B3DRM8 (B3DRM8_BIFLD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase HAMAP MF_00012
Short name=DAD HAMAP MF_00012
EC=4.2.1.9 HAMAP MF_00012

Gene names

Name:	ilvD HAMAP MF_00012 EMBL ACD99410.1
Ordered Locus Names:	BLD_1965

Organism

Bifidobacterium longum (strain DJO10A) [Complete proteome] [HAMAP]

Taxonomic identifier

205913 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	589 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster By similarity. HAMAP MF_00012
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family. HAMAP MF_00012

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis HAMAP MF_00012
Ligand	4Fe-4S HAMAP MF_00012 Iron Iron-sulfur Metal-binding
Molecular function	Lyase HAMAP MF_00012
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	92	1	Iron-sulfur (4Fe-4S) By similarity HAMAP MF_00012
	Metal binding	166	1	Iron-sulfur (4Fe-4S) By similarity HAMAP MF_00012

Sequences

	Sequence		Length	Mass (Da)
	B3DRM8 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 639D1FD30FBECC46	FASTA	589	63,216
	10 20 30 40 50 60 MGKKPIIAIA NSFDEFLPGH VHLNKVGRIV SEAIKEAGGI PREFNTMAVD DGIAMGHTGM 70 80 90 100 110 120 LYSLPSRDII ADTVEYQCNA HCADALICIP NCDKVVPGML MAALRLNIPT VFVSGGPMEA 130 140 150 160 170 180 GTTVLADGTV KSTDLIDVMY ATADDSVSDE ELLNYEKTVC PTCGSCAGMF TANSMNCLTE 190 200 210 220 230 240 AIGLALPGNG TILASHSYRK DLFERAAKQV VKIAHQYYDD SDDSVLPRSI ATKEAFENAM 250 260 270 280 290 300 TMDVAMGGST NTVLHILAMA QSADVDFTLD DIERISHTVP CICKASPSGK WEISDVHRAG 310 320 330 340 350 360 GITGILGELD RAGKLHTNVH SIDYPTLEAK LADWDIMRPT CTEEAQQMYK AAPGHIISPE 370 380 390 400 410 420 PWTHTTLFDS LDRDRANGAI HDINHPEIHE GGLAVLRGNL APDGCVVKTA GVPPEIWKFR 430 440 450 460 470 480 GPALVVDSQE QAIEVILNDT LKPGMALVIR YEGPKGGPGM QEMLYPTSFV KGKGIGKQVA 490 500 510 520 530 540 MLTDGRYSGG SSGLAIGHMA PEAANKGPVA LIKNGDIIDI DIEARSVNVE LTDEQLDERR 550 560 570 580 RELEAGDGYV AHRNRHVSQA LKAYAAFARS ADKGATRDPE LINKLSGLD

References

[1]

"Comparative genomic analysis of the gut bacterium Bifidobacterium longum reveals loci susceptible to deletion during pure culture growth." Lee J.H., Karamychev V.N., Kozyavkin S.A., Mills D., Pavlov A.R., Pavlova N.V., Polouchine N.N., Richardson P.M., Shakhova V.V., Slesarev A.I., Weimer B., O'Sullivan D.J. BMC Genomics 9:247-247(2008) [PubMed: 18505588] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000605 Genomic DNA. Translation: ACD99410.1.
RefSeq	YP_001955908.1. NC_010816.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B3DRM8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6363714.
GenomeReviews	Gene locus BLD_1965 in contig CP000605_GR.
KEGG	blj:BLD_1965.
PATRIC	21116976. VBIBifLon134794_1984.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG671001.
OMA	MALVIRY.
ProtClustDB	PRK12448.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B3DRM8_BIFLD

Accession

Primary (citable) accession number: B3DRM8

Entry history

Integrated into UniProtKB/TrEMBL:	July 22, 2008
Last sequence update:	July 22, 2008
Last modified:	December 14, 2011
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)