B3DS59 (B3DS59_BIFLD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Dihydroorotase EMBL ACD97978.1

Gene names

Name:	pyrC EMBL ACD97978.1
Ordered Locus Names:	BLD_0532

Organism

Bifidobacterium longum (strain DJO10A) [Complete proteome] [HAMAP]

Taxonomic identifier

205913 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	498 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate. SAAS SAAS002195
Cofactor	Binds 2 zinc ions per subunit By similarity. SAAS SAAS002195
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3. SAAS SAAS002195
Subunit structure	Homodimer By similarity. SAAS SAAS002195
Sequence similarities	Belongs to the DHOase family. RuleBase RU004949

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis SAAS SAAS002195
Ligand	Metal-binding SAAS SAAS002195 RuleBase RU004949 Zinc SAAS SAAS002195
Molecular function	Hydrolase SAAS SAAS002195 RuleBase RU004949
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B3DS59 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 350F1F100198F265	FASTA	498	53,256
	10 20 30 40 50 60 MSITLRNIKV WNTGEVIDLV VPGTAAAYFA DTSAVADGAD IDATGLTVAP GFEDPHVHFR 70 80 90 100 110 120 DPGQTYKESM VSGCRASASG GYTNVLIMPN TLPALDGQTV SGPEATGAKE VLDAGFDNVI 130 140 150 160 170 180 DFLQQYDTAH DVQLPVRYDL CVCASKDRAG HEASDVADWL KYVPGFEDDA KTPAMLTHPI 190 200 210 220 230 240 TAISDDGSAV TPEILDQVLE NVKASDLYLI EHCEHHDTGA VNEGPVSREL GVPGIPEDTE 250 260 270 280 290 300 LKIVARDIEA ARRTGVHVHF QHVSTAISFE AIRRAKAEGL PITCETAPHY LALSDEALLK 310 320 330 340 350 360 YGTLAKMNPP LRSEADRKAT IAAIADGTVD LLATDHAPHT LAEKELGFLE APNGIIGLEC 370 380 390 400 410 420 AYGVCHKVLV DGGFISDERL IELMSVGPAE LMGHVPTDVA ALVEDYAEPA PTGDDPDGVI 430 440 450 460 470 480 AGESDAQPQR EGVNRLLDLS RVDDADNVDL VVLNTAEEWT VDPEQFHSKA RNTPFGGWQV 490 TGRPLATIIG SQLMFSRL

References

[1]

"Comparative genomic analysis of the gut bacterium Bifidobacterium longum reveals loci susceptible to deletion during pure culture growth." Lee J.H., Karamychev V.N., Kozyavkin S.A., Mills D., Pavlov A.R., Pavlova N.V., Polouchine N.N., Richardson P.M., Shakhova V.V., Slesarev A.I., Weimer B., O'Sullivan D.J. BMC Genomics 9:247-247(2008) [PubMed: 18505588] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000605 Genomic DNA. Translation: ACD97978.1.
RefSeq	YP_001954476.1. NC_010816.1.
3D structure databases
ProteinModelPortal	B3DS59.
ModBase	Search...
Protein-protein interaction databases
STRING	B3DS59.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6363346.
GenomeReviews	Gene locus BLD_0532 in contig CP000605_GR.
KEGG	blj:BLD_0532.
PATRIC	21113990. VBIBifLon134794_0544.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG724623.
OMA	CDVHPVG.
ProtClustDB	CLSK572777.
Family and domain databases
HAMAP	MF_00220_B. PyrC_type2_B. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR004722. DHOase. IPR002195. Dihydroorotase_CS. IPR011059. Metal-dep_hydrolase_composite. [Graphical view]
KO	K01465.
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
SUPFAM	SSF51338. Metalo_hydrolase. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. 1 hit. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B3DS59_BIFLD

Accession

Primary (citable) accession number: B3DS59

Entry history

Integrated into UniProtKB/TrEMBL:	July 22, 2008
Last sequence update:	July 22, 2008
Last modified:	December 14, 2011
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)