UniProtKB - B3CLF5 (SYE2_WOLPP)
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Protein
Glutamate--tRNA ligase 2
Gene
gltX2
Organism
Wolbachia pipientis subsp. Culex pipiens (strain wPip)
Status
Functioni
Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation
Catalytic activityi
ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 235 | ATPUniRule annotation | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- glutamate-tRNA ligase activity Source: UniProtKB-EC
- tRNA binding Source: InterPro
- zinc ion binding Source: InterPro
GO - Biological processi
- glutamyl-tRNA aminoacylation Source: InterPro
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Glutamate--tRNA ligase 2UniRule annotation (EC:6.1.1.17UniRule annotation)Alternative name(s): Glutamyl-tRNA synthetase 2UniRule annotation Short name: GluRS 2UniRule annotation |
Gene namesi | Name:gltX2UniRule annotation Ordered Locus Names:WP0615 |
Organismi | Wolbachia pipientis subsp. Culex pipiens (strain wPip) |
Taxonomic identifieri | 570417 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Wolbachieae › Wolbachia › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000367795 | 1 – 450 | Glutamate--tRNA ligase 2Add BLAST | 450 |
Interactioni
Subunit structurei
Monomer.UniRule annotation
Protein-protein interaction databases
STRINGi | 570417.WPa_0615. |
Structurei
3D structure databases
ProteinModelPortali | B3CLF5. |
SMRi | B3CLF5. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 10 – 20 | "HIGH" regionUniRule annotationAdd BLAST | 11 | |
Motifi | 232 – 236 | "KMSKS" regionUniRule annotation | 5 |
Sequence similaritiesi
Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation
Phylogenomic databases
eggNOGi | ENOG4105C20. Bacteria. COG0008. LUCA. |
KOi | K01885. |
OMAi | AYYAFDT. |
OrthoDBi | POG091H021W. |
Family and domain databases
CDDi | cd00808. GluRS_core. 1 hit. |
Gene3Di | 1.10.10.350. 1 hit. 3.40.50.620. 1 hit. |
HAMAPi | MF_00022. Glu_tRNA_synth_type1. 1 hit. |
InterProi | View protein in InterPro IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-ligase_bac/mito. IPR000924. Glu/Gln-tRNA-synth. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR033910. GluRS_core. IPR014729. Rossmann-like_a/b/a_fold. |
Pfami | View protein in Pfam PF00749. tRNA-synt_1c. 1 hit. |
PRINTSi | PR00987. TRNASYNTHGLU. |
SUPFAMi | SSF48163. SSF48163. 1 hit. |
TIGRFAMsi | TIGR00464. gltX_bact. 1 hit. |
PROSITEi | View protein in PROSITE PS00178. AA_TRNA_LIGASE_I. 1 hit. |
i Sequence
Sequence statusi: Complete.
B3CLF5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPGIVTRFAP SPTGFLHIGG ARTALFNWLY AQRHGGQFLL RIEDTDRKRS
60 70 80 90 100
TQEAIDAIID GLKWLGISYD GEIVYQSKRI DRHIEVANLL VEKGRAYHCY
110 120 130 140 150
CPENEVAEKK IRAREEGKIY KHKCTHSTSN AEPVVRFKVP DSEDIVVDDK
160 170 180 190 200
IYGQVTISSD QLDDIVILRS DNTPTYIFAV VVDDHDAGIT DIIRGSDHLT
210 220 230 240 250
NTFKQLLIYQ ALDFDVPRFA HVPLIHGEDG NKLSKRHGAT SVCDYEKMGI
260 270 280 290 300
LPQAMRNYLL RLGWSHGNDE IISDEQAIEW FNLESIGRSP ARLDFKKLEH
310 320 330 340 350
LNNHYISNMS NEDILTLMLR ENTLTDKKKG YLLQGLTELK KRANYLTELL
360 370 380 390 400
DLAKFYIQVD LSEEAQQIVK SNLNVIKLLA SFLSSVGSED WNKNFLSSQI
410 420 430 440 450
KEFSKLHNIK MSDIYHSLRA PITGIMDAPG IIDIMVILGK DECIKRLQAI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AM999887 Genomic DNA. Translation: CAQ54723.1. |
RefSeqi | WP_007302040.1. NC_010981.1. |
Genome annotation databases
EnsemblBacteriai | CAQ54723; CAQ54723; WP0615. |
GeneIDi | 33019273. |
KEGGi | wpi:WP0615. |
Similar proteinsi
Entry informationi
Entry namei | SYE2_WOLPP | |
Accessioni | B3CLF5Primary (citable) accession number: B3CLF5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 24, 2009 |
Last sequence update: | July 22, 2008 | |
Last modified: | February 28, 2018 | |
This is version 65 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |