Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

B3DM25

- P5F11_XENTR

UniProt

B3DM25 - P5F11_XENTR

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

POU domain, class 5, transcription factor 1.1

Gene

pou5f1.1

Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Activates transcription when directly bound to the octamer DNA sequence, but can form repression complexes with other proteins at the promoter site to inhibit transcription. Binds to the promoter of the vent2-B gene to activate transcription when in the presence of other BMP signaling factors also bound to the promoter. Inhibits the competence of ectodermal cells to respond to BMP during embryogenesis thereby inhibiting epidermal differentiation and promoting neural induction. Antagonizes the activity of nodal/activin signaling by forming a transcriptional repression complex on the gsc and mix2 gene promoters to inhibit their transcription, and thus maintain the undifferentiated state of embryonic cells to prevent them from differentiating prematurely. Acts maternally to inhibit vegt and beta-catenin-activated gene transcription by forming a transcriptional repression complex on the nodal/nr1 and siamois promoters to inhibit their transcription (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi326 – 38560HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. sequence-specific DNA binding Source: UniProtKB
  2. sequence-specific DNA binding transcription factor activity Source: InterPro
  3. SMAD binding Source: UniProtKB
  4. transcription factor binding Source: UniProtKB
  5. transcription regulatory region DNA binding Source: UniProtKB

GO - Biological processi

  1. gastrulation Source: UniProtKB
  2. negative regulation of activin receptor signaling pathway Source: UniProtKB
  3. negative regulation of BMP signaling pathway Source: UniProtKB
  4. negative regulation of epidermal cell differentiation Source: UniProtKB
  5. negative regulation of transcription, DNA-templated Source: UniProtKB
  6. positive regulation of transcription, DNA-templated Source: UniProtKB
  7. regulation of neurogenesis Source: UniProtKB
  8. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Differentiation, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_232603. Transcriptional regulation of pluripotent stem cells.

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 5, transcription factor 1.1
Alternative name(s):
POU class V protein oct-25By similarity
Gene namesi
Name:pou5f1.1Imported
Synonyms:oct25By similarity
OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Taxonomic identifieri8364 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
ProteomesiUP000008143: Unplaced

Organism-specific databases

XenbaseiXB-GENE-919841. pou5f3.2.

Subcellular locationi

Nucleus By similarityPROSITE-ProRule annotation

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
  2. transcription factor complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 459459POU domain, class 5, transcription factor 1.1PRO_0000390496Add
BLAST

Expressioni

Gene expression databases

BgeeiB3DM25.

Interactioni

Subunit structurei

Interacts with components of the transcription complex that assembles on the vent2-B gene, including vent2 (via C-terminus), smad1 and smad4. Forms a repression complex on the promoters of the gsc and mix2 genes via interactions with the nodal/activin signaling pathway transducers foxh1/fast1, gtf2ird1/wbscr11 and smad2. Forms a repression complex on the promoters of the nodal/nr1 and siamois genes with the maternal factors tcf7l1/tcf3 and vegt (By similarity).By similarity

Protein-protein interaction databases

STRINGi8364.ENSXETP00000008656.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini232 – 30675POU-specificPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the POU transcription factor family. Class-5 subfamily.Sequence Analysis
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
Contains 1 POU-specific domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG329627.
GeneTreeiENSGT00760000118935.
HOGENOMiHOG000063726.
HOVERGENiHBG053782.
InParanoidiB3DM25.
OrthoDBiEOG77DJ5X.
TreeFamiTF316413.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B3DM25-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYSQQPFPPF AFNAGLIQDP ANCHFGGYTG LGHPQPFSFA FSTLKSENGD
60 70 80 90 100
SGVQGMGDCS APVMPWNSMA SFDHHGQVET NQQGNPIRAP SPTPTLSDSR
110 120 130 140 150
IKVKEEVAHE TDSGEESPEP KYPSPPNPSL YYPNTWTGSP FWQVNPTAGN
160 170 180 190 200
NSNSTNPMPS QTLVKNGSLP GNTTYPTPAN QSPNTPVDCV VSSMESSRCS
210 220 230 240 250
SANSSNGAIN ERATTIPNGG MVDGGQSSDN EEEVPSESEM EQFAKDLKHK
260 270 280 290 300
RVSMGYTQAD VGYALGVLYG KMFSQTTICR FESLQLSFKN MCQLKPFLER
310 320 330 340 350
WLVEAENNDN LQELINREQV IAQTRKRKRR TNIENIVKGT LESYFMKCSK
360 370 380 390 400
PGAQEMVQIA KELNMDKDVV RVWFCNRRQK GKRQGMPTVD ENDGEGYDVG
410 420 430 440 450
QTMASPPVGH YSLPQVVTSQ GYMAAPLGST PPLYASAFHK NELFPQPLPH

AMPMGGHIG
Length:459
Mass (Da):50,320
Last modified:July 22, 2008 - v1
Checksum:i1CB1FA5F6F0D39C8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti17 – 171I → M in AAI67702. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC167674 mRNA. Translation: AAI67674.1.
BC167702 mRNA. Translation: AAI67702.1.
RefSeqiNP_001123406.1. NM_001129934.1.
UniGeneiStr.6229.

Genome annotation databases

EnsembliENSXETT00000008656; ENSXETP00000008656; ENSXETG00000003991.
GeneIDi100170181.
KEGGixtr:100170181.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC167674 mRNA. Translation: AAI67674.1 .
BC167702 mRNA. Translation: AAI67702.1 .
RefSeqi NP_001123406.1. NM_001129934.1.
UniGenei Str.6229.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 8364.ENSXETP00000008656.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSXETT00000008656 ; ENSXETP00000008656 ; ENSXETG00000003991 .
GeneIDi 100170181.
KEGGi xtr:100170181.

Organism-specific databases

CTDi 379522.
Xenbasei XB-GENE-919841. pou5f3.2.

Phylogenomic databases

eggNOGi NOG329627.
GeneTreei ENSGT00760000118935.
HOGENOMi HOG000063726.
HOVERGENi HBG053782.
InParanoidi B3DM25.
OrthoDBi EOG77DJ5X.
TreeFami TF316413.

Enzyme and pathway databases

Reactomei REACT_232603. Transcriptional regulation of pluripotent stem cells.

Gene expression databases

Bgeei B3DM25.

Family and domain databases

Gene3Di 1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProi IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
[Graphical view ]
Pfami PF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view ]
PRINTSi PR00028. POUDOMAIN.
SMARTi SM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEi PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Xenopus Gene Collection (XGC) project
    Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: GastrulaImported and NeurulaImported.

Entry informationi

Entry nameiP5F11_XENTR
AccessioniPrimary (citable) accession number: B3DM25
Secondary accession number(s): B3DM50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: July 22, 2008
Last modified: November 26, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3