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B3CLT1 (SYL_WOLPP) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:WP0741
OrganismWolbachia pipientis subsp. Culex pipiens (strain wPip) [Complete proteome] [HAMAP]
Taxonomic identifier570417 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeWolbachieaeWolbachia

Protein attributes

Sequence length819 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 819819Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091378

Regions

Motif36 – 4611"HIGH" region HAMAP-Rule MF_00049
Motif586 – 5905"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5891ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B3CLT1 [UniParc].

Last modified July 22, 2008. Version 1.
Checksum: 5D9B6B6366227F7D

FASTA81994,306
        10         20         30         40         50         60 
MKYDFKNVEK FYQNKWDFSV SKDSKKKKCY VLEMFPYPSG KIHMGHLRNY AIGDVIARYK 

        70         80         90        100        110        120 
RARGFEVLHP IGWDAFGLPA ENAARDNNIN PAAWTQDNID NMRTQLKSIG LSYNWDRELS 

       130        140        150        160        170        180 
TCEPDYYKHE QKFFLDFLKH GLAYRKESWV NWDPVDQTVL ANEQVVDGKG WRSGAVVEKR 

       190        200        210        220        230        240 
KLFQWFLKIT DFAEDLLHCL QSLKNWPEKV KTMQERWIGK SEGATIDFEI FGLNKKLKIF 

       250        260        270        280        290        300 
TTSPHTLFGA SFLAVGAEHP IVQDLKDKEI RDFIGNMKAK GENDEKIGIY TGLNVKHPFL 

       310        320        330        340        350        360 
DKELPLYIAN FVLMEYGEGA IFGCPAHDQR DFEFAQKYGL PIIPVVCEES TEILKEPYFG 

       370        380        390        400        410        420 
DGVMFNSEFL NGLMINEAKK VIIKKLEEKG IGKKTINYRL HDWGISRQRY WGCPIPIIHC 

       430        440        450        460        470        480 
KDCGIVPVPE KDLPVVLPTD VEFTSGGNPL DKHPTWKFVD CPKCGKQAER ETDTFDTFFE 

       490        500        510        520        530        540 
SSWYFAAFCS ENKSINKDTC NRFMPVDYYI GGIEHAILHL LYSRFFCRAL TKCGYFDVKE 

       550        560        570        580        590        600 
PFSTLITQGM VCHITYKDEN GKWLFPEEAK ELIAKGAKIQ VGKVEKMSKS KKNTVDPNFI 

       610        620        630        640        650        660 
IEKYGADTAR LFVLSDTPPE KDMEWSDDGV EGCFRYINKL WRMVVQLRTV NIHYDNENIV 

       670        680        690        700        710        720 
GKLLEYRKKI HKLLHGLTDD LENCRLNCVV AKFREMTNLI AEIDVKTGKS LIDEGICILI 

       730        740        750        760        770        780 
RVIEPLMPHL AENLWQEIGG EGMLYMQPWP KADESLLIDN MVTVAVQING KLRATIKVET 

       790        800        810 
DLPQEELKKI ATDSVSNKID QSKIRTIYAV PNKVVNIVI 

« Hide

References

[1]"Genome evolution of Wolbachia strain wPip from the Culex pipiens group."
Klasson L., Walker T., Sebaihia M., Sanders M.J., Quail M.A., Lord A., Sanders S., Earl J., O'Neill S.L., Thomson N., Sinkins S.P., Parkhill J.
Mol. Biol. Evol. 25:1877-1887(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: wPip.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM999887 Genomic DNA. Translation: CAQ54849.1.
RefSeqYP_001975501.1. NC_010981.1.

3D structure databases

ProteinModelPortalB3CLT1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING570417.WPa_0741.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAQ54849; CAQ54849; WP0741.
GeneID6384619.
KEGGwpi:WPa_0741.
PATRIC24027156. VBIWolEnd95846_0819.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
KOK01869.
OMAPVIHCPK.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycWEND570417:GHSW-755-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_WOLPP
AccessionPrimary (citable) accession number: B3CLT1
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 22, 2008
Last modified: May 14, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries