B3CQ12 (B3CQ12_ORITI) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Leucine--tRNA ligase HAMAP-Rule MF_00049
EC=6.1.1.4 HAMAP-Rule MF_00049

Alternative name(s):
Leucyl-tRNA synthetase HAMAP-Rule MF_00049

Gene names

Name:	leuS HAMAP-Rule MF_00049
Ordered Locus Names:	OTT_0245

Organism

Orientia tsutsugamushi (strain Ikeda) (Rickettsia tsutsugamushi) [Complete proteome] [HAMAP] EMBL BAG39703.1

Taxonomic identifier

334380 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Orientia › Orientia tsutsugamushi

Protein attributes

Sequence length	839 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00049.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. HAMAP-Rule MF_00049

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP-Rule MF_00049
Cellular component	Cytoplasm HAMAP-Rule MF_00049
Ligand	ATP-binding HAMAP-Rule MF_00049 Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase HAMAP-Rule MF_00049 EMBL BAG39703.1 Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	leucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP regulation of translational fidelity Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro leucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Motif	38 – 48	11	"HIGH" region By similarity HAMAP-Rule MF_00049
	Motif	609 – 613	5	"KMSKS" region By similarity HAMAP-Rule MF_00049
Sites
	Binding site	612	1	ATP By similarity HAMAP-Rule MF_00049

Sequences

	Sequence		Length	Mass (Da)
	B3CQ12 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: FC01E81483EFE1A0	FASTA	839	96,732
	10 20 30 40 50 60 MDLVKIEKKW QEIWEQNEVF KVKEILNKIK RYILPMFPYP SGKAHVGHVR NYTICDVIAR 70 80 90 100 110 120 FERSQGHNVL HAMGWDAFGL PAENAAMQNN THPNSWVNSN VDVMKRQLKA IGLSYDWSRE 130 140 150 160 170 180 IITCSPQYYV HEQRFFLEML KKGLVYQKES LVNWDPVDQT VLANEQVING KGWRSGAIIE 190 200 210 220 230 240 YRNLKQWFIK ITNYADALLK GLDSLQSWPE SVKTMQKKWI GQSKGINIDF QLKGIEASIK 250 260 270 280 290 300 VFSTKPETLF GASFIALSYN HTLVQQYVHT TPEIQEFIDK CSNVGTSNVN IDKMSKLAVL 310 320 330 340 350 360 TNLKVIHPLN SSIELPVILS NFVLMDYGTG ALFGCPAHDE RDHEIAKLLK LNIKQVITST 370 380 390 400 410 420 ERDIDVLKEA YVGDGIMINS FHLNGLTTAE ARQKVINELQ HKNIGQQVTN YKLKDWGISR 430 440 450 460 470 480 QRFWGCPIPI IHCKSCGAVP VPYEDLPVIL PEHGVEFTGK GNPLDNHPSW KYVKCPKCHL 490 500 510 520 530 540 DAVRETDTFD TFFESSWYFA RFCNSTSDDM VDAKAAKYWL PVDQYIGGIE HAVMHLLYAR 550 560 570 580 590 600 FITRVMYDLK YIDIQEPFTS LITQGMVLHR TYQDQSNNWV YPNEVEVDSN GKLRCKADSQ 610 620 630 640 650 660 YVTVGKLEKM SKSKKNVVDL ELVLKLYGAD VARMFVLSDT PPEKDLEWST EGIEGCYKFV 670 680 690 700 710 720 QKLYNFALKL KNINLTDNKI DKVLLNKTHK TIKNVTQDII SCRLNKAIAR LRELYNLIFK 730 740 750 760 770 780 MSELTVQIKE SFLILIRLFN PFIPHLSEEI WSLLSDGEKM LVELPWPKYE EKYIHEEEHI 790 800 810 820 830 TIAIQINGKL RSLYNCLIDT PEHDVQSAIL KLEQVKKHIG DKEVRKCIFI PNKLINIIV

References

[1]

"The whole-genome sequencing of the obligate intracellular bacterium Orientia tsutsugamushi revealed massive gene amplification during reductive genome evolution." Nakayama K., Yamashita A., Kurokawa K., Morimoto T., Ogawa M., Fukuhara M., Urakami H., Ohnishi M., Uchiyama I., Ogura Y., Ooka T., Oshima K., Tamura A., Hattori M., Hayashi T. DNA Res. 15:185-199(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ikeda.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008981 Genomic DNA. Translation: BAG39703.1.
RefSeq	YP_001936937.1. NC_010793.1.
3D structure databases
ProteinModelPortal	B3CQ12.
ModBase	Search...
Protein-protein interaction databases
STRING	334380.OTT_0245.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAG39703; BAG39703; OTT_0245.
GeneID	6336391.
KEGG	ott:OTT_0245.
PATRIC	22826492. VBIOriTsu129072_0281.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0495.
HOGENOM	HOG000200747.
KO	K01869.
OMA	GWPEQVK.
ProtClustDB	PRK00390.
Family and domain databases
Gene3D	3.40.50.620. 3 hits. 3.90.740.10. 1 hit.
HAMAP	MF_00049_B. Leu_tRNA_synth_B.
InterPro	IPR002300. aa-tRNA-synth_Ia. IPR002302. Leu-tRNA-ligase_bac/mito. IPR025709. Leu_tRNA-synth_edit. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. [Graphical view]
PANTHER	PTHR11946:SF7. PTHR11946:SF7. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 3 hits. PF13603. tRNA-synt_1_2. 1 hit. [Graphical view]
PRINTS	PR00985. TRNASYNTHLEU.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00396. leuS_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B3CQ12_ORITI

Accession

Primary (citable) accession number: B3CQ12

Entry history

Integrated into UniProtKB/TrEMBL:	July 22, 2008
Last sequence update:	July 22, 2008
Last modified:	May 1, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)