carbamoyltransferase HAMAP MF_00001		PRO_1000088739

Sequences

	Sequence		Length	Mass (Da)
	B3DS61 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: AEC328C7F9EFA0DC	FASTA	320	35,264
	10 20 30 40 50 60 MVGKSVVTLD GLSTNQILDL LHKAEYIDSH RKEIAHTCDG RVLATLFYEP STRTRLSFET 70 80 90 100 110 120 AMLRLGGKVI GFAGAQLASV TKGESIADTL KTVSNYVDVV AIRHPKEGAA LVASRAASVP 130 140 150 160 170 180 VINAGDGGHM HPTQTLADLA TLQSRFGRIT DLTVGLCGDL TFGRTVHSLI ETLCRFGNVR 190 200 210 220 230 240 FVLISPDELK TPQYVIDRIN ATDSCSYVEV RDLASVIGDL DVLYMTRVQK ERFFNEDDYL 250 260 270 280 290 300 RLRDTYILDE EKLQLAKPSM AVLHPLPRVN EIAVDVDDDP RAAYFEQVKN GMLVRMALES 310 320 TVVGDELPGY EPLNPKEVQA

References

[1]

"Comparative genomic analysis of the gut bacterium Bifidobacterium longum reveals loci susceptible to deletion during pure culture growth." Lee J.H., Karamychev V.N., Kozyavkin S.A., Mills D., Pavlov A.R., Pavlova N.V., Polouchine N.N., Richardson P.M., Shakhova V.V., Slesarev A.I., Weimer B., O'Sullivan D.J. BMC Genomics 9:247-247(2008) [PubMed: 18505588] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DJO10A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000605 Genomic DNA. Translation: ACD97980.1.
RefSeq	YP_001954478.1. NC_010816.1.
3D structure databases
ProteinModelPortal	B3DS61.
SMR	B3DS61. Positions 1-305.
ModBase	Search...
Protein-protein interaction databases
STRING	B3DS61.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6363348.
GenomeReviews	Gene locus BLD_0534 in contig CP000605_GR.
KEGG	blj:BLD_0534.
PATRIC	21113994. VBIBifLon134794_0546.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG579429.
OMA	ADRYENT.
ProtClustDB	PRK00856.
Family and domain databases
HAMAP	MF_00001. Asp_carb_tr. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P-bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn-bd. IPR002082. Asp_carbamoyltransf_euk. [Graphical view]
KO	K00609.
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00101. ATCASE.
SUPFAM	SSF53671. Asp/Orn_carbamoyltranf. 1 hit.
TIGRFAMs	TIGR00670. Asp_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRB_BIFLD

Accession

Primary (citable) accession number: B3DS61

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 22, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program