B3CTU0 (B3CTU0_ORITI) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Methionyl-tRNA formyltransferase HAMAP-Rule MF_00182
EC=2.1.2.9 HAMAP-Rule MF_00182

Gene names

Name:	fmt HAMAP-Rule MF_00182 EMBL BAG40787.1
Ordered Locus Names:	OTT_1329 EMBL BAG40787.1

Organism

Orientia tsutsugamushi (strain Ikeda) (Rickettsia tsutsugamushi) [Complete proteome] [HAMAP] EMBL BAG40787.1

Taxonomic identifier

334380 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Orientia › Orientia tsutsugamushi

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by: (I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-GTP By similarity. HAMAP-Rule MF_00182 SAAS SAAS005794
Catalytic activity	10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = tetrahydrofolate + N-formylmethionyl-tRNA(fMet). HAMAP-Rule MF_00182 SAAS SAAS005794
Sequence similarities	Belongs to the fmt family. HAMAP-Rule MF_00182

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP-Rule MF_00182 SAAS SAAS005794
Molecular function	Methyltransferase HAMAP-Rule MF_00182 SAAS SAAS005794 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	translational initiation Inferred from electronic annotation. Source: GOC
Molecular_function	methionyl-tRNA formyltransferase activity Inferred from electronic annotation. Source: HAMAP methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	104 – 107	4	Tetrahydrofolate (THF) binding By similarity HAMAP-Rule MF_00182

Sequences

	Sequence		Length	Mass (Da)
	B3CTU0 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: DF7335DB317D8AD8	FASTA	302	33,732
	10 20 30 40 50 60 MGSPEFAIPA LKELALSKHN VIAVFTSKPK KRDRYLNIQR SPIHKLASAL SIPVYTPDSL 70 80 90 100 110 120 KTNDVQNLIA TLDADVIVVA AYGLIIPKAI LKMKKYGCIN IHPSMLPKYR GAAPIQRTII 130 140 150 160 170 180 NGEKETAVCI IQMDQGVDTG DIILCQKFHL AKNICFSELH DQCAKVGAKL LVKAINYIHT 190 200 210 220 230 240 LPRIPQSQDR ASYAHKLSKS ESKINWYESA YTIDCKIRGM NPWPGTFFTY NNCNIKVLKA 250 260 270 280 290 300 KIVNNSHNLQ PGTVKIVNNN KLLVACQEHF LELLSLQLPG RKELSSSQFL CGYHILPDTV LQ

References

[1]

"The whole-genome sequencing of the obligate intracellular bacterium Orientia tsutsugamushi revealed massive gene amplification during reductive genome evolution." Nakayama K., Yamashita A., Kurokawa K., Morimoto T., Ogawa M., Fukuhara M., Urakami H., Ohnishi M., Uchiyama I., Ogura Y., Ooka T., Oshima K., Tamura A., Hattori M., Hayashi T. DNA Res. 15:185-199(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ikeda.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008981 Genomic DNA. Translation: BAG40787.1.
RefSeq	YP_001938021.1. NC_010793.1.
3D structure databases
ProteinModelPortal	B3CTU0.
ModBase	Search...
Protein-protein interaction databases
STRING	334380.OTT_1329.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAG40787; BAG40787; OTT_1329.
GeneID	6337195.
KEGG	ott:OTT_1329.
PATRIC	22828962. VBIOriTsu129072_1495.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0223.
HOGENOM	HOG000261177.
KO	K00604.
OMA	DWNKSAR.
ProtClustDB	PRK00005.
Enzyme and pathway databases
BioCyc	OTSU334380:GC7O-1355-MONOMER.
Family and domain databases
Gene3D	3.10.25.10. 1 hit. 3.40.50.170. 1 hit.
HAMAP	MF_00182. Formyl_trans.
InterPro	IPR005794. Fmt. IPR005793. Formyl_trans_C. IPR002376. Formyl_transf_N. IPR011034. Formyl_transferase_C-like. IPR015518. Met_tRNA_Form_TA-like. [Graphical view]
PANTHER	PTHR11138. PTHR11138. 1 hit.
Pfam	PF02911. Formyl_trans_C. 1 hit. PF00551. Formyl_trans_N. 1 hit. [Graphical view]
SUPFAM	SSF50486. FMT_C_like. 1 hit. SSF53328. formyl_transf. 1 hit.
TIGRFAMs	TIGR00460. fmt. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B3CTU0_ORITI

Accession

Primary (citable) accession number: B3CTU0

Entry history

Integrated into UniProtKB/TrEMBL:	July 22, 2008
Last sequence update:	July 22, 2008
Last modified:	May 29, 2013
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)