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B3DK56

- PTPRU_DANRE

UniProt

B3DK56 - PTPRU_DANRE

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Protein

Receptor-type tyrosine-protein phosphatase U

Gene

ptpru

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Tyrosine-protein phosphatase which dephosphorylates CTNNB1. May function in cell proliferation and migration and play a role in the maintenance of epithelial integrity. Functions in somitogenesis. Functions as a regulator of the biochemical clock responsible for the segmentation of the presomitic mesoderm.1 Publication

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei1051 – 10511SubstrateSequence Analysis
Active sitei1083 – 10831Phosphocysteine intermediateBy similarity
Binding sitei1127 – 11271SubstrateBy similarity
Active sitei1378 – 13781Phosphocysteine intermediateBy similarity

GO - Molecular functioni

  1. protein tyrosine phosphatase activity Source: UniProtKB-EC

GO - Biological processi

  1. cell adhesion Source: UniProtKB-KW
  2. cell differentiation Source: UniProtKB-KW
  3. somite specification Source: ZFIN
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase, Protein phosphatase, Receptor

Keywords - Biological processi

Cell adhesion, Differentiation

Names & Taxonomyi

Protein namesi
Recommended name:
Receptor-type tyrosine-protein phosphatase U (EC:3.1.3.48)
Short name:
R-PTP-U
Alternative name(s):
Receptor-type protein-tyrosine phosphatase psi
Short name:
R-PTP-psi
Gene namesi
Name:ptpru
Synonyms:rptppsi
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437: Unplaced

Organism-specific databases

ZFINiZDB-GENE-030131-7036. ptprub.

Subcellular locationi

Cell junction By similarity. Cell membrane By similarity; Single-pass type I membrane protein By similarity

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. integral component of membrane Source: ZFIN
  3. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616Sequence AnalysisAdd
BLAST
Chaini17 – 14441428Receptor-type tyrosine-protein phosphatase UPRO_0000371661Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi73 – 731N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi206 ↔ 260Sequence Analysis
Glycosylationi406 – 4061N-linked (GlcNAc...)Sequence Analysis
Glycosylationi684 – 6841N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Developmental stagei

First detected in somites, pronephric duct, midbrain hindbrain boundary, otic vesicle and retina 10-24 after fertilization. When somite formation is complete expression becomes restricted to the retina, the forebrain midbrain, the midbrain hindbrain boundary, the otic vesicle and the branchial arches.1 Publication

Structurei

3D structure databases

ProteinModelPortaliB3DK56.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini17 – 748732ExtracellularSequence AnalysisAdd
BLAST
Topological domaini770 – 1444675CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei749 – 76921HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 184161MAMPROSITE-ProRule annotationAdd
BLAST
Domaini186 – 27186Ig-like C2-typeAdd
BLAST
Domaini284 – 37996Fibronectin type-III 1PROSITE-ProRule annotationAdd
BLAST
Domaini382 – 483102Fibronectin type-III 2PROSITE-ProRule annotationAdd
BLAST
Domaini489 – 590102Fibronectin type-III 3PROSITE-ProRule annotationAdd
BLAST
Domaini597 – 67781Fibronectin type-III 4PROSITE-ProRule annotationAdd
BLAST
Domaini886 – 1142257Tyrosine-protein phosphatase 1PROSITE-ProRule annotationAdd
BLAST
Domaini1174 – 1437264Tyrosine-protein phosphatase 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1083 – 10897Substrate bindingBy similarity

Sequence similaritiesi

Contains 4 fibronectin type-III domains.PROSITE-ProRule annotation
Contains 1 MAM domain.PROSITE-ProRule annotation
Contains 2 tyrosine-protein phosphatase domains.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG311686.
HOGENOMiHOG000049029.
InParanoidiB3DK56.
KOiK16662.
PhylomeDBiB3DK56.

Family and domain databases

Gene3Di2.60.40.10. 4 hits.
3.90.190.10. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR003961. Fibronectin_type3.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR000998. MAM_dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
[Graphical view]
PfamiPF00041. fn3. 1 hit.
PF00629. MAM. 1 hit.
PF00102. Y_phosphatase. 2 hits.
[Graphical view]
PRINTSiPR00020. MAMDOMAIN.
PR00700. PRTYPHPHTASE.
SMARTiSM00060. FN3. 3 hits.
SM00409. IG. 1 hit.
SM00137. MAM. 1 hit.
SM00194. PTPc. 2 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 2 hits.
SSF49899. SSF49899. 1 hit.
SSF52799. SSF52799. 2 hits.
PROSITEiPS50853. FN3. 3 hits.
PS00740. MAM_1. 1 hit.
PS50060. MAM_2. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 2 hits.
PS50056. TYR_PHOSPHATASE_2. 2 hits.
PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: B3DK56-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNTCAFLLIL AVQIHADSVE GPTAGCTFDE DSDPSLCEFS QGEEDDFDWQ
60 70 80 90 100
LFRAHASPHS TSDLLRGSYM MVNSSQHAAG QRAQLLLQTL SENDTHCVQF
110 120 130 140 150
SYFLYSRDGH SPGALRVYVR VNGGPLGIPV WNVSGSRGRQ WHQVELAVST
160 170 180 190 200
FWPNEYQILL EATVDRKGYI AVDDILLLNY PCYKAPHFSR LGDVEVNAGQ
210 220 230 240 250
NATFQCVAAG RPSEAEKFLL ERHNGEVSSG GSVKHLGRNR FAVSFQLEDV
260 270 280 290 300
QKPEQDLYRC VTQSSRGSGV SNFAELIVKV PPSPIAPPQL LRAGSTYLII
310 320 330 340 350
QLNTNSILGD GPIIRREIEY RASLAPWSEI LGVNMVTYKL WHLDPDTEYH
360 370 380 390 400
ISVLLTRPGE GGTGPPGPPL ISRTKCAEPM RALRGLRASE IQSRQLTLQW
410 420 430 440 450
EVPAFNLTRC HTYSVSLCYR YTTAGGGGGH NTTVRECLAV EHNTSRFTLR
460 470 480 490 500
DLPPFHTIQI RLALANTEGK KEGKEVMFQT EEDIPGGIAP ESLTFTPLED
510 520 530 540 550
MIFLKWEEPV EPNGLITQYE ISYQSIESSD PGINVPGPRR TVSKLKNETY
560 570 580 590 600
HMFSNLHPGT TYLISVRART AKGFGQTALT EITTNISAPT FDYGDMPSPL
610 620 630 640 650
SETENTITVL LRPAQGRGAP VSTYQVVVEE EAGRKVKREL GIQDCFPIPT
660 670 680 690 700
SHGEAQARGA PHYYTAELPP SSLSEATPFT VGDNHTYNGY WNSPLDPRKN
710 720 730 740 750
YLVYFQAMSN FRGETRINCI RIARKAACKD HQRALEVTQR SEEMGLILGV
760 770 780 790 800
CAGGLVVLIL LLGAIIIIIK KGRDYYSYSY YPRKPGNMNK TPITYRQEKS
810 820 830 840 850
NMMGSMERSF TDQSTLQEDE RMALSFMDTH TCSTRSDPRS SMNESSSLLG
860 870 880 890 900
GSPRRQCGRK GSPYHTGQLH PAVRVADLLQ HINQMKTAEG YGFKQEYESF
910 920 930 940 950
FDGWDINKKK DKTKGRHDTL MGYDRHRVKL HPLLGDPNSD YINANYIDGY
960 970 980 990 1000
HRSNHFIATQ GPKQETVYDF WRMVWQENCF SIVMITKLVE VGRVKCCKYW
1010 1020 1030 1040 1050
PDESEMYGDI KITLLKTETL AEYTVRTFAL ERRGYSAKHE VCQFHFTSWP
1060 1070 1080 1090 1100
EHGVPYHATG LLAFIRRVKT STPLDAGPVV VHCSVGAGRT GCYIVLDVML
1110 1120 1130 1140 1150
DMAECEGVVD IYNCVKTLCS RRINMIQTEE QYIFIHDAIL EACLCGETAI
1160 1170 1180 1190 1200
PVNEFALAYK EMLRVDSQSN SSQLREEFQT LNSVTPHLDV EECSIALLPR
1210 1220 1230 1240 1250
NREKNRSMDV LPPDRALAFL VTTEGESNNY INAALMDSFH RPAAFIVTPH
1260 1270 1280 1290 1300
PLPGTTSDFW RLVFDYGCTS VVMLNQLNQS NSAWPCVQYW PEPGLQQYGP
1310 1320 1330 1340 1350
MQVEFLSMSA DEDIITRLFR VKNVTRLQEG QLVVCQFQFL RWSAYRDVPD
1360 1370 1380 1390 1400
SKKAFLNLLA SVQKWQRECG EGRTVVHCLN GGGRSGTYCA SNILMEMIQY
1410 1420 1430 1440
QNIVDVFYAV KTLRNAKPNM VETLEQYRFC YELVLEYLDC LEVR
Length:1,444
Mass (Da):162,661
Last modified:July 22, 2008 - v1
Checksum:iA4C3CDAFFD3D88B1
GO
Isoform 2 (identifier: B3DK56-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     773-782: Missing.

Show »
Length:1,434
Mass (Da):161,302
Checksum:i527A7B61CBA4443A
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei773 – 78210Missing in isoform 2. 2 PublicationsVSP_037087

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY555586 mRNA. Translation: AAT37515.1.
BC163712 mRNA. Translation: AAI63712.1.
BC163727 mRNA. Translation: AAI63727.1.
RefSeqiNP_001002299.1. NM_001002299.1. [B3DK56-2]
UniGeneiDr.20078.

Genome annotation databases

GeneIDi335096.
KEGGidre:335096.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY555586 mRNA. Translation: AAT37515.1 .
BC163712 mRNA. Translation: AAI63712.1 .
BC163727 mRNA. Translation: AAI63727.1 .
RefSeqi NP_001002299.1. NM_001002299.1. [B3DK56-2 ]
UniGenei Dr.20078.

3D structure databases

ProteinModelPortali B3DK56.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 335096.
KEGGi dre:335096.

Organism-specific databases

CTDi 335096.
ZFINi ZDB-GENE-030131-7036. ptprub.

Phylogenomic databases

eggNOGi NOG311686.
HOGENOMi HOG000049029.
InParanoidi B3DK56.
KOi K16662.
PhylomeDBi B3DK56.

Miscellaneous databases

NextBioi 20810666.

Family and domain databases

Gene3Di 2.60.40.10. 4 hits.
3.90.190.10. 2 hits.
InterProi IPR013320. ConA-like_dom.
IPR003961. Fibronectin_type3.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR000998. MAM_dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
[Graphical view ]
Pfami PF00041. fn3. 1 hit.
PF00629. MAM. 1 hit.
PF00102. Y_phosphatase. 2 hits.
[Graphical view ]
PRINTSi PR00020. MAMDOMAIN.
PR00700. PRTYPHPHTASE.
SMARTi SM00060. FN3. 3 hits.
SM00409. IG. 1 hit.
SM00137. MAM. 1 hit.
SM00194. PTPc. 2 hits.
[Graphical view ]
SUPFAMi SSF49265. SSF49265. 2 hits.
SSF49899. SSF49899. 1 hit.
SSF52799. SSF52799. 2 hits.
PROSITEi PS50853. FN3. 3 hits.
PS00740. MAM_1. 1 hit.
PS50060. MAM_2. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 2 hits.
PS50056. TYR_PHOSPHATASE_2. 2 hits.
PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Receptor tyrosine phosphatase psi is required for delta/notch signalling and cyclic gene expression in the presomitic mesoderm."
    Aerne B., Ish-Horowicz D.
    Development 131:3391-3399(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, DEVELOPMENTAL STAGE.
    Tissue: Embryo.
  2. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).

Entry informationi

Entry nameiPTPRU_DANRE
AccessioniPrimary (citable) accession number: B3DK56
Secondary accession number(s): Q6E5N7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: July 22, 2008
Last modified: October 29, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Embryos lacking ptpru display severe disruption of their segmental pattern and loss of cyclic gene expression in the presomitic mesoderm. Convergent extension during gastrulation is also affected.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3