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B3DM25

- P5F11_XENTR

UniProt

B3DM25 - P5F11_XENTR

Protein

POU domain, class 5, transcription factor 1.1

Gene

pou5f1.1

Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 43 (01 Oct 2014)
      Sequence version 1 (22 Jul 2008)
      Previous versions | rss
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    Functioni

    Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Activates transcription when directly bound to the octamer DNA sequence, but can form repression complexes with other proteins at the promoter site to inhibit transcription. Binds to the promoter of the vent2-B gene to activate transcription when in the presence of other BMP signaling factors also bound to the promoter. Inhibits the competence of ectodermal cells to respond to BMP during embryogenesis thereby inhibiting epidermal differentiation and promoting neural induction. Antagonizes the activity of nodal/activin signaling by forming a transcriptional repression complex on the gsc and mix2 gene promoters to inhibit their transcription, and thus maintain the undifferentiated state of embryonic cells to prevent them from differentiating prematurely. Acts maternally to inhibit vegt and beta-catenin-activated gene transcription by forming a transcriptional repression complex on the nodal/nr1 and siamois promoters to inhibit their transcription By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi326 – 38560HomeoboxPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. sequence-specific DNA binding Source: UniProtKB
    2. sequence-specific DNA binding transcription factor activity Source: InterPro
    3. SMAD binding Source: UniProtKB
    4. transcription factor binding Source: UniProtKB
    5. transcription regulatory region DNA binding Source: UniProtKB

    GO - Biological processi

    1. gastrulation Source: UniProtKB
    2. negative regulation of activin receptor signaling pathway Source: UniProtKB
    3. negative regulation of BMP signaling pathway Source: UniProtKB
    4. negative regulation of epidermal cell differentiation Source: UniProtKB
    5. negative regulation of transcription, DNA-templated Source: UniProtKB
    6. positive regulation of transcription, DNA-templated Source: UniProtKB
    7. regulation of neurogenesis Source: UniProtKB
    8. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Developmental protein

    Keywords - Biological processi

    Differentiation, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    POU domain, class 5, transcription factor 1.1
    Alternative name(s):
    POU class V protein oct-25By similarity
    Gene namesi
    Name:pou5f1.1Imported
    Synonyms:oct25By similarity
    OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
    Taxonomic identifieri8364 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
    ProteomesiUP000008143: Unplaced

    Organism-specific databases

    XenbaseiXB-GENE-919841. pou5f3.2.

    Subcellular locationi

    Nucleus By similarityPROSITE-ProRule annotation

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell
    2. transcription factor complex Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 459459POU domain, class 5, transcription factor 1.1PRO_0000390496Add
    BLAST

    Expressioni

    Gene expression databases

    BgeeiB3DM25.

    Interactioni

    Subunit structurei

    Interacts with components of the transcription complex that assembles on the vent2-B gene, including vent2 (via C-terminus), smad1 and smad4. Forms a repression complex on the promoters of the gsc and mix2 genes via interactions with the nodal/activin signaling pathway transducers foxh1/fast1, gtf2ird1/wbscr11 and smad2. Forms a repression complex on the promoters of the nodal/nr1 and siamois genes with the maternal factors tcf7l1/tcf3 and vegt By similarity.By similarity

    Protein-protein interaction databases

    STRINGi8364.ENSXETP00000008656.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini232 – 30675POU-specificPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the POU transcription factor family. Class-5 subfamily.Sequence Analysis
    Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
    Contains 1 POU-specific domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Homeobox

    Phylogenomic databases

    eggNOGiNOG329627.
    GeneTreeiENSGT00750000117482.
    HOGENOMiHOG000063726.
    HOVERGENiHBG053782.
    OrthoDBiEOG77DJ5X.
    TreeFamiTF316413.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    1.10.260.40. 1 hit.
    InterProiIPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR010982. Lambda_DNA-bd_dom.
    IPR013847. POU.
    IPR000327. POU_specific.
    [Graphical view]
    PfamiPF00046. Homeobox. 1 hit.
    PF00157. Pou. 1 hit.
    [Graphical view]
    PRINTSiPR00028. POUDOMAIN.
    SMARTiSM00389. HOX. 1 hit.
    SM00352. POU. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    SSF47413. SSF47413. 1 hit.
    PROSITEiPS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    PS00465. POU_2. 1 hit.
    PS51179. POU_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B3DM25-1 [UniParc]FASTAAdd to Basket

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    MYSQQPFPPF AFNAGLIQDP ANCHFGGYTG LGHPQPFSFA FSTLKSENGD    50
    SGVQGMGDCS APVMPWNSMA SFDHHGQVET NQQGNPIRAP SPTPTLSDSR 100
    IKVKEEVAHE TDSGEESPEP KYPSPPNPSL YYPNTWTGSP FWQVNPTAGN 150
    NSNSTNPMPS QTLVKNGSLP GNTTYPTPAN QSPNTPVDCV VSSMESSRCS 200
    SANSSNGAIN ERATTIPNGG MVDGGQSSDN EEEVPSESEM EQFAKDLKHK 250
    RVSMGYTQAD VGYALGVLYG KMFSQTTICR FESLQLSFKN MCQLKPFLER 300
    WLVEAENNDN LQELINREQV IAQTRKRKRR TNIENIVKGT LESYFMKCSK 350
    PGAQEMVQIA KELNMDKDVV RVWFCNRRQK GKRQGMPTVD ENDGEGYDVG 400
    QTMASPPVGH YSLPQVVTSQ GYMAAPLGST PPLYASAFHK NELFPQPLPH 450
    AMPMGGHIG 459
    Length:459
    Mass (Da):50,320
    Last modified:July 22, 2008 - v1
    Checksum:i1CB1FA5F6F0D39C8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti17 – 171I → M in AAI67702. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC167674 mRNA. Translation: AAI67674.1.
    BC167702 mRNA. Translation: AAI67702.1.
    RefSeqiNP_001123406.1. NM_001129934.1.
    UniGeneiStr.6229.

    Genome annotation databases

    EnsembliENSXETT00000008656; ENSXETP00000008656; ENSXETG00000003991.
    GeneIDi100170181.
    KEGGixtr:100170181.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC167674 mRNA. Translation: AAI67674.1 .
    BC167702 mRNA. Translation: AAI67702.1 .
    RefSeqi NP_001123406.1. NM_001129934.1.
    UniGenei Str.6229.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 8364.ENSXETP00000008656.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSXETT00000008656 ; ENSXETP00000008656 ; ENSXETG00000003991 .
    GeneIDi 100170181.
    KEGGi xtr:100170181.

    Organism-specific databases

    CTDi 379522.
    Xenbasei XB-GENE-919841. pou5f3.2.

    Phylogenomic databases

    eggNOGi NOG329627.
    GeneTreei ENSGT00750000117482.
    HOGENOMi HOG000063726.
    HOVERGENi HBG053782.
    OrthoDBi EOG77DJ5X.
    TreeFami TF316413.

    Gene expression databases

    Bgeei B3DM25.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    1.10.260.40. 1 hit.
    InterProi IPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR010982. Lambda_DNA-bd_dom.
    IPR013847. POU.
    IPR000327. POU_specific.
    [Graphical view ]
    Pfami PF00046. Homeobox. 1 hit.
    PF00157. Pou. 1 hit.
    [Graphical view ]
    PRINTSi PR00028. POUDOMAIN.
    SMARTi SM00389. HOX. 1 hit.
    SM00352. POU. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    SSF47413. SSF47413. 1 hit.
    PROSITEi PS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    PS00465. POU_2. 1 hit.
    PS51179. POU_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Xenopus Gene Collection (XGC) project
      Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: GastrulaImported and NeurulaImported.

    Entry informationi

    Entry nameiP5F11_XENTR
    AccessioniPrimary (citable) accession number: B3DM25
    Secondary accession number(s): B3DM50
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 2009
    Last sequence update: July 22, 2008
    Last modified: October 1, 2014
    This is version 43 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3