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B3DM25 (P5F11_XENTR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
POU domain, class 5, transcription factor 1.1
Alternative name(s):
POU class V protein oct-25
Gene names
Name:pou5f1.1
Synonyms:oct25
OrganismXenopus tropicalis (Western clawed frog) (Silurana tropicalis) [Reference proteome]
Taxonomic identifier8364 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana

Protein attributes

Sequence length459 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Activates transcription when directly bound to the octamer DNA sequence, but can form repression complexes with other proteins at the promoter site to inhibit transcription. Binds to the promoter of the vent2-B gene to activate transcription when in the presence of other BMP signaling factors also bound to the promoter. Inhibits the competence of ectodermal cells to respond to BMP during embryogenesis thereby inhibiting epidermal differentiation and promoting neural induction. Antagonizes the activity of nodal/activin signaling by forming a transcriptional repression complex on the gsc and mix2 gene promoters to inhibit their transcription, and thus maintain the undifferentiated state of embryonic cells to prevent them from differentiating prematurely. Acts maternally to inhibit vegt and beta-catenin-activated gene transcription by forming a transcriptional repression complex on the nodal/nr1 and siamois promoters to inhibit their transcription By similarity. UniProtKB Q7T103

Subunit structure

Interacts with components of the transcription complex that assembles on the vent2-B gene, including vent2 (via C-terminus), smad1 and smad4. Forms a repression complex on the promoters of the gsc and mix2 genes via interactions with the nodal/activin signaling pathway transducers foxh1/fast1, gtf2ird1/wbscr11 and smad2. Forms a repression complex on the promoters of the nodal/nr1 and siamois genes with the maternal factors tcf7l1/tcf3 and vegt By similarity. UniProtKB Q7T103

Subcellular location

Nucleus By similarity UniProtKB Q01860.

Sequence similarities

Belongs to the POU transcription factor family. Class-5 subfamily.

Contains 1 homeobox DNA-binding domain.

Contains 1 POU-specific domain.

Ontologies

Keywords
   Biological processDifferentiation
Transcription
Transcription regulation
   Cellular componentNucleus
   DomainHomeobox
   LigandDNA-binding
   Molecular functionActivator
Developmental protein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processgastrulation

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of BMP signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of activin receptor signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of epidermal cell differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of transcription, DNA-templated

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of transcription, DNA-templated

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of neurogenesis

Inferred from sequence or structural similarity. Source: UniProtKB

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

transcription factor complex

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionSMAD binding

Inferred from sequence or structural similarity. Source: UniProtKB

sequence-specific DNA binding

Inferred from sequence or structural similarity. Source: UniProtKB

sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: InterPro

transcription factor binding

Inferred from sequence or structural similarity. Source: UniProtKB

transcription regulatory region DNA binding

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 459459POU domain, class 5, transcription factor 1.1
PRO_0000390496

Regions

Domain232 – 30675POU-specific
DNA binding326 – 38560Homeobox

Experimental info

Sequence conflict171I → M in AAI67702. Ref.1

Sequences

Sequence LengthMass (Da)Tools
B3DM25 [UniParc].

Last modified July 22, 2008. Version 1.
Checksum: 1CB1FA5F6F0D39C8

FASTA45950,320
        10         20         30         40         50         60 
MYSQQPFPPF AFNAGLIQDP ANCHFGGYTG LGHPQPFSFA FSTLKSENGD SGVQGMGDCS 

        70         80         90        100        110        120 
APVMPWNSMA SFDHHGQVET NQQGNPIRAP SPTPTLSDSR IKVKEEVAHE TDSGEESPEP 

       130        140        150        160        170        180 
KYPSPPNPSL YYPNTWTGSP FWQVNPTAGN NSNSTNPMPS QTLVKNGSLP GNTTYPTPAN 

       190        200        210        220        230        240 
QSPNTPVDCV VSSMESSRCS SANSSNGAIN ERATTIPNGG MVDGGQSSDN EEEVPSESEM 

       250        260        270        280        290        300 
EQFAKDLKHK RVSMGYTQAD VGYALGVLYG KMFSQTTICR FESLQLSFKN MCQLKPFLER 

       310        320        330        340        350        360 
WLVEAENNDN LQELINREQV IAQTRKRKRR TNIENIVKGT LESYFMKCSK PGAQEMVQIA 

       370        380        390        400        410        420 
KELNMDKDVV RVWFCNRRQK GKRQGMPTVD ENDGEGYDVG QTMASPPVGH YSLPQVVTSQ 

       430        440        450 
GYMAAPLGST PPLYASAFHK NELFPQPLPH AMPMGGHIG 

« Hide

References

[1]NIH - Xenopus Gene Collection (XGC) project
Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Gastrula and Neurula.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC167674 mRNA. Translation: AAI67674.1.
BC167702 mRNA. Translation: AAI67702.1.
RefSeqNP_001123406.1. NM_001129934.1.
UniGeneStr.6229.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING8364.ENSXETP00000008656.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSXETT00000008656; ENSXETP00000008656; ENSXETG00000003991.
GeneID100170181.
KEGGxtr:100170181.

Organism-specific databases

CTD379522.
XenbaseXB-GENE-919841. pou5f3.2.

Phylogenomic databases

eggNOGNOG329627.
GeneTreeENSGT00750000117482.
HOGENOMHOG000063726.
HOVERGENHBG053782.
OrthoDBEOG77DJ5X.
TreeFamTF316413.

Gene expression databases

BgeeB3DM25.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSPR00028. POUDOMAIN.
SMARTSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameP5F11_XENTR
AccessionPrimary (citable) accession number: B3DM25
Secondary accession number(s): B3DM50
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: July 22, 2008
Last modified: April 16, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families