B3CLA3 (EFG_WOLPP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor G
Short name=EF-G

Gene names

Name:	fusA
Ordered Locus Names:	WP0562

Organism

Wolbachia pipientis subsp. Culex pipiens (strain wPip) [Complete proteome] [HAMAP]

Taxonomic identifier

570417 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Wolbachieae › Wolbachia

Protein attributes

Sequence length	691 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome By similarity. HAMAP MF_00054_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00054_B.
Sequence similarities	Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Elongation factor
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro translation elongation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 691	691	Elongation factor G HAMAP MF_00054_B		PRO_1000091779
Regions
	Nucleotide binding	15 – 22	8	GTP By similarity
	Nucleotide binding	79 – 83	5	GTP By similarity
	Nucleotide binding	133 – 136	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3CLA3 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 297F86348B8A728A	FASTA	691	76,474
	10 20 30 40 50 60 MEVLISRYRN IGIMAHIDAG KTTTTERILF YTGKQNRIGE VHDGAASMDW MEQEKERGIT 70 80 90 100 110 120 ITSAATTCFW NDHRINIIDT PGHVDFTIEV ERSLRVLDGA VAVFDGVAGV EPQSETVWRQ 130 140 150 160 170 180 ADKYNVPRIC FVNKMDRIGA NFYRCVDMIK TKLGASPLVI QLPIGSEKDF KGIIDLISMK 190 200 210 220 230 240 AIIWQEETLG AKFSYEDIPS DLLDKAQEYR NLLLDAAAEM DDEAINTYFE SNDLPIDLLK 250 260 270 280 290 300 KCVRSGTIKG KFVPVLCGSA FKNKGVQSLL DGVVDFLPSP IDVDVIIGTD PKDSEKKIEI 310 320 330 340 350 360 KPSEKEKFVA LAFKVMTDKF VGSLTFIRIY SGKLKSKSAV LNAGKNETEG IGRMLLMHAN 370 380 390 400 410 420 NREDINEAKV GDIVALVGLK KTITGDTLCS SDFPILLERM EFPDPVIEIA IEPKTTSDQE 430 440 450 460 470 480 KLGVALNRLV AEDPSLRMSV NAESGQTILK GMGELHLEII IDRMKREFNV EANVGAPQVA 490 500 510 520 530 540 YRETITKSVE IDYTHKKQSG GAGQFAKVKI KFEPLEPGFG FQFESKIVGG AIPKEYIPGV 550 560 570 580 590 600 QNGLELIKEG GIISGFPLID FKATLLDGAF HDVDSSPLAF ELAAKGAFKE MANKAGPKML 610 620 630 640 650 660 EPIMKVEIIT PEEYMGDVMG DINSRRGSVV DMLDLGNNSK IITASVPLAN MFGYINVLRS 670 680 690 ISQGRAQYSM HFSCYEQVPQ YVVDELKLKY N

References

[1]

"Genome evolution of Wolbachia strain wPip from the Culex pipiens group." Klasson L., Walker T., Sebaihia M., Sanders M.J., Quail M.A., Lord A., Sanders S., Earl J., O'Neill S.L., Thomson N., Sinkins S.P., Parkhill J. Mol. Biol. Evol. 25:1877-1887(2008) [PubMed: 18550617] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: wPip.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM999887 Genomic DNA. Translation: CAQ54670.1.
RefSeq	YP_001975334.1. NC_010981.1.
3D structure databases
ProteinModelPortal	B3CLA3.
ModBase	Search...
Protein-protein interaction databases
STRING	B3CLA3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBWOLT00000001153; EBWOLP00000001153; EBWOLG00000001153.
GeneID	6385309.
GenomeReviews	Gene locus WP0562 in contig AM999887_GR.
KEGG	wpi:WPa_0562.
PATRIC	24026746. VBIWolEnd95846_0621.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000030977.
HOGENOM	HBG737692.
OMA	CNEWREK.
ProtClustDB	PRK00007.
Family and domain databases
HAMAP	MF_00054_B. EF-G_EF-2_B. [Tree]
InterPro	IPR009022. Elongation_fac_G/III/V. IPR000795. ProtSyn_GTP-bd. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR005225. Small_GTP-bd_dom. IPR004540. Transl_elong_EFG/EF2. IPR000640. Transl_elong_EFG/EF2_C. IPR005517. Transl_elong_EFG/EF2_IV. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit. G3DSA:3.30.70.240. Transl_elong_EFG/EF2_C. 1 hit.
KO	K02355.
Pfam	PF00679. EFG_C. 1 hit. PF03764. EFG_IV. 1 hit. PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SMART	SM00838. EFG_C. 1 hit. SM00889. EFG_IV. 1 hit. [Graphical view]
SUPFAM	SSF54980. EFG_III_V. 2 hits. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00484. EF-G. 1 hit. TIGR00231. Small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EFG_WOLPP

Accession

Primary (citable) accession number: B3CLA3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 22, 2008
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program