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B3CLD0 (B3CLD0_WOLPP) Unreviewed, UniProtKB/TrEMBL

Last modified May 29, 2013. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Queuine tRNA-ribosyltransferase HAMAP-Rule MF_00168 RuleBase RU003777
EC=2.4.2.29 HAMAP-Rule MF_00168 RuleBase RU003777
Alternative name(s):
Guanine insertion enzyme HAMAP-Rule MF_00168
tRNA-guanine transglycosylase HAMAP-Rule MF_00168
Gene names
Name:tgt HAMAP-Rule MF_00168 EMBL CAQ54697.1
Ordered Locus Names:WP0589
OrganismWolbachia pipientis subsp. Culex pipiens (strain wPip) [Complete proteome] [HAMAP]
Taxonomic identifier570417 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Wolbachieae › Wolbachia ›

Protein attributes

Sequence length403 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity. HAMAP-Rule MF_00168 RuleBase RU003777 SAAS SAAS004803

Catalytic activity

Guanine34 in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine34 in tRNA + guanine. HAMAP-Rule MF_00168 SAAS SAAS004803

Guanine34 in tRNA + queuine = queuosine34 in tRNA + guanine. HAMAP-Rule MF_00168 SAAS SAAS004803

[tRNA]-guanine + queuine = [tRNA]-queuine + guanine. RuleBase RU003777

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00168 RuleBase RU003777 SAAS SAAS004803

Pathway

tRNA modification; tRNA-queuosine biosynthesis. HAMAP-Rule MF_00168 SAAS SAAS004803

Sequence similarities

Belongs to the queuine tRNA-ribosyltransferase family. HAMAP-Rule MF_00168 RuleBase RU003777

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site921Nucleophile By similarity HAMAP-Rule MF_00168
Metal binding3321Zinc By similarity HAMAP-Rule MF_00168
Metal binding3341Zinc By similarity HAMAP-Rule MF_00168
Metal binding3371Zinc By similarity HAMAP-Rule MF_00168
Metal binding3631Zinc By similarity HAMAP-Rule MF_00168
Binding site931Substrate By similarity HAMAP-Rule MF_00168

Sequences

Sequence LengthMass (Da)Tools
B3CLD0 [UniParc].

Last modified July 22, 2008. Version 1.
Checksum: C8D2B56E937DE0DA

FASTA40345,644
        10         20         30         40         50         60 
MEKFSFRITK QSGSARVGTI KTPNGSIETP AFIFCATKAA IKAADIERIR EAGTQIILSN 

        70         80         90        100        110        120 
TYHLMLQPGE DTVAKLGGLH KMMGWSGPML TDSGGYQIFS LGHGSVSEEI KGIRQKQKTL 

       130        140        150        160        170        180 
IKINEDGAIF RSYINGKTYC LTPEKSIQIQ QKLGADLILV LDECTPFHVS KEYTRKSMLM 

       190        200        210        220        230        240 
SHRWAERSLN KFEKNNDGKQ ALYGISQGGV YQDLRRESCD FINDLPFFGQ AIGGSLGQSK 

       250        260        270        280        290        300 
EQMYDVVSFT MEHLKKDRPT HLLGIGGIVD IFRGVELGVD TFDCVHPTRL ARHGGALVKV 

       310        320        330        340        350        360 
KNRDSISSKR KEHINLRNQR FELDDNPIEN DCLCFTCRKH SRAYIHHLLK AKELLAYTLV 

       370        380        390        400 
TIHNVFFMNK LMESIRQAIL NDRLDQEKNN WISEIPLHCE LYT

« Hide

References

[1]"Genome evolution of Wolbachia strain wPip from the Culex pipiens group."
Klasson L., Walker T., Sebaihia M., Sanders M.J., Quail M.A., Lord A., Sanders S., Earl J., O'Neill S.L., Thomson N., Sinkins S.P., Parkhill J.
Mol. Biol. Evol. 25:1877-1887(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: wPip.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AM999887 Genomic DNA. Translation: CAQ54697.1.
RefSeqYP_001975357.1. NC_010981.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRING570417.WPa_0589.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAQ54697; CAQ54697; WP0589.
GeneID6385044.
KEGGwpi:WPa_0589.
PATRIC24026808. VBIWolEnd95846_0651.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0343.
KOK00773.
OMAMSPERSI.
ProtClustDBCLSK749647.

Enzyme and pathway databases

BioCycWEND570417:GHSW-597-MONOMER.
UniPathwayUPA00392.

Family and domain databases

Gene3D3.20.20.105. 1 hit.
HAMAPMF_00168. Q_tRNA_Tgt.
InterProIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PANTHERPTHR11962. PTHR11962. 1 hit.
PfamPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMSSF51713. tRNA_ribo_trans. 1 hit.
TIGRFAMsTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNetSearch...

Entry information

Entry nameB3CLD0_WOLPP
AccessionPrimary (citable) accession number: B3CLD0
Entry history
Integrated into UniProtKB/TrEMBL: July 22, 2008
Last sequence update: July 22, 2008
Last modified: May 29, 2013
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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