Reviewed, UniProtKB/Swiss-Prot B3DRY6 (PUR9_BIFLD)

Names and origin

Protein names

Recommended name:
    Bifunctional purine biosynthesis protein purH
Including the following 2 domains:
    1- Recommended name:
            Phosphoribosylaminoimidazolecarboxamide formyltransferase
              EC=2.1.2.3
        Alternative name(s):
            AICAR transformylase
    2- Recommended name:
            IMP cyclohydrolase
              EC=3.5.4.10
        Alternative name(s):
            Inosinicase
            IMP synthetase
            ATIC

Gene names

Name:	purH
Ordered Locus Names:	BLD_0459

Organism

Bifidobacterium longum (strain DJO10A) [Complete proteome] [HAMAP]

Taxonomic identifier

205913 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	545 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP MF_00139 IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP MF_00139
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1. HAMAP MF_00139 Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.
Domain	The IMP cyclohydrolase activity resides in the N-terminal region By similarity. HAMAP MF_00139
Sequence similarities	Belongs to the purH family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Hydrolase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	IMP cyclohydrolase activity Inferred from electronic annotation. Source: HAMAP phosphoribosylaminoimidazolecarboxamide formyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 545	545	Bifunctional purine biosynthesis protein purH HAMAP MF_00139		PRO_1000096041

Sequences

	Sequence		Length	Mass (Da)
	B3DRY6-1 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: AC98E086445D7312	FASTA	545	58,402
	10 20 30 40 50 60 MTNTNRPIRR ALVSVFHKEG IEVLAEAFVK AGTEVVSTGS TAKKLAELGV KVTEVSDVTG 70 80 90 100 110 120 FPECLDGRVK TLHPYIHAGI LADMTNPEHA KQLEEFGIKP FDLVVVNLYP FADTVRSGAN 130 140 150 160 170 180 EADTIEKIDI GGPSMVRGAA KNHATVAIVT DPADYALVAS RVADGTGFSL DERKWLAAKA 190 200 210 220 230 240 FAHTAAYDAT INEWTAKHWP KPASLDAVEV DKDDQGTEVD SAKFPAQFTR TWDRAHTLRY 250 260 270 280 290 300 GENSHQQAAL YIDPLNQTGF AHAEQLGGKP MSYNNYVDAD AAWRTVWDMA PAIAVAVVKH 310 320 330 340 350 360 NNPCGLAIGA TAAEAHKKAH ACDPMSAYGG VIACNSKVTL EMAESVRPIF TEVIVAPDYE 370 380 390 400 410 420 PAALELLQTK KKNLRILKVA EPPKGHEAIR QIDGGLLVQD TDLINAVGDD PDAWKLVAGE 430 440 450 460 470 480 AADADTLKDL VFAWRAIRCV KSNAILLAHD QATVGIGMGQ VNRVDSCHLA VERANTLADG 490 500 510 520 530 540 ADRATGAVAA SDAFFPFADG AQVLIDAGVK AIVQPGGSIR DEEVIEAAKK AGVTMYLTGT RHFFH

References

[1]

"Comparative genomic analysis of the gut bacterium Bifidobacterium longum reveals loci susceptible to deletion during pure culture growth." Lee J.H., Karamychev V.N., Kozyavkin S.A., Mills D., Pavlov A.R., Pavlova N.V., Polouchine N.N., Richardson P.M., Shakhova V.V., Slesarev A.I., Weimer B., O'Sullivan D.J. BMC Genomics 9:247-247(2008) [PubMed: 18505588] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000605 Genomic DNA. Translation: ACD97905.1.
RefSeq	YP_001954403.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6363931.
GenomeReviews	Gene locus BLD_0459 in contig CP000605_GR.
KEGG	blj:BLD_0459.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG498048.
OMA	VVKHVKS.
Family and domain databases
HAMAP	MF_00139. PurH. [Tree]
InterPro	IPR002695. AICARFT_IMPCHas. IPR013982. AICARFT_IMPCHase_bienz. IPR011607. MGS. [Graphical view]
PANTHER	PTHR11692. AICARFT_IMPCHas. 1 hit.
Pfam	PF01808. AICARFT_IMPCHas. 1 hit. PF02142. MGS. 1 hit. [Graphical view]
PIRSF	PIRSF000414. AICARFT_IMPCHas. 1 hit.
SMART	SM00798. AICARFT_IMPCHas. 1 hit. SM00851. MGS. 1 hit. [Graphical view]
TIGRFAMs	TIGR00355. purH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR9_BIFLD

Accession

Primary (citable) accession number: B3DRY6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 22, 2008
Last modified:	February 9, 2010
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)