Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

B3CPK8 (DDL_WOLPP) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:WP0403
OrganismWolbachia pipientis subsp. Culex pipiens (strain wPip) [Complete proteome] [HAMAP]
Taxonomic identifier570417 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeWolbachieaeWolbachia

Protein attributes

Sequence length317 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 317317D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000091215

Regions

Domain103 – 299197ATP-grasp
Nucleotide binding130 – 18354ATP By similarity

Sites

Metal binding2511Magnesium or manganese 1 By similarity
Metal binding2651Magnesium or manganese 1 By similarity
Metal binding2651Magnesium or manganese 2 By similarity
Metal binding2671Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
B3CPK8 [UniParc].

Last modified July 22, 2008. Version 1.
Checksum: C60A735F1A8ABDB8

FASTA31735,815
        10         20         30         40         50         60 
MLMVPNIAIL SGGFSCEREI SLMSGKAVKK ALDSLSYNAI EIDVDSNIAQ KLKKTNPALA 

        70         80         90        100        110        120 
FIALHGPYGE DGCIQGLLEI LGIKYTHSEV MASAVAMNKV MSKHIFHSLN IDTPRGYVIS 

       130        140        150        160        170        180 
REDVLKNNIK VDYPYVLKPI NEGSSIGVHM IFSHEDYLEL KNNSSTIMEK MIIEEYIPGI 

       190        200        210        220        230        240 
ELHTAVLLNE AIGTMEIRPK NKFYDYEAKY TDGFAEHIFP AEIPNNIYRI TLEHALKVHQ 

       250        260        270        280        290        300 
FLGCKTVSRS DFRYNPQNNT LKMLEVNTHP GFTELSLVPE IAKLTRGIDF NELVKIIVED 

       310 
SLHHRNIRDQ ADVEQCY 

« Hide

References

[1]"Genome evolution of Wolbachia strain wPip from the Culex pipiens group."
Klasson L., Walker T., Sebaihia M., Sanders M.J., Quail M.A., Lord A., Sanders S., Earl J., O'Neill S.L., Thomson N., Sinkins S.P., Parkhill J.
Mol. Biol. Evol. 25:1877-1887(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: wPip.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM999887 Genomic DNA. Translation: CAQ54511.1.
RefSeqYP_001975187.1. NC_010981.1.

3D structure databases

ProteinModelPortalB3CPK8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING570417.WPa_0403.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAQ54511; CAQ54511; WP0403.
GeneID6384370.
KEGGwpi:WPa_0403.
PATRIC24026390. VBIWolEnd95846_0446.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
KOK01921.
OMAWREAARY.
OrthoDBEOG6ND0KB.
ProtClustDBPRK01372.

Enzyme and pathway databases

BioCycWEND570417:GHSW-407-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_WOLPP
AccessionPrimary (citable) accession number: B3CPK8
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 22, 2008
Last modified: February 19, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways