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Protein

Receptor-type tyrosine-protein phosphatase U

Gene

ptpru

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Tyrosine-protein phosphatase which dephosphorylates CTNNB1. May function in cell proliferation and migration and play a role in the maintenance of epithelial integrity. Functions in somitogenesis. Functions as a regulator of the biochemical clock responsible for the segmentation of the presomitic mesoderm.1 Publication

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei1051SubstrateSequence analysis1
Active sitei1083Phosphocysteine intermediateBy similarity1
Binding sitei1127SubstrateBy similarity1
Active sitei1378Phosphocysteine intermediateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cell adhesion Source: UniProtKB-KW
  • cell differentiation Source: UniProtKB-KW
  • somite specification Source: ZFIN
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase, Protein phosphatase, Receptor

Keywords - Biological processi

Cell adhesion, Differentiation

Names & Taxonomyi

Protein namesi
Recommended name:
Receptor-type tyrosine-protein phosphatase U (EC:3.1.3.48)
Short name:
R-PTP-U
Alternative name(s):
Receptor-type protein-tyrosine phosphatase psi
Short name:
R-PTP-psi
Gene namesi
Name:ptpru
Synonyms:rptppsi
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-030131-7036. ptprub.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini17 – 748ExtracellularSequence analysisAdd BLAST732
Transmembranei749 – 769HelicalSequence analysisAdd BLAST21
Topological domaini770 – 1444CytoplasmicSequence analysisAdd BLAST675

GO - Cellular componenti

  • cell junction Source: UniProtKB-SubCell
  • integral component of membrane Source: ZFIN
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16Sequence analysisAdd BLAST16
ChainiPRO_000037166117 – 1444Receptor-type tyrosine-protein phosphatase UAdd BLAST1428

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi73N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi206 ↔ 260Sequence analysis
Glycosylationi406N-linked (GlcNAc...)Sequence analysis1
Glycosylationi684N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiB3DK56.

Expressioni

Developmental stagei

First detected in somites, pronephric duct, midbrain hindbrain boundary, otic vesicle and retina 10-24 after fertilization. When somite formation is complete expression becomes restricted to the retina, the forebrain midbrain, the midbrain hindbrain boundary, the otic vesicle and the branchial arches.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000073427.

Structurei

3D structure databases

ProteinModelPortaliB3DK56.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 184MAMPROSITE-ProRule annotationAdd BLAST161
Domaini186 – 271Ig-like C2-typeAdd BLAST86
Domaini284 – 379Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST96
Domaini382 – 483Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST102
Domaini489 – 590Fibronectin type-III 3PROSITE-ProRule annotationAdd BLAST102
Domaini597 – 677Fibronectin type-III 4PROSITE-ProRule annotationAdd BLAST81
Domaini886 – 1142Tyrosine-protein phosphatase 1PROSITE-ProRule annotationAdd BLAST257
Domaini1174 – 1437Tyrosine-protein phosphatase 2PROSITE-ProRule annotationAdd BLAST264

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1083 – 1089Substrate bindingBy similarity7

Sequence similaritiesi

Contains 4 fibronectin type-III domains.PROSITE-ProRule annotation
Contains 1 MAM domain.PROSITE-ProRule annotation
Contains 2 tyrosine-protein phosphatase domains.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4228. Eukaryota.
COG5599. LUCA.
HOGENOMiHOG000049029.
InParanoidiB3DK56.
PhylomeDBiB3DK56.

Family and domain databases

CDDicd00063. FN3. 2 hits.
cd06263. MAM. 1 hit.
Gene3Di2.60.40.10. 4 hits.
3.90.190.10. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR000998. MAM_dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000242. PTPase_domain.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
IPR000387. TYR_PHOSPHATASE_dom.
[Graphical view]
PfamiPF00041. fn3. 1 hit.
PF00629. MAM. 1 hit.
PF00102. Y_phosphatase. 2 hits.
[Graphical view]
PRINTSiPR00020. MAMDOMAIN.
PR00700. PRTYPHPHTASE.
SMARTiSM00060. FN3. 3 hits.
SM00409. IG. 1 hit.
SM00137. MAM. 1 hit.
SM00194. PTPc. 2 hits.
SM00404. PTPc_motif. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF49265. SSF49265. 2 hits.
SSF49899. SSF49899. 1 hit.
SSF52799. SSF52799. 2 hits.
PROSITEiPS50853. FN3. 3 hits.
PS00740. MAM_1. 1 hit.
PS50060. MAM_2. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 2 hits.
PS50056. TYR_PHOSPHATASE_2. 2 hits.
PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: B3DK56-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNTCAFLLIL AVQIHADSVE GPTAGCTFDE DSDPSLCEFS QGEEDDFDWQ
60 70 80 90 100
LFRAHASPHS TSDLLRGSYM MVNSSQHAAG QRAQLLLQTL SENDTHCVQF
110 120 130 140 150
SYFLYSRDGH SPGALRVYVR VNGGPLGIPV WNVSGSRGRQ WHQVELAVST
160 170 180 190 200
FWPNEYQILL EATVDRKGYI AVDDILLLNY PCYKAPHFSR LGDVEVNAGQ
210 220 230 240 250
NATFQCVAAG RPSEAEKFLL ERHNGEVSSG GSVKHLGRNR FAVSFQLEDV
260 270 280 290 300
QKPEQDLYRC VTQSSRGSGV SNFAELIVKV PPSPIAPPQL LRAGSTYLII
310 320 330 340 350
QLNTNSILGD GPIIRREIEY RASLAPWSEI LGVNMVTYKL WHLDPDTEYH
360 370 380 390 400
ISVLLTRPGE GGTGPPGPPL ISRTKCAEPM RALRGLRASE IQSRQLTLQW
410 420 430 440 450
EVPAFNLTRC HTYSVSLCYR YTTAGGGGGH NTTVRECLAV EHNTSRFTLR
460 470 480 490 500
DLPPFHTIQI RLALANTEGK KEGKEVMFQT EEDIPGGIAP ESLTFTPLED
510 520 530 540 550
MIFLKWEEPV EPNGLITQYE ISYQSIESSD PGINVPGPRR TVSKLKNETY
560 570 580 590 600
HMFSNLHPGT TYLISVRART AKGFGQTALT EITTNISAPT FDYGDMPSPL
610 620 630 640 650
SETENTITVL LRPAQGRGAP VSTYQVVVEE EAGRKVKREL GIQDCFPIPT
660 670 680 690 700
SHGEAQARGA PHYYTAELPP SSLSEATPFT VGDNHTYNGY WNSPLDPRKN
710 720 730 740 750
YLVYFQAMSN FRGETRINCI RIARKAACKD HQRALEVTQR SEEMGLILGV
760 770 780 790 800
CAGGLVVLIL LLGAIIIIIK KGRDYYSYSY YPRKPGNMNK TPITYRQEKS
810 820 830 840 850
NMMGSMERSF TDQSTLQEDE RMALSFMDTH TCSTRSDPRS SMNESSSLLG
860 870 880 890 900
GSPRRQCGRK GSPYHTGQLH PAVRVADLLQ HINQMKTAEG YGFKQEYESF
910 920 930 940 950
FDGWDINKKK DKTKGRHDTL MGYDRHRVKL HPLLGDPNSD YINANYIDGY
960 970 980 990 1000
HRSNHFIATQ GPKQETVYDF WRMVWQENCF SIVMITKLVE VGRVKCCKYW
1010 1020 1030 1040 1050
PDESEMYGDI KITLLKTETL AEYTVRTFAL ERRGYSAKHE VCQFHFTSWP
1060 1070 1080 1090 1100
EHGVPYHATG LLAFIRRVKT STPLDAGPVV VHCSVGAGRT GCYIVLDVML
1110 1120 1130 1140 1150
DMAECEGVVD IYNCVKTLCS RRINMIQTEE QYIFIHDAIL EACLCGETAI
1160 1170 1180 1190 1200
PVNEFALAYK EMLRVDSQSN SSQLREEFQT LNSVTPHLDV EECSIALLPR
1210 1220 1230 1240 1250
NREKNRSMDV LPPDRALAFL VTTEGESNNY INAALMDSFH RPAAFIVTPH
1260 1270 1280 1290 1300
PLPGTTSDFW RLVFDYGCTS VVMLNQLNQS NSAWPCVQYW PEPGLQQYGP
1310 1320 1330 1340 1350
MQVEFLSMSA DEDIITRLFR VKNVTRLQEG QLVVCQFQFL RWSAYRDVPD
1360 1370 1380 1390 1400
SKKAFLNLLA SVQKWQRECG EGRTVVHCLN GGGRSGTYCA SNILMEMIQY
1410 1420 1430 1440
QNIVDVFYAV KTLRNAKPNM VETLEQYRFC YELVLEYLDC LEVR
Length:1,444
Mass (Da):162,661
Last modified:July 22, 2008 - v1
Checksum:iA4C3CDAFFD3D88B1
GO
Isoform 2 (identifier: B3DK56-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     773-782: Missing.

Show »
Length:1,434
Mass (Da):161,302
Checksum:i527A7B61CBA4443A
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_037087773 – 782Missing in isoform 2. 2 Publications10

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY555586 mRNA. Translation: AAT37515.1.
BC163712 mRNA. Translation: AAI63712.1.
BC163727 mRNA. Translation: AAI63727.1.
UniGeneiDr.20078.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY555586 mRNA. Translation: AAT37515.1.
BC163712 mRNA. Translation: AAI63712.1.
BC163727 mRNA. Translation: AAI63727.1.
UniGeneiDr.20078.

3D structure databases

ProteinModelPortaliB3DK56.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000073427.

Proteomic databases

PaxDbiB3DK56.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

ZFINiZDB-GENE-030131-7036. ptprub.

Phylogenomic databases

eggNOGiKOG4228. Eukaryota.
COG5599. LUCA.
HOGENOMiHOG000049029.
InParanoidiB3DK56.
PhylomeDBiB3DK56.

Miscellaneous databases

PROiB3DK56.

Family and domain databases

CDDicd00063. FN3. 2 hits.
cd06263. MAM. 1 hit.
Gene3Di2.60.40.10. 4 hits.
3.90.190.10. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR000998. MAM_dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000242. PTPase_domain.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
IPR000387. TYR_PHOSPHATASE_dom.
[Graphical view]
PfamiPF00041. fn3. 1 hit.
PF00629. MAM. 1 hit.
PF00102. Y_phosphatase. 2 hits.
[Graphical view]
PRINTSiPR00020. MAMDOMAIN.
PR00700. PRTYPHPHTASE.
SMARTiSM00060. FN3. 3 hits.
SM00409. IG. 1 hit.
SM00137. MAM. 1 hit.
SM00194. PTPc. 2 hits.
SM00404. PTPc_motif. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF49265. SSF49265. 2 hits.
SSF49899. SSF49899. 1 hit.
SSF52799. SSF52799. 2 hits.
PROSITEiPS50853. FN3. 3 hits.
PS00740. MAM_1. 1 hit.
PS50060. MAM_2. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 2 hits.
PS50056. TYR_PHOSPHATASE_2. 2 hits.
PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTPRU_DANRE
AccessioniPrimary (citable) accession number: B3DK56
Secondary accession number(s): Q6E5N7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: July 22, 2008
Last modified: November 30, 2016
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Embryos lacking ptpru display severe disruption of their segmental pattern and loss of cyclic gene expression in the presomitic mesoderm. Convergent extension during gastrulation is also affected.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.