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Reviewed, UniProtKB/Swiss-Prot B3DQ87 (LSPA_BIFLD)

Last modified November 3, 2009. Version 12. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Lipoprotein signal peptidase
    EC=3.4.23.36
Alternative name(s):
    Prolipoprotein signal peptidase
    Signal peptidase II
      Short name=SPase II
Gene names
Name: lspA
Ordered Locus Names: BLD_0140
OrganismBifidobacterium longum (strain DJO10A) [Complete proteome] [HAMAP]
Taxonomic identifier205913 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

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Protein attributes

Sequence length182 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

This protein specifically catalyzes the removal of signal peptides from prolipoproteins By similarity.

Catalytic activity

Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains. HAMAP MF_00161

Pathway

Protein modification; lipoprotein biosynthesis (signal peptide cleavage). HAMAP MF_00161

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity.

Sequence similarities

Belongs to the peptidase A8 family.

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Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
   Molecular functionAspartyl protease
Hydrolase
Protease
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processproteolysis

Inferred from electronic annotation. Source: InterPro

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

plasma membrane

Inferred from electronic annotation. Source: HAMAP

   Molecular functionaspartic-type endopeptidase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 182182Lipoprotein signal peptidase HAMAP MF_00161
PRO_1000097230

Regions

Transmembrane12 – 3221 Potential
Transmembrane68 – 8821 Potential
Transmembrane91 – 11121 Potential
Transmembrane135 – 15521 Potential

Sites

Active site1121 By similarity
Active site1401 By similarity

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Sequences

Sequence LengthMass (Da)Tools
B3DQ87-1 [UniParc].

Last modified July 22, 2008. Version 1.
Checksum: A2DFB8E563C59843

FASTA18219,158
        10         20         30         40         50         60 
MTNQQGRLRT RVAVFACVAA AALIVDQLTK AWAMAALSNG QTIRVIPGLL SFTLVRNPGA 

        70         80         90        100        110        120 
SLGMGSGATW VISLLAVVAC VALAVAGVRT VSMKWSVAIS FAFAGALGNL IDRVMYADGF 

       130        140        150        160        170        180 
LDGKVVDFLN YGWSVGNVAD IYLVVAGVVL VILILMGEPF SHKDLIEQSD ESLQSEPEAD 


AK

« Hide

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References

[1]"Comparative genomic analysis of the gut bacterium Bifidobacterium longum reveals loci susceptible to deletion during pure culture growth."
Lee J.H., Karamychev V.N., Kozyavkin S.A., Mills D., Pavlov A.R., Pavlova N.V., Polouchine N.N., Richardson P.M., Shakhova V.V., Slesarev A.I., Weimer B., O'Sullivan D.J.
BMC Genomics 9:247-247(2008) [PubMed: 18505588] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases

CP000605 Genomic DNA. Translation: ACD97586.1.
RefSeqYP_001954084.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID6363091.
GenomeReviewsGene locus BLD_0140 in contig CP000605_GR.
KEGGblj:BLD_0140.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMAVVAKLEH.

Family and domain databases

HAMAPMF_00161.
[Tree]
InterProIPR001872. Peptidase_A8.
[Graphical view]
PfamPF01252. Peptidase_A8. 1 hit.
[Graphical view]
PRINTSPR00781. LIPOSIGPTASE.
TIGRFAMsTIGR00077. lspA. 1 hit.
PROSITEPS00855. SPASE_II. False negative.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameLSPA_BIFLD
AccessionPrimary (citable) accession number: B3DQ87
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 22, 2008
Last modified: November 3, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents