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B3DK56

- PTPRU_DANRE

UniProt

B3DK56 - PTPRU_DANRE

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Protein

Receptor-type tyrosine-protein phosphatase U

Gene
ptpru, rptppsi
Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Tyrosine-protein phosphatase which dephosphorylates CTNNB1. May function in cell proliferation and migration and play a role in the maintenance of epithelial integrity. Functions in somitogenesis. Functions as a regulator of the biochemical clock responsible for the segmentation of the presomitic mesoderm.1 Publication

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei1051 – 10511Substrate Reviewed prediction
Active sitei1083 – 10831Phosphocysteine intermediate By similarity
Binding sitei1127 – 11271Substrate By similarity
Active sitei1378 – 13781Phosphocysteine intermediate By similarity

GO - Molecular functioni

  1. protein tyrosine phosphatase activity Source: UniProtKB-EC

GO - Biological processi

  1. cell adhesion Source: UniProtKB-KW
  2. cell differentiation Source: UniProtKB-KW
  3. somite specification Source: ZFIN
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase, Protein phosphatase, Receptor

Keywords - Biological processi

Cell adhesion, Differentiation

Names & Taxonomyi

Protein namesi
Recommended name:
Receptor-type tyrosine-protein phosphatase U (EC:3.1.3.48)
Short name:
R-PTP-U
Alternative name(s):
Receptor-type protein-tyrosine phosphatase psi
Short name:
R-PTP-psi
Gene namesi
Name:ptpru
Synonyms:rptppsi
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437: Unplaced

Organism-specific databases

ZFINiZDB-GENE-030131-7036. ptprub.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini17 – 748732Extracellular Reviewed predictionAdd
BLAST
Transmembranei749 – 76921Helical; Reviewed predictionAdd
BLAST
Topological domaini770 – 1444675Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. cell junction Source: UniProtKB-SubCell
  2. integral component of membrane Source: ZFIN
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616 Reviewed predictionAdd
BLAST
Chaini17 – 14441428Receptor-type tyrosine-protein phosphatase UPRO_0000371661Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi73 – 731N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi206 ↔ 260 Reviewed prediction
Glycosylationi406 – 4061N-linked (GlcNAc...) Reviewed prediction
Glycosylationi684 – 6841N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Developmental stagei

First detected in somites, pronephric duct, midbrain hindbrain boundary, otic vesicle and retina 10-24 after fertilization. When somite formation is complete expression becomes restricted to the retina, the forebrain midbrain, the midbrain hindbrain boundary, the otic vesicle and the branchial arches.1 Publication

Structurei

3D structure databases

ProteinModelPortaliB3DK56.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 184161MAMAdd
BLAST
Domaini186 – 27186Ig-like C2-typeAdd
BLAST
Domaini284 – 37996Fibronectin type-III 1Add
BLAST
Domaini382 – 483102Fibronectin type-III 2Add
BLAST
Domaini489 – 590102Fibronectin type-III 3Add
BLAST
Domaini597 – 67781Fibronectin type-III 4Add
BLAST
Domaini886 – 1142257Tyrosine-protein phosphatase 1Add
BLAST
Domaini1174 – 1437264Tyrosine-protein phosphatase 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1083 – 10897Substrate binding By similarity

Sequence similaritiesi

Contains 1 MAM domain.

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG311686.
HOGENOMiHOG000049029.
InParanoidiQ6E5N7.
KOiK16662.
PhylomeDBiB3DK56.

Family and domain databases

Gene3Di2.60.40.10. 4 hits.
3.90.190.10. 2 hits.
InterProiIPR008985. ConA-like_lec_gl_sf.
IPR003961. Fibronectin_type3.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR000998. MAM_dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
[Graphical view]
PfamiPF00041. fn3. 1 hit.
PF00629. MAM. 1 hit.
PF00102. Y_phosphatase. 2 hits.
[Graphical view]
PRINTSiPR00020. MAMDOMAIN.
PR00700. PRTYPHPHTASE.
SMARTiSM00060. FN3. 3 hits.
SM00409. IG. 1 hit.
SM00137. MAM. 1 hit.
SM00194. PTPc. 2 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 2 hits.
SSF49899. SSF49899. 1 hit.
SSF52799. SSF52799. 2 hits.
PROSITEiPS50853. FN3. 3 hits.
PS00740. MAM_1. 1 hit.
PS50060. MAM_2. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 2 hits.
PS50056. TYR_PHOSPHATASE_2. 2 hits.
PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: B3DK56-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MNTCAFLLIL AVQIHADSVE GPTAGCTFDE DSDPSLCEFS QGEEDDFDWQ     50
LFRAHASPHS TSDLLRGSYM MVNSSQHAAG QRAQLLLQTL SENDTHCVQF 100
SYFLYSRDGH SPGALRVYVR VNGGPLGIPV WNVSGSRGRQ WHQVELAVST 150
FWPNEYQILL EATVDRKGYI AVDDILLLNY PCYKAPHFSR LGDVEVNAGQ 200
NATFQCVAAG RPSEAEKFLL ERHNGEVSSG GSVKHLGRNR FAVSFQLEDV 250
QKPEQDLYRC VTQSSRGSGV SNFAELIVKV PPSPIAPPQL LRAGSTYLII 300
QLNTNSILGD GPIIRREIEY RASLAPWSEI LGVNMVTYKL WHLDPDTEYH 350
ISVLLTRPGE GGTGPPGPPL ISRTKCAEPM RALRGLRASE IQSRQLTLQW 400
EVPAFNLTRC HTYSVSLCYR YTTAGGGGGH NTTVRECLAV EHNTSRFTLR 450
DLPPFHTIQI RLALANTEGK KEGKEVMFQT EEDIPGGIAP ESLTFTPLED 500
MIFLKWEEPV EPNGLITQYE ISYQSIESSD PGINVPGPRR TVSKLKNETY 550
HMFSNLHPGT TYLISVRART AKGFGQTALT EITTNISAPT FDYGDMPSPL 600
SETENTITVL LRPAQGRGAP VSTYQVVVEE EAGRKVKREL GIQDCFPIPT 650
SHGEAQARGA PHYYTAELPP SSLSEATPFT VGDNHTYNGY WNSPLDPRKN 700
YLVYFQAMSN FRGETRINCI RIARKAACKD HQRALEVTQR SEEMGLILGV 750
CAGGLVVLIL LLGAIIIIIK KGRDYYSYSY YPRKPGNMNK TPITYRQEKS 800
NMMGSMERSF TDQSTLQEDE RMALSFMDTH TCSTRSDPRS SMNESSSLLG 850
GSPRRQCGRK GSPYHTGQLH PAVRVADLLQ HINQMKTAEG YGFKQEYESF 900
FDGWDINKKK DKTKGRHDTL MGYDRHRVKL HPLLGDPNSD YINANYIDGY 950
HRSNHFIATQ GPKQETVYDF WRMVWQENCF SIVMITKLVE VGRVKCCKYW 1000
PDESEMYGDI KITLLKTETL AEYTVRTFAL ERRGYSAKHE VCQFHFTSWP 1050
EHGVPYHATG LLAFIRRVKT STPLDAGPVV VHCSVGAGRT GCYIVLDVML 1100
DMAECEGVVD IYNCVKTLCS RRINMIQTEE QYIFIHDAIL EACLCGETAI 1150
PVNEFALAYK EMLRVDSQSN SSQLREEFQT LNSVTPHLDV EECSIALLPR 1200
NREKNRSMDV LPPDRALAFL VTTEGESNNY INAALMDSFH RPAAFIVTPH 1250
PLPGTTSDFW RLVFDYGCTS VVMLNQLNQS NSAWPCVQYW PEPGLQQYGP 1300
MQVEFLSMSA DEDIITRLFR VKNVTRLQEG QLVVCQFQFL RWSAYRDVPD 1350
SKKAFLNLLA SVQKWQRECG EGRTVVHCLN GGGRSGTYCA SNILMEMIQY 1400
QNIVDVFYAV KTLRNAKPNM VETLEQYRFC YELVLEYLDC LEVR 1444
Length:1,444
Mass (Da):162,661
Last modified:July 22, 2008 - v1
Checksum:iA4C3CDAFFD3D88B1
GO
Isoform 2 (identifier: B3DK56-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     773-782: Missing.

Show »
Length:1,434
Mass (Da):161,302
Checksum:i527A7B61CBA4443A
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei773 – 78210Missing in isoform 2. VSP_037087

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY555586 mRNA. Translation: AAT37515.1.
BC163712 mRNA. Translation: AAI63712.1.
BC163727 mRNA. Translation: AAI63727.1.
RefSeqiNP_001002299.1. NM_001002299.1. [B3DK56-2]
UniGeneiDr.20078.

Genome annotation databases

GeneIDi335096.
KEGGidre:335096.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY555586 mRNA. Translation: AAT37515.1 .
BC163712 mRNA. Translation: AAI63712.1 .
BC163727 mRNA. Translation: AAI63727.1 .
RefSeqi NP_001002299.1. NM_001002299.1. [B3DK56-2 ]
UniGenei Dr.20078.

3D structure databases

ProteinModelPortali B3DK56.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 335096.
KEGGi dre:335096.

Organism-specific databases

CTDi 335096.
ZFINi ZDB-GENE-030131-7036. ptprub.

Phylogenomic databases

eggNOGi NOG311686.
HOGENOMi HOG000049029.
InParanoidi Q6E5N7.
KOi K16662.
PhylomeDBi B3DK56.

Miscellaneous databases

NextBioi 20810666.

Family and domain databases

Gene3Di 2.60.40.10. 4 hits.
3.90.190.10. 2 hits.
InterProi IPR008985. ConA-like_lec_gl_sf.
IPR003961. Fibronectin_type3.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR000998. MAM_dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
[Graphical view ]
Pfami PF00041. fn3. 1 hit.
PF00629. MAM. 1 hit.
PF00102. Y_phosphatase. 2 hits.
[Graphical view ]
PRINTSi PR00020. MAMDOMAIN.
PR00700. PRTYPHPHTASE.
SMARTi SM00060. FN3. 3 hits.
SM00409. IG. 1 hit.
SM00137. MAM. 1 hit.
SM00194. PTPc. 2 hits.
[Graphical view ]
SUPFAMi SSF49265. SSF49265. 2 hits.
SSF49899. SSF49899. 1 hit.
SSF52799. SSF52799. 2 hits.
PROSITEi PS50853. FN3. 3 hits.
PS00740. MAM_1. 1 hit.
PS50060. MAM_2. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 2 hits.
PS50056. TYR_PHOSPHATASE_2. 2 hits.
PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Receptor tyrosine phosphatase psi is required for delta/notch signalling and cyclic gene expression in the presomitic mesoderm."
    Aerne B., Ish-Horowicz D.
    Development 131:3391-3399(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, DEVELOPMENTAL STAGE.
    Tissue: Embryo.
  2. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).

Entry informationi

Entry nameiPTPRU_DANRE
AccessioniPrimary (citable) accession number: B3DK56
Secondary accession number(s): Q6E5N7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: July 22, 2008
Last modified: June 11, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Embryos lacking ptpru display severe disruption of their segmental pattern and loss of cyclic gene expression in the presomitic mesoderm. Convergent extension during gastrulation is also affected.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi