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B3DRX8

- RUVC_BIFLD

UniProt

B3DRX8 - RUVC_BIFLD

Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Bifidobacterium longum (strain DJO10A)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 41 (01 Oct 2014)
      Sequence version 1 (22 Jul 2008)
      Previous versions | rss
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    Functioni

    Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.UniRule annotation

    Catalytic activityi

    Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

    Cofactori

    Binds 1 magnesium ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi7 – 71MagnesiumUniRule annotation
    Metal bindingi68 – 681MagnesiumUniRule annotation
    Metal bindingi141 – 1411MagnesiumUniRule annotation
    Metal bindingi144 – 1441MagnesiumUniRule annotation

    GO - Molecular functioni

    1. crossover junction endodeoxyribonuclease activity Source: UniProtKB-HAMAP
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. nucleic acid binding Source: InterPro

    GO - Biological processi

    1. DNA recombination Source: UniProtKB-HAMAP
    2. DNA repair Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase, Nuclease

    Keywords - Biological processi

    DNA damage, DNA recombination, DNA repair

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciBLON205913:GJB4-469-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Crossover junction endodeoxyribonuclease RuvCUniRule annotation (EC:3.1.22.4UniRule annotation)
    Alternative name(s):
    Holliday junction nuclease RuvCUniRule annotation
    Holliday junction resolvase RuvCUniRule annotation
    Gene namesi
    Name:ruvCUniRule annotation
    Ordered Locus Names:BLD_0451
    OrganismiBifidobacterium longum (strain DJO10A)
    Taxonomic identifieri205913 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeBifidobacterialesBifidobacteriaceaeBifidobacterium
    ProteomesiUP000002419: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 194194Crossover junction endodeoxyribonuclease RuvCPRO_1000090502Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi205913.BLD_0451.

    Structurei

    3D structure databases

    ProteinModelPortaliB3DRX8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RuvC family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0817.
    HOGENOMiHOG000012182.
    KOiK01159.
    OMAiRQVQEMV.
    OrthoDBiEOG6RG044.

    Family and domain databases

    Gene3Di3.30.420.10. 1 hit.
    HAMAPiMF_00034. RuvC.
    InterProiIPR012337. RNaseH-like_dom.
    IPR020563. X-over_junc_endoDNase_Mg_BS.
    IPR002176. X-over_junc_endoDNase_RuvC.
    [Graphical view]
    PfamiPF02075. RuvC. 1 hit.
    [Graphical view]
    PRINTSiPR00696. RSOLVASERUVC.
    SUPFAMiSSF53098. SSF53098. 1 hit.
    TIGRFAMsiTIGR00228. ruvC. 1 hit.
    PROSITEiPS01321. RUVC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B3DRX8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIILGVDPGL TRCGVGVIEA GEHRRLSFIH VDVVRSSPDI TQDLRLLAIY    50
    NGLSEKMDRF APDAVSIERV FAQSNRNTVL GTAQAAGLAM LAAAQRNIPV 100
    ALHTPTEAKL AITGNGQAQK IQVERMVTRI LNLTKMPTPA DAADALALAI 150
    CHALRPAGAL QGGEREQHLT AAQRQWAEAA QNSTRRRINH DRGM 194
    Length:194
    Mass (Da):20,939
    Last modified:July 22, 2008 - v1
    Checksum:i806A0978709D968E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000605 Genomic DNA. Translation: ACD97897.1.
    RefSeqiYP_001954395.1. NC_010816.1.

    Genome annotation databases

    EnsemblBacteriaiACD97897; ACD97897; BLD_0451.
    GeneIDi6364204.
    KEGGiblj:BLD_0451.
    PATRICi21113836. VBIBifLon134794_0467.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000605 Genomic DNA. Translation: ACD97897.1 .
    RefSeqi YP_001954395.1. NC_010816.1.

    3D structure databases

    ProteinModelPortali B3DRX8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 205913.BLD_0451.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACD97897 ; ACD97897 ; BLD_0451 .
    GeneIDi 6364204.
    KEGGi blj:BLD_0451.
    PATRICi 21113836. VBIBifLon134794_0467.

    Phylogenomic databases

    eggNOGi COG0817.
    HOGENOMi HOG000012182.
    KOi K01159.
    OMAi RQVQEMV.
    OrthoDBi EOG6RG044.

    Enzyme and pathway databases

    BioCyci BLON205913:GJB4-469-MONOMER.

    Family and domain databases

    Gene3Di 3.30.420.10. 1 hit.
    HAMAPi MF_00034. RuvC.
    InterProi IPR012337. RNaseH-like_dom.
    IPR020563. X-over_junc_endoDNase_Mg_BS.
    IPR002176. X-over_junc_endoDNase_RuvC.
    [Graphical view ]
    Pfami PF02075. RuvC. 1 hit.
    [Graphical view ]
    PRINTSi PR00696. RSOLVASERUVC.
    SUPFAMi SSF53098. SSF53098. 1 hit.
    TIGRFAMsi TIGR00228. ruvC. 1 hit.
    PROSITEi PS01321. RUVC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Comparative genomic analysis of the gut bacterium Bifidobacterium longum reveals loci susceptible to deletion during pure culture growth."
      Lee J.H., Karamychev V.N., Kozyavkin S.A., Mills D., Pavlov A.R., Pavlova N.V., Polouchine N.N., Richardson P.M., Shakhova V.V., Slesarev A.I., Weimer B., O'Sullivan D.J.
      BMC Genomics 9:247-247(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DJO10A.

    Entry informationi

    Entry nameiRUVC_BIFLD
    AccessioniPrimary (citable) accession number: B3DRX8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: July 22, 2008
    Last modified: October 1, 2014
    This is version 41 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3