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Protein

Lipoyl synthase

Gene

lipA

Organism
Wolbachia pipientis subsp. Culex pipiens (strain wPip)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N6-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = protein N6-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Octanoyltransferase (lipB)
  2. Lipoyl synthase (lipA)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi34Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi39Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi45Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi60Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi64Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi67Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionTransferase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lip-synUniRule annotation
Short name:
LSUniRule annotation
Lipoate synthaseUniRule annotation
Lipoic acid synthaseUniRule annotation
Sulfur insertion protein LipAUniRule annotation
Gene namesi
Name:lipAUniRule annotation
Ordered Locus Names:WP0235
OrganismiWolbachia pipientis subsp. Culex pipiens (strain wPip)
Taxonomic identifieri570417 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeWolbachieaeWolbachia
Proteomesi
  • UP000008814 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000996411 – 290Lipoyl synthaseAdd BLAST290

Interactioni

Protein-protein interaction databases

STRINGi570417.WPa_0235.

Structurei

3D structure databases

ProteinModelPortaliB3CP16.
SMRiB3CP16.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0G. Bacteria.
COG0320. LUCA.
KOiK03644.
OMAiGRCPNRG.
OrthoDBiPOG091H069D.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR031691. LIAS_N.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
PfamiView protein in Pfam
PF16881. LIAS_N. 1 hit.
PF04055. Radical_SAM. 1 hit.
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SFLDiSFLDG01058. lipoyl_synthase_like. 1 hit.
SMARTiView protein in SMART
SM00729. Elp3. 1 hit.
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

B3CP16-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHSKPTWLRA KAPAGKVFNE TLNTVKLHNL HTVCEEAACP NIGECWNKRH
60 70 80 90 100
ATVMILGSVC TRACAFCNVA TGIPDKLDPH EPENLAKAIK KLNLKHVVIT
110 120 130 140 150
SVDRDDLSDG GANQFIRCIE EIRKITSETT IEILTPDFLN KKGAFEAIAI
160 170 180 190 200
ASPDVYNHNI ETVPRLYAKI RPRARYFHSL YLLKMVKQIN PKLFTKSGLM
210 220 230 240 250
VGLGETKEEI FQVMDDLRSA EVDFITIGQY LQPTPKHAKI DRYVTPEEFE
260 270 280 290
HYKYIAYSKG FLVVASSPLT RSSYHAEEDF NRLAAAKTST
Length:290
Mass (Da):32,679
Last modified:July 22, 2008 - v1
Checksum:iFC177408359DE40E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM999887 Genomic DNA. Translation: CAQ54343.1.
RefSeqiWP_007302639.1. NC_010981.1.

Genome annotation databases

EnsemblBacteriaiCAQ54343; CAQ54343; WP0235.
GeneIDi33018918.
KEGGiwpi:WP0235.

Similar proteinsi

Entry informationi

Entry nameiLIPA_WOLPP
AccessioniPrimary (citable) accession number: B3CP16
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 22, 2008
Last modified: September 27, 2017
This is version 63 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families