B3DJM5 (TSH1_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Teashirt homolog 1

Alternative name(s):
Teashirt-like zinc finger protein

Gene names

Name:	tshz1
Synonyms:	sdccag33, tsrt

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	1158 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Probable transcriptional regulator involved in developmental processes. May act as a transcriptional repressor Potential.
Subcellular location	Nucleus By similarity.
Developmental stage	Expressed in the 2-somite stage (10 hpf) in the neural keel between rhombomere 7 (r7) and the anterior spinal cord. Between the 2- and 20-somite stages (10-19 hpf), expressed caudally in the developing spinal cord. At 25 somite stage (21 hpf), detected throughout the spinal cord with expression levels gradually decreasing from anterior to posterior. At the prim-5 stage (24 hpf), detected in the pectoral fin buds and in the dorsal forebrain. At the long-pec stage (48 hpf), expressed in the telecephalon, tectum opticum, hindbrain, cerebellum, in the first pharyngeal arch, in the eyes and at very low level in the olfactory placodes. Around the protruding mouth stage (72-96 hpf), some expression is still detected in the eyes and only scattered expression is seen in the tectum, midbrain/hindbrain boundary and hindbrain. By the early larval stage (120 hpf), no expression is detected in the first pharyngeal arch and only low expression is detectable in the telecephalon, midbrain/hindbrain boundary, hindbrain and along the edges of the tectum. Ref.2
Induction	Up-regulated in embryos by all-trans retinoic acid at the prim-5 stage (24 hpf) in more anterior regions of the neural tube, especially in the midbrain, with weaker expression in the forebrain and in the eye primordium. Ref.2
Sequence similarities	Belongs to the teashirt C2H2-type zinc-finger protein family. Contains 5 C2H2-type zinc fingers. Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Homeobox Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Developmental protein Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1158	1158	Teashirt homolog 1		PRO_0000399473
Regions
	Zinc finger	288 – 312	25	C2H2-type 1
	Zinc finger	349 – 373	25	C2H2-type 2
	Zinc finger	461 – 485	25	C2H2-type 3
	DNA binding	963 – 1033	71	Homeobox
	Zinc finger	1048 – 1070	23	C2H2-type 4
	Zinc finger	1115 – 1138	24	C2H2-type 5
	Compositional bias	148 – 216	69	Ser-rich

Sequences

	Sequence		Length	Mass (Da)
	B3DJM5 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: EEBE63EC8A9EF62B	FASTA	1,158	127,921
	10 20 30 40 50 60 MISLILQFML KLHILGTILS HHLFFPLCFV FSAYVPEDEL KAAKIDEEHL QDDGLSLDGQ 70 80 90 100 110 120 DAEYLCNEED DGREQLSYQN SPLSNGTNPD AGYGSPLSDT SDHLADFKST SSKEGQDKEE 130 140 150 160 170 180 VEDMDTDAKL SLQDSLAQMK AVYANLISDA SWSSITKDIM KSKQVSASSI NSTSSSHKGN 190 200 210 220 230 240 NSVANSHAST IASSGASSSS NASASTKTNV TQSSNSTKAT TLTNANNGTI NGANSGGVAY 250 260 270 280 290 300 DWHQAALAKT LQHTPYHLMP EPSLFSTVQL YRQNNKLYGP VFTGASKFRC KDCSAAYDTL 310 320 330 340 350 360 VGLTVHMNET GHYRDDNKDK EEDRGKKWSK PRKRSLMEME GKEDAQKVLK CMYCGHSFES 370 380 390 400 410 420 LQDLSVHMIK TKHYQKVPLK EPMPALASKL VPSTKKRVFQ DLMSPCSPDS ISSTPGIPLA 430 440 450 460 470 480 ETAPTKDPKI SNPYVTANNR YGYQNGASYT WQFEARKAQI LKCMECGSSH DTLQQLTAHM 490 500 510 520 530 540 MVTGHFLKVT NSASKKGKQL VFDPVVEEKI QSIPLPPTTT RLPAPAIKSQ PDSPIHPSIM 550 560 570 580 590 600 DDRKELEEEK FEEPEEKKIK QEKEDPSERV EKSEKLSHYK YLREEDLEES PKGGLDILKS 610 620 630 640 650 660 LENTVSSAIS KAQTGTPTWG GYPSIHAAYQ LQGSVKSSIP AIQSVQIQPT FNASSLKSLT 670 680 690 700 710 720 SDSSTLIHSP SSPSPPPNHK SNVLAMEELV EKVTGKIPSK KDRDEKLTER NSKHLTAELP 730 740 750 760 770 780 SPVLKERKDL PKPDDLTKPT KNGTVDKDLE HTPVREGEYK ESHADNPIKN GTDALKTQVS 790 800 810 820 830 840 NGCGNLGIIT DHSPEQPLVN PLSALQSIMN THLGKASKTV SPLLDPLAML YKISNNMMEK 850 860 870 880 890 900 PMYNPAQVKQ VEPINRYYEN DDDQPMDLTK SKSGIGPTNN CTSTIISNSS ITNSTRPILS 910 920 930 940 950 960 TLAEQVSSPL RENALMDISD MVKNLTGRLT PKSSTPSSIS EKSDADGCAF EDGLEDLSPI 970 980 990 1000 1010 1020 QKRKGRQSNW NPQHLLILQA QFASSLRETP DGKYIITDLG PQERVHICKF TGLSMTTISH 1030 1040 1050 1060 1070 1080 WLANVKYQLR RTGGTKFLKN IDSGHPLFLC SDCASQFRTP STYINHLESH LGFSLKDLSK 1090 1100 1110 1120 1130 1140 LSIDLIRDQQ AVTKMITDKT FRALDLNEED SNSIFQCKLC NRTFVSKHAV KLHLSKTHGK 1150 SPEDHLIFVT ELEKLEKA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	"Isolation and expression of zebrafish zinc-finger transcription factor gene tsh1." Wang H., Lee E.M.-J., Sperber S.M., Lin S., Ekker M., Long Q. Gene Expr. Patterns 7:318-322(2007) [PubMed: 17035100] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 307-1158, INDUCTION, DEVELOPMENTAL STAGE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF242292 mRNA. Translation: AAL88747.1. BC163536 mRNA. Translation: AAI63536.1.
IPI	IPI00485496.
RefSeq	NP_001036211.1. NM_001042746.1.
UniGene	Dr.16111.
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	B3DJM5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000024639; ENSDARP00000023365; ENSDARG00000005026.
GeneID	369196.
KEGG	dre:369196.
Organism-specific databases
CTD	10194.
ZFIN	ZDB-GENE-030820-3. tshz1.
Phylogenomic databases
HOGENOM	HBG445553.
HOVERGEN	HBG079626.
InParanoid	B3DJM5.
OMA	KKRALQD.
OrthoDB	EOG49S65M.
Gene expression databases
Bgee	B3DJM5.
Family and domain databases
InterPro	IPR001356. Homeobox. IPR009057. Homeodomain-like. IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. [Graphical view]
KO	K09236.
Pfam	PF00096. zf-C2H2. 1 hit. [Graphical view]
SMART	SM00389. HOX. 1 hit. SM00355. ZnF_C2H2. 5 hits. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 1 hit.
PROSITE	PS00027. HOMEOBOX_1. False negative. PS50071. HOMEOBOX_2. False negative. PS00028. ZINC_FINGER_C2H2_1. 4 hits. PS50157. ZINC_FINGER_C2H2_2. 3 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TSH1_DANRE

Accession

Primary (citable) accession number: B3DJM5
Secondary accession number(s): Q8QGQ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 5, 2010
Last sequence update:	July 22, 2008
Last modified:	December 14, 2011
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program