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B3CQP5

- LIPA_ORITI

UniProt

B3CQP5 - LIPA_ORITI

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Protein

Lipoyl synthase

Gene
lipA, OTT_0086
Organism
Orientia tsutsugamushi (strain Ikeda) (Rickettsia tsutsugamushi)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi44 – 441Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi49 – 491Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi55 – 551Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi70 – 701Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi74 – 741Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi77 – 771Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciOTSU334380:GC7O-88-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:OTT_0086
OrganismiOrientia tsutsugamushi (strain Ikeda) (Rickettsia tsutsugamushi)
Taxonomic identifieri334380 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeOrientia
ProteomesiUP000001033: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 302302Lipoyl synthaseUniRule annotationPRO_1000099617Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi334380.OTT_0086.

Structurei

3D structure databases

ProteinModelPortaliB3CQP5.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235998.
KOiK03644.
OMAiHPHIPTK.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

B3CQP5-1 [UniParc]FASTAAdd to Basket

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MCSDKNITAT ALVRPSWLRV KAPFSDEYQS TNELIKSLKL NTVCKEAACP    50
NIGECWSKKH ATVMILGSIC TRACAFCNVS TGKPEQVDEY EPYRLSEAVM 100
KLGLKHVVIT SVDRDDISDG GASHFAKCIT YIRERSPSTS IEVLTPDFLR 150
KHDAWKIVAK ARPDVYNHNI ETVPSLYLKV RPGARYYNSL NLLHQVKIFD 200
SSIFTKSGIM VGLGETKHEV LQVMDDLRAA EVDFLTIGQY LRPSARHIDV 250
DRYVAPDEFD YYARVAKSKG FLMVSASPLT RSSYHAGEHF EKLKQMRLQN 300
II 302
Length:302
Mass (Da):34,099
Last modified:July 22, 2008 - v1
Checksum:i9C3EA57A02D1BB7F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP008981 Genomic DNA. Translation: BAG39544.1.
RefSeqiYP_001936778.1. NC_010793.1.

Genome annotation databases

EnsemblBacteriaiBAG39544; BAG39544; OTT_0086.
GeneIDi6338111.
KEGGiott:OTT_0086.
PATRICi22826134. VBIOriTsu129072_0104.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP008981 Genomic DNA. Translation: BAG39544.1 .
RefSeqi YP_001936778.1. NC_010793.1.

3D structure databases

ProteinModelPortali B3CQP5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 334380.OTT_0086.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAG39544 ; BAG39544 ; OTT_0086 .
GeneIDi 6338111.
KEGGi ott:OTT_0086.
PATRICi 22826134. VBIOriTsu129072_0104.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235998.
KOi K03644.
OMAi HPHIPTK.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci OTSU334380:GC7O-88-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The whole-genome sequencing of the obligate intracellular bacterium Orientia tsutsugamushi revealed massive gene amplification during reductive genome evolution."
    Nakayama K., Yamashita A., Kurokawa K., Morimoto T., Ogawa M., Fukuhara M., Urakami H., Ohnishi M., Uchiyama I., Ogura Y., Ooka T., Oshima K., Tamura A., Hattori M., Hayashi T.
    DNA Res. 15:185-199(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ikeda.

Entry informationi

Entry nameiLIPA_ORITI
AccessioniPrimary (citable) accession number: B3CQP5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 22, 2008
Last modified: May 14, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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