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Reviewed, UniProtKB/Swiss-Prot B3DQ31 (CARA_BIFLD)

Last modified February 9, 2010. Version 16. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Carbamoyl-phosphate synthase small chain
    EC=6.3.5.5
Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chain
Gene names
Name: carA
Ordered Locus Names: BLD_0084
OrganismBifidobacterium longum (strain DJO10A) [Complete proteome] [HAMAP]
Taxonomic identifier205913 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

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Protein attributes

Sequence length407 AA.
Sequence statusComplete.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1. HAMAP MF_01209

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3. HAMAP MF_01209

Subunit structure

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity. HAMAP MF_01209

Sequence similarities

Belongs to the carA family.

Contains 1 glutamine amidotransferase type-1 domain.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 407407Carbamoyl-phosphate synthase small chain HAMAP MF_01209
PRO_1000138851

Regions

Domain207 – 405199Glutamine amidotransferase type-1
Region1 – 203203CPSase HAMAP MF_01209

Sites

Active site2831Nucleophile By similarity
Active site3781 By similarity
Active site3801 By similarity

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Sequences

Sequence LengthMass (Da)Tools
B3DQ31-1 [UniParc].

Last modified July 22, 2008. Version 1.
Checksum: B9F825136ECD2B68

FASTA40744,194
        10         20         30         40         50         60 
MSQNESGTIA IPMYDKDDAV LVLEDGQVYV GEPYGALGET TGEIVFATGM TGYQETLTDP 

        70         80         90        100        110        120 
SYDRQIVVQT FPHIGDTGVN SEDPESSRIW VAGYIVRDPS PNVSNWRAEG SLDDDLAKNG 

       130        140        150        160        170        180 
IVGLSHIDTR KLVRHLRSAG VMRAGIFSGD ALTDQATGAL KTIEQLLEDV KNTPQMQGLS 

       190        200        210        220        230        240 
LYDEVSTKGT YTIEPCGEYE GKEPLYTVAA VDLGIKGMTP HRMAERGCRV HVVPSTITFA 

       250        260        270        280        290        300 
EIENLNPDGV FFSNGPGDPE QAGPEIELLR QVLDAGYPFF GICFGNQLLG RALGFGTYKL 

       310        320        330        340        350        360 
KFGHRGINQP VKDLTTGKVE VTAHNHGFAV DAPIGKQVDA PFENGKYGKV FVSHIDLNDD 

       370        380        390        400 
VVEGLQCVDI PAFSVQYHPE AAAGPHDAAY LFDRFCELMK NNSKEGK

« Hide

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References

[1]"Comparative genomic analysis of the gut bacterium Bifidobacterium longum reveals loci susceptible to deletion during pure culture growth."
Lee J.H., Karamychev V.N., Kozyavkin S.A., Mills D., Pavlov A.R., Pavlova N.V., Polouchine N.N., Richardson P.M., Shakhova V.V., Slesarev A.I., Weimer B., O'Sullivan D.J.
BMC Genomics 9:247-247(2008) [PubMed: 18505588] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000605 Genomic DNA. Translation: ACD97530.1.
RefSeqYP_001954028.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID6363634.
GenomeReviewsGene locus BLD_0084 in contig CP000605_GR.
KEGGblj:BLD_0084.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG286341.
OMAFTYPELG.

Family and domain databases

HAMAPMF_01209_B. CPSase_S_chain_B.
[Tree]
InterProIPR006220. Anth_synthII.
IPR001317. CarbamoylP_synth_GATase_dom.
IPR006274. CarbamoylP_synth_ssu.
IPR002474. CarbamoylP_synth_ssu_N.
IPR011702. GATASE.
IPR017926. GATASE_1.
IPR000991. GATase_class1_C.
[Graphical view]
PANTHERPTHR11405:SF4. CarA_synth_small. 1 hit.
PfamPF00988. CPSase_sm_chain. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
PRINTSPR00097. ANTSNTHASEII.
PR00099. CPSGATASE.
PR00096. GATASE.
TIGRFAMsTIGR01368. CPSaseIIsmall. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameCARA_BIFLD
AccessionPrimary (citable) accession number: B3DQ31
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 22, 2008
Last modified: February 9, 2010
This is version 16 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents