Reviewed, UniProtKB/Swiss-Prot B3DQM9 (MURD_BIFLD)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoylalanine--D-glutamate ligase
    EC=6.3.2.9
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
    D-glutamic acid-adding enzyme

Gene names

Name:	murD
Ordered Locus Names:	BLD_0179

Organism

Bifidobacterium longum (strain DJO10A) [Complete proteome] [HAMAP]

Taxonomic identifier

205913 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	481 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 481	481	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_1000130830
Regions
	Nucleotide binding	108 – 114	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B3DQM9-1 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 57DBF3DF9EAA27B8	FASTA	481	51,056
	10 20 30 40 50 60 MQVADKTVVI AGLGVSGTSL AEVLRERGTH VIGVDERKPE ADLHSFDDVD WDHVDYVMSS 70 80 90 100 110 120 PVFNPRTPFV LEAQRRGIPV MSEVEFAWQL RVNNERTGTP APWIGITGTN GKTSTTEMTS 130 140 150 160 170 180 EMLTACGLDA PTAGNIASGD MSMSLSRCAT NPQHDVLCVE LSSFQLHFTD SLALDCAAIT 190 200 210 220 230 240 NIADDHLDWH GGRENYAADK SKVFHNAKRA IVYNAQDAKV SELAAEAQTA EGCRKVGFTL 250 260 270 280 290 300 EAPQAGQIGI EDGWIVDRSG VAGGAVGESV RLAAITDFTH LAEPDGSLYP HLVADALTAL 310 320 330 340 350 360 ALVLGLGADR DTALKALTSF KPGGHRIETV AEAAVEGGSV RFVDDSKATN GHAARASLSS 370 380 390 400 410 420 FPAKSVIWIA GGLAKGSRFE DLVKDQAHTI KAAVIIGKDQ QPMIEAFASQ APDIPVTIID 430 440 450 460 470 480 PEDNDTVMDR AVEACGTYTA AGDIVLMAPA CASMDQFKSY ADRGNRFAAA AKTWSEVHGL H

References

[1]

"Comparative genomic analysis of the gut bacterium Bifidobacterium longum reveals loci susceptible to deletion during pure culture growth." Lee J.H., Karamychev V.N., Kozyavkin S.A., Mills D., Pavlov A.R., Pavlova N.V., Polouchine N.N., Richardson P.M., Shakhova V.V., Slesarev A.I., Weimer B., O'Sullivan D.J. BMC Genomics 9:247-247(2008) [PubMed: 18505588] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000605 Genomic DNA. Translation: ACD97625.1.
RefSeq	YP_001954123.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6362980.
GenomeReviews	Gene locus BLD_0179 in contig CP000605_GR.
KEGG	blj:BLD_0179.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	SEDHLDR.
Family and domain databases
HAMAP	MF_00639. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01087. murD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_BIFLD

Accession

Primary (citable) accession number: B3DQM9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 22, 2008
Last modified:	November 3, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)