Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

POU domain, class 5, transcription factor 1.1

Gene

pou5f1.1

Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Activates transcription when directly bound to the octamer DNA sequence, but can form repression complexes with other proteins at the promoter site to inhibit transcription. Binds to the promoter of the vent2-B gene to activate transcription when in the presence of other BMP signaling factors also bound to the promoter. Inhibits the competence of ectodermal cells to respond to BMP during embryogenesis thereby inhibiting epidermal differentiation and promoting neural induction. Antagonizes the activity of nodal/activin signaling by forming a transcriptional repression complex on the gsc and mix2 gene promoters to inhibit their transcription, and thus maintain the undifferentiated state of embryonic cells to prevent them from differentiating prematurely. Acts maternally to inhibit vegt and beta-catenin-activated gene transcription by forming a transcriptional repression complex on the nodal/nr1 and siamois promoters to inhibit their transcription (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi326 – 385HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processDifferentiation, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 5, transcription factor 1.1
Alternative name(s):
POU class V protein oct-25By similarity
Gene namesi
Name:pou5f1.1Imported
Synonyms:oct25By similarity
OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Taxonomic identifieri8364 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
Proteomesi
  • UP000008143 Componenti: Unassembled WGS sequence

Organism-specific databases

XenbaseiXB-GENE-919841. pou5f3.2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003904961 – 459POU domain, class 5, transcription factor 1.1Add BLAST459

Proteomic databases

PaxDbiB3DM25.

Interactioni

Subunit structurei

Interacts with components of the transcription complex that assembles on the vent2-B gene, including vent2 (via C-terminus), smad1 and smad4. Forms a repression complex on the promoters of the gsc and mix2 genes via interactions with the nodal/activin signaling pathway transducers foxh1/fast1, gtf2ird1/wbscr11 and smad2. Forms a repression complex on the promoters of the nodal/nr1 and siamois genes with the maternal factors tcf7l1/tcf3 and vegt (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi8364.ENSXETP00000008656.

Structurei

3D structure databases

SMRiB3DM25.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini232 – 306POU-specificPROSITE-ProRule annotationAdd BLAST75

Sequence similaritiesi

Belongs to the POU transcription factor family. Class-5 subfamily.Sequence analysis

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG3802. Eukaryota.
ENOG410XQ7X. LUCA.
HOGENOMiHOG000063726.
HOVERGENiHBG053782.
InParanoidiB3DM25.
KOiK09367.
OrthoDBiEOG091G0U0S.
TreeFamiTF316413.

Family and domain databases

CDDicd00086. homeodomain. 1 hit.
InterProiView protein in InterPro
IPR009057. Homeobox-like_sf.
IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR010982. Lambda_DNA-bd_dom_sf.
IPR013847. POU.
IPR000327. POU_dom.
IPR015585. POU_dom_5.
PANTHERiPTHR11636:SF86. PTHR11636:SF86. 1 hit.
PfamiView protein in Pfam
PF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
PRINTSiPR00028. POUDOMAIN.
SMARTiView protein in SMART
SM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiView protein in PROSITE
PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.

Sequencei

Sequence statusi: Complete.

B3DM25-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYSQQPFPPF AFNAGLIQDP ANCHFGGYTG LGHPQPFSFA FSTLKSENGD
60 70 80 90 100
SGVQGMGDCS APVMPWNSMA SFDHHGQVET NQQGNPIRAP SPTPTLSDSR
110 120 130 140 150
IKVKEEVAHE TDSGEESPEP KYPSPPNPSL YYPNTWTGSP FWQVNPTAGN
160 170 180 190 200
NSNSTNPMPS QTLVKNGSLP GNTTYPTPAN QSPNTPVDCV VSSMESSRCS
210 220 230 240 250
SANSSNGAIN ERATTIPNGG MVDGGQSSDN EEEVPSESEM EQFAKDLKHK
260 270 280 290 300
RVSMGYTQAD VGYALGVLYG KMFSQTTICR FESLQLSFKN MCQLKPFLER
310 320 330 340 350
WLVEAENNDN LQELINREQV IAQTRKRKRR TNIENIVKGT LESYFMKCSK
360 370 380 390 400
PGAQEMVQIA KELNMDKDVV RVWFCNRRQK GKRQGMPTVD ENDGEGYDVG
410 420 430 440 450
QTMASPPVGH YSLPQVVTSQ GYMAAPLGST PPLYASAFHK NELFPQPLPH

AMPMGGHIG
Length:459
Mass (Da):50,320
Last modified:July 22, 2008 - v1
Checksum:i1CB1FA5F6F0D39C8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti17I → M in AAI67702 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC167674 mRNA. Translation: AAI67674.1.
BC167702 mRNA. Translation: AAI67702.1.
RefSeqiNP_001123406.1. NM_001129934.1.
UniGeneiStr.6229.

Genome annotation databases

GeneIDi100170181.
KEGGixtr:100170181.

Similar proteinsi

Entry informationi

Entry nameiP5F11_XENTR
AccessioniPrimary (citable) accession number: B3DM25
Secondary accession number(s): B3DM50
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: July 22, 2008
Last modified: November 22, 2017
This is version 65 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families