B3DQN2 (MURC_BIFLD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramate--L-alanine ligase
EC=6.3.2.8

Alternative name(s):
UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	BLD_0182

Organism

Bifidobacterium longum (strain DJO10A) [Complete proteome] [HAMAP]

Taxonomic identifier

205913 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	512 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00046
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm By similarity HAMAP MF_00046.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 512	512	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_1000091079
Regions
	Nucleotide binding	132 – 138	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B3DQN2 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: A00E9213CDE3C549	FASTA	512	53,384
	10 20 30 40 50 60 MSQDQPIVLD PTYASFDAAA RPADLGATHF IGIGGAGMSV LAEMLHAEGV AVDGSDRAHS 70 80 90 100 110 120 AKTDRLETLG ITVEFGQRAE NVAQAETVVY SSAIKPDNPE IVAAHAAGKR IVHRSDILAL 130 140 150 160 170 180 LMNGKRAVTV AGAHGKTTTS SMLSHILVNA GADPSYAIGG FIQGPDGTTL DGGHAGKGDI 190 200 210 220 230 240 LVAEADESDG SFAKYHPTIA IITNCEADHL DHYGDEAHYR AAFVAHAGRA TGHVIISIDD 250 260 270 280 290 300 PDGLAVLEAL PADVKSHTVA YGTTARESLP DLGGAAYVWI ASESETAGSG VEQLTLHLPA 310 320 330 340 350 360 AVTAGEPVSQ SVALKVPGVH NARNAAAAIS AAVLLGVSPA DAANAAGTFL GAARRFQVRG 370 380 390 400 410 420 TVKQVTVVDD YAHHPTEIAA LLDAARRRYP DSTIRVIFQP HLFSRTKFFA HQFAKSLAKA 430 440 450 460 470 480 DDVIITGIFP AREKQADFPD ISPSTIVDAA AGLKDASAGT WIQPVEDMCL AAKMMAMRAH 490 500 510 HGDVIFTVGA GDITDMDQVL LTALEAHRES CE

References

[1]

"Comparative genomic analysis of the gut bacterium Bifidobacterium longum reveals loci susceptible to deletion during pure culture growth." Lee J.H., Karamychev V.N., Kozyavkin S.A., Mills D., Pavlov A.R., Pavlova N.V., Polouchine N.N., Richardson P.M., Shakhova V.V., Slesarev A.I., Weimer B., O'Sullivan D.J. BMC Genomics 9:247-247(2008) [PubMed: 18505588] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DJO10A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000605 Genomic DNA. Translation: ACD97628.1.
RefSeq	YP_001954126.1. NC_010816.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B3DQN2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6362983.
GenomeReviews	Gene locus BLD_0182 in contig CP000605_GR.
KEGG	blj:BLD_0182.
PATRIC	21113285. VBIBifLon134794_0201.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG476594.
OMA	YIDDYAH.
ProtClustDB	CLSK573014.
Family and domain databases
HAMAP	MF_00046. MurC. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01924.
PANTHER	PTHR23135:SF5. PTHR23135:SF5. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01082. MurC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_BIFLD

Accession

Primary (citable) accession number: B3DQN2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 22, 2008
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program