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Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Bifidobacterium longum (strain DJO10A)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Hydroxyethylthiazole kinase (thiM)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei70Substrate; via amide nitrogenUniRule annotation1
Binding sitei145ATPUniRule annotation1
Binding sitei217ATPUniRule annotation1
Binding sitei244Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
Short name:
TH kinaseUniRule annotation
Short name:
Thz kinaseUniRule annotation
Gene namesi
Name:thiMUniRule annotation
Ordered Locus Names:BLD_0133
OrganismiBifidobacterium longum (strain DJO10A)
Taxonomic identifieri205913 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaBifidobacterialesBifidobacteriaceaeBifidobacterium
Proteomesi
  • UP000002419 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003838241 – 314Hydroxyethylthiazole kinaseAdd BLAST314

Structurei

3D structure databases

ProteinModelPortaliB3DQ80.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000114352.
KOiK00878.
OMAiSPVMAHA.

Family and domain databases

CDDicd01170. THZ_kinase. 1 hit.
Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase. 1 hit.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.

Sequencei

Sequence statusi: Complete.

B3DQ80-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNSASSFTG VSSGYTAGTP VPADSPIRDN IADAVRRVRE TTPLAQSFTT
60 70 80 90 100
FVTINLVANA QLAAGGTAAM SFLPDDVIET AKIAGANYIN VGTLLPFYKD
110 120 130 140 150
ALPEIAQRLN YLDKPWVLDP VAAGIGRTRT AILQAFKAAP PTMIRANASE
160 170 180 190 200
VIALANMWGL NTETVGDASE HRPAGVESVD DVESATGAAV ALAQYLTEQH
210 220 230 240 250
AKHSSHDAST RCAVAVSGIA DLVTDGETVY RLPGGSAMMT KITGAGCSLG
260 270 280 290 300
GVAATYLAVS DPLTAALSAS LLYNRAGEVA DTTSHGPGSF QVAFLDALWN
310
VTAEQVAESE ILVQ
Length:314
Mass (Da):32,472
Last modified:July 22, 2008 - v1
Checksum:i4B11EF93566B3AA9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000605 Genomic DNA. Translation: ACD97579.1.
RefSeqiWP_012471774.1. NC_010816.1.

Genome annotation databases

EnsemblBacteriaiACD97579; ACD97579; BLD_0133.
KEGGiblj:BLD_0133.
PATRICi21113171. VBIBifLon134794_0150.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000605 Genomic DNA. Translation: ACD97579.1.
RefSeqiWP_012471774.1. NC_010816.1.

3D structure databases

ProteinModelPortaliB3DQ80.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACD97579; ACD97579; BLD_0133.
KEGGiblj:BLD_0133.
PATRICi21113171. VBIBifLon134794_0150.

Phylogenomic databases

HOGENOMiHOG000114352.
KOiK00878.
OMAiSPVMAHA.

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139.

Family and domain databases

CDDicd01170. THZ_kinase. 1 hit.
Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase. 1 hit.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTHIM_BIFLD
AccessioniPrimary (citable) accession number: B3DQ80
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 22, 2008
Last modified: November 2, 2016
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.