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B3CST4

- SYE2_ORITI

UniProt

B3CST4 - SYE2_ORITI

Protein

Glutamate--tRNA ligase 2

Gene

gltX2

Organism
Orientia tsutsugamushi (strain Ikeda) (Rickettsia tsutsugamushi)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 39 (01 Oct 2014)
      Sequence version 2 (24 Mar 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

    Catalytic activityi

    ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei245 – 2451ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
    3. tRNA binding Source: InterPro

    GO - Biological processi

    1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciOTSU334380:GC7O-991-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate--tRNA ligase 2UniRule annotation (EC:6.1.1.17UniRule annotation)
    Alternative name(s):
    Glutamyl-tRNA synthetase 2UniRule annotation
    Short name:
    GluRS 2UniRule annotation
    Gene namesi
    Name:gltX2UniRule annotation
    Ordered Locus Names:OTT_0973
    OrganismiOrientia tsutsugamushi (strain Ikeda) (Rickettsia tsutsugamushi)
    Taxonomic identifieri334380 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeOrientia
    ProteomesiUP000001033: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 474474Glutamate--tRNA ligase 2PRO_0000367728Add
    BLAST

    Proteomic databases

    PRIDEiB3CST4.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi334380.OTT_0973.

    Structurei

    3D structure databases

    ProteinModelPortaliB3CST4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi10 – 2011"HIGH" regionAdd
    BLAST
    Motifi242 – 2465"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0008.
    HOGENOMiHOG000252722.
    KOiK01885.
    OrthoDBiEOG6DRPF7.

    Family and domain databases

    Gene3Di1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPiMF_00022_B. Glu_tRNA_synth_B.
    InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR001412. aa-tRNA-synth_I_CS.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR10119. PTHR10119. 1 hit.
    PfamiPF00749. tRNA-synt_1c. 1 hit.
    [Graphical view]
    PRINTSiPR00987. TRNASYNTHGLU.
    SUPFAMiSSF48163. SSF48163. 1 hit.
    TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B3CST4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTNIVTRFAP SPTGFLHIGG ARTALFNYLF ARHHKGKFLL RIEDTDAARS    50
    TEEYKLGIID SLKWLKINWD NDIFYQSANL QRHVNVALEL VKSGKAYYCF 100
    TSQEEIDLQR QLAIAQKQSF IFRSPWRNNI PSSLSLKNNN KAYVIRFKAP 150
    NHGTTIINDY VQGKVVFQNQ QIDDMILVRS DGTPTYMLAV VVDDHDMGIT 200
    HIIRGDDHLT NAAKQIALYD ALGWKAPAMV HIPLIYGPDG TKLSKRHGAI 250
    GVDAYQKMGY LPEALCNYLL RLGWSYQDEE IISHERAIKL FDIDGLGKSA 300
    ARLDFDKMLY LNGYYIRSTD NSILAKLVIE ILSKQYILSN ESCNLIQKGM 350
    SGLKSRANLL TDLAENAKIY VLENQLTFVN EALNIIQKTP SMLITEVIDI 400
    INNLQELNCE SVKQSLTEFA KVKHMKLGQL MDPIRALLTG NTKSPSIFEV 450
    IPILGKIHAV KRLADIEAIK SNNQ 474
    Length:474
    Mass (Da):53,576
    Last modified:March 24, 2009 - v2
    Checksum:i3ACE63C5EB0C601A
    GO

    Sequence cautioni

    The sequence BAG40431.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008981 Genomic DNA. Translation: BAG40431.1. Different initiation.
    RefSeqiYP_001937665.1. NC_010793.1.

    Genome annotation databases

    EnsemblBacteriaiBAG40431; BAG40431; OTT_0973.
    GeneIDi6337729.
    KEGGiott:OTT_0973.
    PATRICi22828164. VBIOriTsu129072_1103.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008981 Genomic DNA. Translation: BAG40431.1 . Different initiation.
    RefSeqi YP_001937665.1. NC_010793.1.

    3D structure databases

    ProteinModelPortali B3CST4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 334380.OTT_0973.

    Proteomic databases

    PRIDEi B3CST4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAG40431 ; BAG40431 ; OTT_0973 .
    GeneIDi 6337729.
    KEGGi ott:OTT_0973.
    PATRICi 22828164. VBIOriTsu129072_1103.

    Phylogenomic databases

    eggNOGi COG0008.
    HOGENOMi HOG000252722.
    KOi K01885.
    OrthoDBi EOG6DRPF7.

    Enzyme and pathway databases

    BioCyci OTSU334380:GC7O-991-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPi MF_00022_B. Glu_tRNA_synth_B.
    InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR001412. aa-tRNA-synth_I_CS.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR10119. PTHR10119. 1 hit.
    Pfami PF00749. tRNA-synt_1c. 1 hit.
    [Graphical view ]
    PRINTSi PR00987. TRNASYNTHGLU.
    SUPFAMi SSF48163. SSF48163. 1 hit.
    TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The whole-genome sequencing of the obligate intracellular bacterium Orientia tsutsugamushi revealed massive gene amplification during reductive genome evolution."
      Nakayama K., Yamashita A., Kurokawa K., Morimoto T., Ogawa M., Fukuhara M., Urakami H., Ohnishi M., Uchiyama I., Ogura Y., Ooka T., Oshima K., Tamura A., Hattori M., Hayashi T.
      DNA Res. 15:185-199(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Ikeda.

    Entry informationi

    Entry nameiSYE2_ORITI
    AccessioniPrimary (citable) accession number: B3CST4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: March 24, 2009
    Last modified: October 1, 2014
    This is version 39 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3