B3CNL3 (B3CNL3_WOLPP) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061
Short name=CMK HAMAP MF_00061
EC=2.7.1.148 HAMAP MF_00061

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase HAMAP MF_00061

Gene names

Name:	ispE HAMAP MF_00061 EMBL CAQ54282.1
Ordered Locus Names:	WP0174

Organism

Wolbachia pipientis subsp. Culex pipiens (strain wPip) [Complete proteome] [HAMAP]

Taxonomic identifier

570417 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Wolbachieae › Wolbachia

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily. HAMAP MF_00061

Ontologies

Keywords
Biological process	Isoprene biosynthesis HAMAP MF_00061
Ligand	ATP-binding HAMAP MF_00061 RuleBase RU003563 Nucleotide-binding
Molecular function	Kinase HAMAP MF_00061 RuleBase RU003563 EMBL CAQ54282.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	100 – 110	11	ATP By similarity HAMAP MF_00061
Sites
	Active site	11	1	By similarity HAMAP MF_00061
	Active site	140	1	By similarity HAMAP MF_00061

Sequences

	Sequence		Length	Mass (Da)
	B3CNL3 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 3B9138F4EA312706	FASTA	288	32,178
	10 20 30 40 50 60 MESFCVKAPA KINLFLHIIN REERGYHLIE SLVVFAKLSN FLEIKVGEKN FRYDNSTVEF 70 80 90 100 110 120 VNSEFRINYR YNTITRAVSL LLRHAPVRTK VTVKVVKNVP IAAGFGSGSS DAGAVIRMLG 130 140 150 160 170 180 ELWNIDRQFL NDIALSVGAD VPASIDSKPV FVRGVGEELC PIKQCSLPTS LVLVKPKKKF 190 200 210 220 230 240 LNTSEVFSKY KEDFSQPIKW SDNTEENLLE LLKETKNDLQ EAAISLVPEI QNVISTLESQ 250 260 270 280 EGSILSRMSG SGVGCFGMFD SEESAKAAAA NIKKEYPEWW VCDTELMV

References

[1]

"Genome evolution of Wolbachia strain wPip from the Culex pipiens group." Klasson L., Walker T., Sebaihia M., Sanders M.J., Quail M.A., Lord A., Sanders S., Earl J., O'Neill S.L., Thomson N., Sinkins S.P., Parkhill J. Mol. Biol. Evol. 25:1877-1887(2008) [PubMed: 18550617] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM999887 Genomic DNA. Translation: CAQ54282.1.
RefSeq	YP_001974990.1. NC_010981.1.
3D structure databases
ProteinModelPortal	B3CNL3.
ModBase	Search...
Protein-protein interaction databases
STRING	B3CNL3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBWOLT00000000158; EBWOLP00000000158; EBWOLG00000000158.
GeneID	6384519.
GenomeReviews	Gene locus WP0174 in contig AM999887_GR.
KEGG	wpi:WPa_0174.
PATRIC	24025880. VBIWolEnd95846_0193.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000030880.
HOGENOM	HBG734593.
OMA	CQLARMS.
ProtClustDB	PRK14610.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B3CNL3_WOLPP

Accession

Primary (citable) accession number: B3CNL3

Entry history

Integrated into UniProtKB/TrEMBL:	July 22, 2008
Last sequence update:	July 22, 2008
Last modified:	December 14, 2011
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)