Reviewed, UniProtKB/Swiss-Prot B3DPJ1 (SYR_BIFLD)

Names and origin

Protein names

Recommended name:
    Arginyl-tRNA synthetase
    EC=6.1.1.19
Alternative name(s):
    Arginine--tRNA ligase
      Short name=ArgRS

Gene names

Name:	argS
Ordered Locus Names:	BLD_1605

Organism

Bifidobacterium longum (strain DJO10A) [Complete proteome] [HAMAP]

Taxonomic identifier

205913 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	620 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg). HAMAP MF_00123
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP arginine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 620	620	Arginyl-tRNA synthetase HAMAP MF_00123		PRO_1000095334
Regions
	Motif	147 – 157	11	"HIGH" region HAMAP MF_00123

Sequences

	Sequence		Length	Mass (Da)
	B3DPJ1-1 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 14953760A3126BEC	FASTA	620	67,292
	10 20 30 40 50 60 MSPEALSELI SSIAHNLVAA GQAGALTDEL IPPVDKLAVM RPKDRAHGDW ASNIAMQLAK 70 80 90 100 110 120 KAGMKPRDLA EPFAAALAEA DGIAKVEVAG PGFINITLDS ASAAAVVDTV LAAGAMTDTD 130 140 150 160 170 180 KHLNKVNEYG RNAHLGGQTL NLEFVSANPT GPIHIGGTRW AAVGDAMARV LEANGAKVVR 190 200 210 220 230 240 EYYFNDHGEQ INRFAKSLVA AWAEANNLGE AGYQTETPCD GYKGAYINEI AARVQAEAES 250 260 270 280 290 300 DGVDLTALAH QDQGLNDDGE PLGEADTEVR EEFRKRAVPM MFDEIQKSMK DFRVNFDVWF 310 320 330 340 350 360 HENSLYADGK VDAAIEELKS RGDIFDKDGA TWFESTKHGD DKDRVIIKSN GEFAYFAADI 370 380 390 400 410 420 AYYWDKRHRA ENPADVAIYM LGADHHGYIG RMMAMCAAFG DEPGKNMQIL IGQLVNVMKD 430 440 450 460 470 480 GKPVRMSKRA GNVVTIDDLV SVVGVDAARY SLARSDYNQN FDIDLALLAS HTNDNPVYYV 490 500 510 520 530 540 QYAHARSKNV DRNAAVAGIS YEGADLALLD TEADGEVLAA LAQFPSVLAT AADDRQPHKV 550 560 570 580 590 600 ARYLEELAAT YHKWYNVERV VPMALTDPET RGDDEARKAL EIAKNPEPAR AAARLKLNDA 610 620 VQQVIANGLD LLGVTAPEKM

References

[1]

"Comparative genomic analysis of the gut bacterium Bifidobacterium longum reveals loci susceptible to deletion during pure culture growth." Lee J.H., Karamychev V.N., Kozyavkin S.A., Mills D., Pavlov A.R., Pavlova N.V., Polouchine N.N., Richardson P.M., Shakhova V.V., Slesarev A.I., Weimer B., O'Sullivan D.J. BMC Genomics 9:247-247(2008) [PubMed: 18505588] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000605 Genomic DNA. Translation: ACD99050.1.
RefSeq	YP_001955548.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6364074.
GenomeReviews	Gene locus BLD_1605 in contig CP000605_GR.
KEGG	blj:BLD_1605.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	ADHHGYV.
Family and domain databases
HAMAP	MF_00123. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR001278. Arg-tRNA-synth_Ic. IPR015945. Arg-tRNA-synth_Ic_core. IPR005148. Arg-tRNA-synth_Ic_N. IPR008909. DALR_anticod_bd. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.30.1360.70. Arg-tRNA-synth_Ic_N. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11956. Arg_tRNA-synt_1c. 1 hit.
Pfam	PF03485. Arg_tRNA_synt_N. 1 hit. PF05746. DALR_1. 1 hit. PF00750. tRNA-synt_1d. 1 hit. [Graphical view]
PRINTS	PR01038. TRNASYNTHARG.
TIGRFAMs	TIGR00456. argS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYR_BIFLD

Accession

Primary (citable) accession number: B3DPJ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 22, 2008
Last modified:	November 3, 2009
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)