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B3CSI0

- RUVC_ORITI

UniProt

B3CSI0 - RUVC_ORITI

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Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Orientia tsutsugamushi (strain Ikeda) (Rickettsia tsutsugamushi)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg(2+) ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi7 – 71MagnesiumUniRule annotation
Metal bindingi67 – 671MagnesiumUniRule annotation
Metal bindingi139 – 1391MagnesiumUniRule annotation
Metal bindingi142 – 1421MagnesiumUniRule annotation

GO - Molecular functioni

  1. crossover junction endodeoxyribonuclease activity Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. nucleic acid binding Source: InterPro

GO - Biological processi

  1. DNA recombination Source: UniProtKB-HAMAP
  2. DNA repair Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciOTSU334380:GC7O-940-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RuvCUniRule annotation (EC:3.1.22.4UniRule annotation)
Alternative name(s):
Holliday junction nuclease RuvCUniRule annotation
Holliday junction resolvase RuvCUniRule annotation
Gene namesi
Name:ruvCUniRule annotation
Ordered Locus Names:OTT_0923
OrganismiOrientia tsutsugamushi (strain Ikeda) (Rickettsia tsutsugamushi)
Taxonomic identifieri334380 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeOrientia
ProteomesiUP000001033: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 159159Crossover junction endodeoxyribonuclease RuvCPRO_1000090543Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi334380.OTT_0923.

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvC family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0817.
HOGENOMiHOG000012181.
KOiK01159.
OMAiMQIKLAL.
OrthoDBiEOG6RG044.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC.
InterProiIPR012337. RNaseH-like_dom.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamiPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00228. ruvC. 1 hit.

Sequencei

Sequence statusi: Complete.

B3CSI0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIILGIDPSL VSTGWGVISI SGSIVNYIDS GVIKTVSKDS LVLKLGNISL
60 70 80 90 100
MIEKLITRFN PFHVAMEEVF INKNYSSSVT LIQARGAIMS VIGRYNIDFS
110 120 130 140 150
EYAPNKIKKA IVGAGKAEKH QVQQMVKLLM HIKKAISKDE SDALATAYTA

SVNHQIKII
Length:159
Mass (Da):17,391
Last modified:July 22, 2008 - v1
Checksum:iAD860103EAA25C22
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008981 Genomic DNA. Translation: BAG40381.1.
RefSeqiWP_012461508.1. NC_010793.1.
YP_001937615.1. NC_010793.1.

Genome annotation databases

EnsemblBacteriaiBAG40381; BAG40381; OTT_0923.
GeneIDi6336909.
KEGGiott:OTT_0923.
PATRICi22828044. VBIOriTsu129072_1044.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008981 Genomic DNA. Translation: BAG40381.1 .
RefSeqi WP_012461508.1. NC_010793.1.
YP_001937615.1. NC_010793.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 334380.OTT_0923.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAG40381 ; BAG40381 ; OTT_0923 .
GeneIDi 6336909.
KEGGi ott:OTT_0923.
PATRICi 22828044. VBIOriTsu129072_1044.

Phylogenomic databases

eggNOGi COG0817.
HOGENOMi HOG000012181.
KOi K01159.
OMAi MQIKLAL.
OrthoDBi EOG6RG044.

Enzyme and pathway databases

BioCyci OTSU334380:GC7O-940-MONOMER.

Family and domain databases

Gene3Di 3.30.420.10. 1 hit.
HAMAPi MF_00034. RuvC.
InterProi IPR012337. RNaseH-like_dom.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view ]
Pfami PF02075. RuvC. 1 hit.
[Graphical view ]
PRINTSi PR00696. RSOLVASERUVC.
SUPFAMi SSF53098. SSF53098. 1 hit.
TIGRFAMsi TIGR00228. ruvC. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The whole-genome sequencing of the obligate intracellular bacterium Orientia tsutsugamushi revealed massive gene amplification during reductive genome evolution."
    Nakayama K., Yamashita A., Kurokawa K., Morimoto T., Ogawa M., Fukuhara M., Urakami H., Ohnishi M., Uchiyama I., Ogura Y., Ooka T., Oshima K., Tamura A., Hattori M., Hayashi T.
    DNA Res. 15:185-199(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ikeda.

Entry informationi

Entry nameiRUVC_ORITI
AccessioniPrimary (citable) accession number: B3CSI0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 22, 2008
Last modified: November 26, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3