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Protein

POU domain, class 5, transcription factor 1.1

Gene

pou5f1.1

Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Activates transcription when directly bound to the octamer DNA sequence, but can form repression complexes with other proteins at the promoter site to inhibit transcription. Binds to the promoter of the vent2-B gene to activate transcription when in the presence of other BMP signaling factors also bound to the promoter. Inhibits the competence of ectodermal cells to respond to BMP during embryogenesis thereby inhibiting epidermal differentiation and promoting neural induction. Antagonizes the activity of nodal/activin signaling by forming a transcriptional repression complex on the gsc and mix2 gene promoters to inhibit their transcription, and thus maintain the undifferentiated state of embryonic cells to prevent them from differentiating prematurely. Acts maternally to inhibit vegt and beta-catenin-activated gene transcription by forming a transcriptional repression complex on the nodal/nr1 and siamois promoters to inhibit their transcription (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi326 – 38560HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. sequence-specific DNA binding Source: UniProtKB
  2. sequence-specific DNA binding transcription factor activity Source: InterPro
  3. SMAD binding Source: UniProtKB
  4. transcription factor binding Source: UniProtKB
  5. transcription regulatory region DNA binding Source: UniProtKB

GO - Biological processi

  1. gastrulation Source: UniProtKB
  2. negative regulation of activin receptor signaling pathway Source: UniProtKB
  3. negative regulation of BMP signaling pathway Source: UniProtKB
  4. negative regulation of epidermal cell differentiation Source: UniProtKB
  5. negative regulation of transcription, DNA-templated Source: UniProtKB
  6. positive regulation of transcription, DNA-templated Source: UniProtKB
  7. regulation of neurogenesis Source: UniProtKB
  8. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Differentiation, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_329763. Transcriptional regulation of pluripotent stem cells.

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 5, transcription factor 1.1
Alternative name(s):
POU class V protein oct-25By similarity
Gene namesi
Name:pou5f1.1Imported
Synonyms:oct25By similarity
OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Taxonomic identifieri8364 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
ProteomesiUP000008143 Componenti: Unplaced

Organism-specific databases

XenbaseiXB-GENE-919841. pou5f3.2.

Subcellular locationi

Nucleus PROSITE-ProRule annotationBy similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
  2. transcription factor complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 459459POU domain, class 5, transcription factor 1.1PRO_0000390496Add
BLAST

Expressioni

Gene expression databases

BgeeiB3DM25.

Interactioni

Subunit structurei

Interacts with components of the transcription complex that assembles on the vent2-B gene, including vent2 (via C-terminus), smad1 and smad4. Forms a repression complex on the promoters of the gsc and mix2 genes via interactions with the nodal/activin signaling pathway transducers foxh1/fast1, gtf2ird1/wbscr11 and smad2. Forms a repression complex on the promoters of the nodal/nr1 and siamois genes with the maternal factors tcf7l1/tcf3 and vegt (By similarity).By similarity

Protein-protein interaction databases

STRINGi8364.ENSXETP00000008656.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini232 – 30675POU-specificPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the POU transcription factor family. Class-5 subfamily.Sequence Analysis
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
Contains 1 POU-specific domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG329627.
HOGENOMiHOG000063726.
HOVERGENiHBG053782.
InParanoidiB3DM25.
OrthoDBiEOG77DJ5X.
TreeFamiTF316413.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B3DM25-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYSQQPFPPF AFNAGLIQDP ANCHFGGYTG LGHPQPFSFA FSTLKSENGD
60 70 80 90 100
SGVQGMGDCS APVMPWNSMA SFDHHGQVET NQQGNPIRAP SPTPTLSDSR
110 120 130 140 150
IKVKEEVAHE TDSGEESPEP KYPSPPNPSL YYPNTWTGSP FWQVNPTAGN
160 170 180 190 200
NSNSTNPMPS QTLVKNGSLP GNTTYPTPAN QSPNTPVDCV VSSMESSRCS
210 220 230 240 250
SANSSNGAIN ERATTIPNGG MVDGGQSSDN EEEVPSESEM EQFAKDLKHK
260 270 280 290 300
RVSMGYTQAD VGYALGVLYG KMFSQTTICR FESLQLSFKN MCQLKPFLER
310 320 330 340 350
WLVEAENNDN LQELINREQV IAQTRKRKRR TNIENIVKGT LESYFMKCSK
360 370 380 390 400
PGAQEMVQIA KELNMDKDVV RVWFCNRRQK GKRQGMPTVD ENDGEGYDVG
410 420 430 440 450
QTMASPPVGH YSLPQVVTSQ GYMAAPLGST PPLYASAFHK NELFPQPLPH

AMPMGGHIG
Length:459
Mass (Da):50,320
Last modified:July 21, 2008 - v1
Checksum:i1CB1FA5F6F0D39C8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti17 – 171I → M in AAI67702 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC167674 mRNA. Translation: AAI67674.1.
BC167702 mRNA. Translation: AAI67702.1.
RefSeqiNP_001123406.1. NM_001129934.1.
UniGeneiStr.6229.

Genome annotation databases

GeneIDi100170181.
KEGGixtr:100170181.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC167674 mRNA. Translation: AAI67674.1.
BC167702 mRNA. Translation: AAI67702.1.
RefSeqiNP_001123406.1. NM_001129934.1.
UniGeneiStr.6229.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi8364.ENSXETP00000008656.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100170181.
KEGGixtr:100170181.

Organism-specific databases

CTDi379522.
XenbaseiXB-GENE-919841. pou5f3.2.

Phylogenomic databases

eggNOGiNOG329627.
HOGENOMiHOG000063726.
HOVERGENiHBG053782.
InParanoidiB3DM25.
OrthoDBiEOG77DJ5X.
TreeFamiTF316413.

Enzyme and pathway databases

ReactomeiREACT_329763. Transcriptional regulation of pluripotent stem cells.

Gene expression databases

BgeeiB3DM25.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Xenopus Gene Collection (XGC) project
    Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: GastrulaImported and NeurulaImported.

Entry informationi

Entry nameiP5F11_XENTR
AccessioniPrimary (citable) accession number: B3DM25
Secondary accession number(s): B3DM50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 14, 2009
Last sequence update: July 21, 2008
Last modified: March 31, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.