B3CML1 (FABH_WOLPP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-oxoacyl-[acyl-carrier-protein] synthase 3
EC=2.3.1.180

Alternative name(s):
3-oxoacyl-[acyl-carrier-protein] synthase III
Beta-ketoacyl-ACP synthase III
Short name=KAS III

Gene names

Name:	fabH
Ordered Locus Names:	WP1025

Organism

Wolbachia pipientis subsp. Culex pipiens (strain wPip) [Complete proteome] [HAMAP]

Taxonomic identifier

570417 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Wolbachieae › Wolbachia

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Its substrate specificity determines the biosynthesis of branched-chain and/or straight-chain of fatty acids By similarity. HAMAP MF_01815
Catalytic activity	Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacetyl-[acyl-carrier-protein] + CoA + CO2. HAMAP MF_01815
Pathway	Lipid metabolism; fatty acid biosynthesis. HAMAP MF_01815
Subunit structure	Homodimer By similarity. HAMAP MF_01815
Subcellular location	Cytoplasm By similarity HAMAP MF_01815.
Domain	The last Arg residue of the ACP-binding site is essential for the weak association between ACP/AcpP and FabH By similarity. HAMAP MF_01815
Sequence similarities	Belongs to the FabH family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-oxoacyl-[acyl-carrier-protein] synthase activity Inferred from electronic annotation. Source: InterPro beta-ketoacyl-acyl-carrier-protein synthase III activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 318	318	3-oxoacyl-[acyl-carrier-protein] synthase 3 HAMAP MF_01815		PRO_1000187910
Regions
	Region	246 – 250	5	ACP-binding By similarity
Sites
	Active site	113	1	By similarity
	Active site	245	1	By similarity
	Active site	275	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3CML1 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 0F857EFA8A9D46C6	FASTA	318	34,427
	10 20 30 40 50 60 MSKSFILSTG SYLPKKILSN NEIALIVETN DEWIRQRTGI VQRHIADEGE LTSDLAVNAA 70 80 90 100 110 120 KNAIEKAKIS IDEIGLIIVA TTTPDKTLPS CATIVQSKLK CKNAFSFDVQ AACSGFIYAV 130 140 150 160 170 180 TVADSLIKSH NRIKYALVIG AEIMSRIVDW EDRSTCVLFG DSAGAVIIKS EMGSSGIIST 190 200 210 220 230 240 NLHSDGNVDI LCTNGGVSST RDSGKILMNG REVFKHAVDK LTASVEETLK CNNLKIIDID 250 260 270 280 290 300 WLIPHQANIR IIEAVVKKLD FPIEKVINTV DKHANTSAAS IPLALDYAIQ ESKIKSGNLV 310 LLISIGAGLT WGSVLLRY

References

[1]

"Genome evolution of Wolbachia strain wPip from the Culex pipiens group." Klasson L., Walker T., Sebaihia M., Sanders M.J., Quail M.A., Lord A., Sanders S., Earl J., O'Neill S.L., Thomson N., Sinkins S.P., Parkhill J. Mol. Biol. Evol. 25:1877-1887(2008) [PubMed: 18550617] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: wPip.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM999887 Genomic DNA. Translation: CAQ55133.1.
RefSeq	YP_001975771.1. NC_010981.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B3CML1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBWOLT00000001129; EBWOLP00000001129; EBWOLG00000001129.
GeneID	6385325.
GenomeReviews	Gene locus WP1025 in contig AM999887_GR.
KEGG	wpi:WPa_1025.
PATRIC	24027779. VBIWolEnd95846_1118.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000031462.
HOGENOM	HBG649927.
OMA	DAYIRGG.
ProtClustDB	CLSK739999.
Family and domain databases
HAMAP	MF_01815. FabH. [Tree]
InterPro	IPR013751. ACP_syn_III. IPR013747. ACP_syn_III_C. IPR004655. FabH_synth. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 2 hits.
KO	K00648.
Pfam	PF08545. ACP_syn_III. 1 hit. PF08541. ACP_syn_III_C. 1 hit. [Graphical view]
SUPFAM	SSF53901. Thiolase-like. 1 hit.
TIGRFAMs	TIGR00747. FabH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FABH_WOLPP

Accession

Primary (citable) accession number: B3CML1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	July 22, 2008
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program