B3CRX8 (SYE1_ORITI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase 1
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase 1
Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	OTT_0777

Organism

Orientia tsutsugamushi (strain Ikeda) (Rickettsia tsutsugamushi) [Complete proteome] [HAMAP]

Taxonomic identifier

334380 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Orientia

Protein attributes

Sequence length	448 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 448	448	Glutamate--tRNA ligase 1 HAMAP MF_00022_B		PRO_0000367727
Regions
	Motif	9 – 19	11	"HIGH" region HAMAP MF_00022_B
	Motif	240 – 244	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	243	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3CRX8 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 6D980FEC9C77418E	FASTA	448	51,379
	10 20 30 40 50 60 MTVITRFAPS PTGKLHIGNV RVALVNWLYA QKYNGNFILR IDDTDRDRSK VEYHEAIIKD 70 80 90 100 110 120 LQWLGINWNS SFLQSTRFNK YAAAKQYLIS SGRLYECYET PEMLEIERKR QLASGYPPIY 130 140 150 160 170 180 SRKSLELTTA QKVQLQAEGY KVHYRFLIDR NKPIIWNDLI KGEIKYDGSN VSDPIVIKED 190 200 210 220 230 240 GTMIYMLCSV IDDIEYRISH IIRGEDHITN TAIQIQMFEA LGAAIPQLGH LSLIKSDSGK 250 260 270 280 290 300 ISKRIGGFTI DYLKDQLGIE PMAVNNLLAL SGTSNNVDAY FSLESLITKF DLSAFSKSTI 310 320 330 340 350 360 IYNENELVTL NHKLLVNTEY DTIKHRLTAI GLPDVTKEFW LAVRHNLNTL NDIKIWWQIC 370 380 390 400 410 420 YLPTLDKFQE QDAEFLKLAA ELLPSGKLTD NSWDDWVQNI IKATNRRGKA LFMPLRLALT 430 440 GITYGPELKY LLPLIGGDEV RARLLRYQ

References

[1]

"The whole-genome sequencing of the obligate intracellular bacterium Orientia tsutsugamushi revealed massive gene amplification during reductive genome evolution." Nakayama K., Yamashita A., Kurokawa K., Morimoto T., Ogawa M., Fukuhara M., Urakami H., Ohnishi M., Uchiyama I., Ogura Y., Ooka T., Oshima K., Tamura A., Hattori M., Hayashi T. DNA Res. 15:185-199(2008) [PubMed: 18508905] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ikeda.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008981 Genomic DNA. Translation: BAG40235.1.
RefSeq	YP_001937469.1. NC_010793.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B3CRX8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6337915.
GenomeReviews	Gene locus OTT_0777 in contig AP008981_GR.
KEGG	ott:OTT_0777.
PATRIC	22827698. VBIOriTsu129072_0879.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
OMA	LLYPCYE.
ProtClustDB	PRK12558.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_ORITI

Accession

Primary (citable) accession number: B3CRX8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 22, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program