B3DP22 (DNAA_BIFLD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chromosomal replication initiator protein DnaA

Gene names

Name:	dnaA
Ordered Locus Names:	BLD_1436

Organism

Bifidobacterium longum (strain DJO10A) [Complete proteome] [HAMAP]

Taxonomic identifier

205913 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids By similarity. HAMAP MF_00377
Subcellular location	Cytoplasm By similarity HAMAP MF_00377.
Sequence similarities	Belongs to the DnaA family.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA-dependent DNA replication initiation Inferred from electronic annotation. Source: InterPro regulation of DNA replication Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA replication origin binding Inferred from electronic annotation. Source: InterPro nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 500	500	Chromosomal replication initiator protein DnaA HAMAP MF_00377		PRO_1000121949
Regions
	Nucleotide binding	197 – 204	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B3DP22 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 5902D25DEFBF28F5	FASTA	500	55,309
	10 20 30 40 50 60 MVNASGDPVI EAAHIWSDTL TVLKHSASLS PREKGWLEGV VPEGVFGSTI VLCVDNNDTL 70 80 90 100 110 120 QAIQGDLNDS LLQALRTVTG ENMFPAFKVV PKTEPEPLSE AKPAQPYPSE ISPTVAEFGK 130 140 150 160 170 180 ESYGAKPAAA PREPMPATEP QFPVGQQKMN RDPETHLNKN FTFDSFVPGD SNRFARTVAL 190 200 210 220 230 240 AVAEGSGQDF NPLCIYGGSG LGKTHLLNAI GNYALVKDPG LKVRYVTSEE FTNEFIDALQ 250 260 270 280 290 300 NPNQSQGQIA EFNRRYRQVD VLLIDDIQFL GGKEATLDQF FHTFNALHQA NKRIVIASDV 310 320 330 340 350 360 APKNLKGFEA RLISRFESGL TVDVKPPDLE TRIAILRMIA SMNGSKIPSD VLDLIAERFT 370 380 390 400 410 420 ENIRELEGAL TRVTAVASLS NQPVTRALAE QTLQDFFTTD VEIKPTDIIS QVAKYFHLTF 430 440 450 460 470 480 EDLVGKSRTK NVAVPRQIAM YLAREMTSMS LMDIGQVFGG RDHTTVMHAC TRISDRMQQK 490 500 QEIYNYVMEL TVRLKQSNTN

References

[1]

"Comparative genomic analysis of the gut bacterium Bifidobacterium longum reveals loci susceptible to deletion during pure culture growth." Lee J.H., Karamychev V.N., Kozyavkin S.A., Mills D., Pavlov A.R., Pavlova N.V., Polouchine N.N., Richardson P.M., Shakhova V.V., Slesarev A.I., Weimer B., O'Sullivan D.J. BMC Genomics 9:247-247(2008) [PubMed: 18505588] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DJO10A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000605 Genomic DNA. Translation: ACD98881.1.
RefSeq	YP_001955379.1. NC_010816.1.
3D structure databases
ProteinModelPortal	B3DP22.
ModBase	Search...
Protein-protein interaction databases
STRING	B3DP22.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6363161.
GenomeReviews	Gene locus BLD_1436 in contig CP000605_GR.
KEGG	blj:BLD_1436.
PATRIC	21115868. VBIBifLon134794_1453.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG695666.
OMA	MHAYTRI.
ProtClustDB	PRK00149.
Family and domain databases
HAMAP	MF_00377. DnaA_bact. [Tree]
InterPro	IPR003593. ATPase_AAA+_core. IPR001957. Chromosome_initiator_DnaA. IPR020591. Chromosome_initiator_DnaA-like. IPR018312. Chromosome_initiator_DnaA_CS. IPR013159. DnaA_C. IPR013317. DnaA_Hda. IPR010921. Trp_repressor/repl_initiator. [Graphical view]
Gene3D	G3DSA:1.10.1750.10. DnaA_C. 1 hit.
KO	K02313.
Pfam	PF00308. Bac_DnaA. 1 hit. PF08299. Bac_DnaA_C. 1 hit. [Graphical view]
PRINTS	PR00051. DNAA.
SMART	SM00382. AAA. 1 hit. SM00760. Bac_DnaA_C. 1 hit. [Graphical view]
SUPFAM	SSF48295. Trp_repress_rep. 1 hit.
TIGRFAMs	TIGR00362. DnaA. 1 hit.
PROSITE	PS01008. DNAA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNAA_BIFLD

Accession

Primary (citable) accession number: B3DP22

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 22, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program